Combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidney
AbstractInflammatory response and oxidative stress state have been largely described in diabetes and depression separately but not in combination. We aimed to explore the involvement of diabetes and unpredictable chronic mild stress (UCMS) separately or in combination on inflammatory response and ox...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2023.2182137 |
| _version_ | 1797367474094604288 |
|---|---|
| author | Houcine Dab Said Ben Hamed Wassim Hodroj Lazhar Zourgui |
| author_facet | Houcine Dab Said Ben Hamed Wassim Hodroj Lazhar Zourgui |
| author_sort | Houcine Dab |
| collection | DOAJ |
| description | AbstractInflammatory response and oxidative stress state have been largely described in diabetes and depression separately but not in combination. We aimed to explore the involvement of diabetes and unpredictable chronic mild stress (UCMS) separately or in combination on inflammatory response and oxidative stress. Diabetes, UCMS or combined rat models were used. Proinflammatory cytokines, Interleukin 6 (IL-6) and Tumor necrosis factor alpha (TNF-α) were assessed by real time quantitative polymerase chain reaction (q-PCR) and Enzyme-Linked Immunosorbent Assay (ELISA). Superoxide dismutase (SOD) and catalase (CAT) were determined by colorimetric assays. In the diabetes group, IL-6 mRNA expression increased by 55% and 34% (p < 0.05), respectively, in liver and kidney. UCMS alone or in combination with diabetes increased the mRNA of IL-6, respectively, by 85% and 78% in the liver and by 28% and 63% in the kidney (p < 0.01 for all). ELISA showed that diabetes and UCMS act in synergy on TNF-α and IL-6 expression. Diabetes and UCMS separately or in combination inhibited significantly (p < 0.01) the activities of the two anti-oxidant enzymes when compared to controls. The malondialdehyde (MDA) level was significantly enhanced in the group with diabetes combined with UCMS compared to UCMS alone in both organs. UCMS enhances the proinflammatory cytokines release and induces oxidative stress imbalance, and diabetes comorbidity with depression aggravates the inflammatory response and lipid peroxidation. These observations can be useful to better understand depression-induced organ damage. |
| first_indexed | 2024-03-08T17:18:48Z |
| format | Article |
| id | doaj.art-5cf10feb12294b0886a2630d8f3aff41 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-03-08T17:18:48Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-5cf10feb12294b0886a2630d8f3aff412024-01-03T09:44:12ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302023-12-0137125025910.1080/13102818.2023.2182137Combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidneyHoucine Dab0Said Ben Hamed1Wassim Hodroj2Lazhar Zourgui3Research Unit of Valorization of Active Biomolecules, Higher Institute of Applied Biology Medenine, University of Gabes, Medenine, TunisiaLaboratory of Epidemiology and Veterinary Microbiology (LEMV), Institut Pasteur de Tunis, University of Tunis El Manar, Tunis, TunisiaAcadémie de Versailles, University of Versailles, Athis-Mons, FranceResearch Unit of Valorization of Active Biomolecules, Higher Institute of Applied Biology Medenine, University of Gabes, Medenine, TunisiaAbstractInflammatory response and oxidative stress state have been largely described in diabetes and depression separately but not in combination. We aimed to explore the involvement of diabetes and unpredictable chronic mild stress (UCMS) separately or in combination on inflammatory response and oxidative stress. Diabetes, UCMS or combined rat models were used. Proinflammatory cytokines, Interleukin 6 (IL-6) and Tumor necrosis factor alpha (TNF-α) were assessed by real time quantitative polymerase chain reaction (q-PCR) and Enzyme-Linked Immunosorbent Assay (ELISA). Superoxide dismutase (SOD) and catalase (CAT) were determined by colorimetric assays. In the diabetes group, IL-6 mRNA expression increased by 55% and 34% (p < 0.05), respectively, in liver and kidney. UCMS alone or in combination with diabetes increased the mRNA of IL-6, respectively, by 85% and 78% in the liver and by 28% and 63% in the kidney (p < 0.01 for all). ELISA showed that diabetes and UCMS act in synergy on TNF-α and IL-6 expression. Diabetes and UCMS separately or in combination inhibited significantly (p < 0.01) the activities of the two anti-oxidant enzymes when compared to controls. The malondialdehyde (MDA) level was significantly enhanced in the group with diabetes combined with UCMS compared to UCMS alone in both organs. UCMS enhances the proinflammatory cytokines release and induces oxidative stress imbalance, and diabetes comorbidity with depression aggravates the inflammatory response and lipid peroxidation. These observations can be useful to better understand depression-induced organ damage.https://www.tandfonline.com/doi/10.1080/13102818.2023.2182137Diabetesunpredictable chronic mild stressproinflammatory cytokineoxidative stress |
| spellingShingle | Houcine Dab Said Ben Hamed Wassim Hodroj Lazhar Zourgui Combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidney Biotechnology & Biotechnological Equipment Diabetes unpredictable chronic mild stress proinflammatory cytokine oxidative stress |
| title | Combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidney |
| title_full | Combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidney |
| title_fullStr | Combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidney |
| title_full_unstemmed | Combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidney |
| title_short | Combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidney |
| title_sort | combined diabetes and chronic stress exacerbates cytokine production and oxidative stress in rat liver and kidney |
| topic | Diabetes unpredictable chronic mild stress proinflammatory cytokine oxidative stress |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2023.2182137 |
| work_keys_str_mv | AT houcinedab combineddiabetesandchronicstressexacerbatescytokineproductionandoxidativestressinratliverandkidney AT saidbenhamed combineddiabetesandchronicstressexacerbatescytokineproductionandoxidativestressinratliverandkidney AT wassimhodroj combineddiabetesandchronicstressexacerbatescytokineproductionandoxidativestressinratliverandkidney AT lazharzourgui combineddiabetesandchronicstressexacerbatescytokineproductionandoxidativestressinratliverandkidney |