Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation
Protein adsorption is known to direct biological response to biomaterials and is important in determining cellular response in tissue scaffolds. In this study we investigated the effect of the duration of protein adsorption to 3D polymer scaffolds on cell attachment and proliferation. 3D macro-porou...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-05-01
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| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-4983/3/2/372 |
| _version_ | 1798002826273619968 |
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| author | Carl G. Simon Girish Kumar Stevephen Hung Kaushik Chatterjee |
| author_facet | Carl G. Simon Girish Kumar Stevephen Hung Kaushik Chatterjee |
| author_sort | Carl G. Simon |
| collection | DOAJ |
| description | Protein adsorption is known to direct biological response to biomaterials and is important in determining cellular response in tissue scaffolds. In this study we investigated the effect of the duration of protein adsorption to 3D polymer scaffolds on cell attachment and proliferation. 3D macro-porous polymer scaffolds were pre-aged in serum-containing culture medium for 5 min, 1 d or 7 d prior to seeding osteoblasts. The total amount of protein adsorbed was found to increase with pre-ageing time. Cell attachment and proliferation were measured 1 d and 14 d, respectively, after cell seeding. Osteoblast proliferation, but not attachment, increased with scaffold pre-ageing time and amount of adsorbed serum protein. These results demonstrate that the amount of time that scaffolds are exposed to serum-containing medium can affect cell proliferation and suggest that these effects are mediated by differences in the amount of protein adsorption. |
| first_indexed | 2024-04-11T11:59:36Z |
| format | Article |
| id | doaj.art-5cf1577fced6440e9da999eddf998b02 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-11T11:59:36Z |
| publishDate | 2012-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-5cf1577fced6440e9da999eddf998b022022-12-22T04:24:57ZengMDPI AGJournal of Functional Biomaterials2079-49832012-05-013237238110.3390/jfb3020372Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell ProliferationCarl G. SimonGirish KumarStevephen HungKaushik ChatterjeeProtein adsorption is known to direct biological response to biomaterials and is important in determining cellular response in tissue scaffolds. In this study we investigated the effect of the duration of protein adsorption to 3D polymer scaffolds on cell attachment and proliferation. 3D macro-porous polymer scaffolds were pre-aged in serum-containing culture medium for 5 min, 1 d or 7 d prior to seeding osteoblasts. The total amount of protein adsorbed was found to increase with pre-ageing time. Cell attachment and proliferation were measured 1 d and 14 d, respectively, after cell seeding. Osteoblast proliferation, but not attachment, increased with scaffold pre-ageing time and amount of adsorbed serum protein. These results demonstrate that the amount of time that scaffolds are exposed to serum-containing medium can affect cell proliferation and suggest that these effects are mediated by differences in the amount of protein adsorption.http://www.mdpi.com/2079-4983/3/2/3723D scaffoldsbiomaterialsosteoblastprotein adsorptiontissue engineering |
| spellingShingle | Carl G. Simon Girish Kumar Stevephen Hung Kaushik Chatterjee Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation Journal of Functional Biomaterials 3D scaffolds biomaterials osteoblast protein adsorption tissue engineering |
| title | Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation |
| title_full | Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation |
| title_fullStr | Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation |
| title_full_unstemmed | Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation |
| title_short | Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation |
| title_sort | time dependent effects of pre aging 3d polymer scaffolds in cell culture medium on cell proliferation |
| topic | 3D scaffolds biomaterials osteoblast protein adsorption tissue engineering |
| url | http://www.mdpi.com/2079-4983/3/2/372 |
| work_keys_str_mv | AT carlgsimon timedependenteffectsofpreaging3dpolymerscaffoldsincellculturemediumoncellproliferation AT girishkumar timedependenteffectsofpreaging3dpolymerscaffoldsincellculturemediumoncellproliferation AT stevephenhung timedependenteffectsofpreaging3dpolymerscaffoldsincellculturemediumoncellproliferation AT kaushikchatterjee timedependenteffectsofpreaging3dpolymerscaffoldsincellculturemediumoncellproliferation |