Identification and Characterization of UDP-Glycosyltransferase Genes in a Cerambycid Beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae)
The cerambycid beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae), is a wood-boring pest that spends most of its life cycle in the trunks or under the bark of trees. These distinctive biological characteristics make it likely that this beetle will encounter a numbe...
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MDPI AG
2022-04-01
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| author | Ningna Yin Zhengquan Wang Haiyan Xiao Tingting Lu Naiyong Liu |
| author_facet | Ningna Yin Zhengquan Wang Haiyan Xiao Tingting Lu Naiyong Liu |
| author_sort | Ningna Yin |
| collection | DOAJ |
| description | The cerambycid beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae), is a wood-boring pest that spends most of its life cycle in the trunks or under the bark of trees. These distinctive biological characteristics make it likely that this beetle will encounter a number of plant defensive compounds, coupled with a broad range of host plants, possibly resulting in the overexpression or expansion of uridine diphosphate (UDP)-glycosyltransferase (UGT) genes. Here, we identified and characterized the UGT gene family in <i>P. antennata</i> through transcriptome data, sequence and phylogenetic analyses, and PCR and homology modeling approaches. In total, 59 transcripts encoding UGTs were identified, 34 of which harbored full-length sequences and shared high conservation with the UGTs of <i>Anoplophora glabripennis</i>. Of the 34 PantUGTs, only 31.78% amino acid identity was observed on average, but catalytic and sugar binding residues were highly conserved. Phylogenetic analyses revealed four Cerambycidae-specific clades, including 30 members from <i>P. antennata</i>. Combining the transcriptome and PCR data showed that PantUGTs had a wide tissue expression, and the majority of the genes were presented mainly in antennae or abdomens, suggesting their putative roles in olfaction and detoxification. This study provides, for the first time, information on the molecular and genetic basis of <i>P. antennata</i>, greatly enhancing our knowledge of the detoxification-related UGT gene family. |
| first_indexed | 2024-03-10T03:01:51Z |
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| language | English |
| last_indexed | 2024-03-10T03:01:51Z |
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| spelling | doaj.art-5cf2d582f7394446ab47c53b71e447902023-11-23T10:42:52ZengMDPI AGDiversity1424-28182022-04-0114534810.3390/d14050348Identification and Characterization of UDP-Glycosyltransferase Genes in a Cerambycid Beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae)Ningna Yin0Zhengquan Wang1Haiyan Xiao2Tingting Lu3Naiyong Liu4Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaThe cerambycid beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae), is a wood-boring pest that spends most of its life cycle in the trunks or under the bark of trees. These distinctive biological characteristics make it likely that this beetle will encounter a number of plant defensive compounds, coupled with a broad range of host plants, possibly resulting in the overexpression or expansion of uridine diphosphate (UDP)-glycosyltransferase (UGT) genes. Here, we identified and characterized the UGT gene family in <i>P. antennata</i> through transcriptome data, sequence and phylogenetic analyses, and PCR and homology modeling approaches. In total, 59 transcripts encoding UGTs were identified, 34 of which harbored full-length sequences and shared high conservation with the UGTs of <i>Anoplophora glabripennis</i>. Of the 34 PantUGTs, only 31.78% amino acid identity was observed on average, but catalytic and sugar binding residues were highly conserved. Phylogenetic analyses revealed four Cerambycidae-specific clades, including 30 members from <i>P. antennata</i>. Combining the transcriptome and PCR data showed that PantUGTs had a wide tissue expression, and the majority of the genes were presented mainly in antennae or abdomens, suggesting their putative roles in olfaction and detoxification. This study provides, for the first time, information on the molecular and genetic basis of <i>P. antennata</i>, greatly enhancing our knowledge of the detoxification-related UGT gene family.https://www.mdpi.com/1424-2818/14/5/348<i>Pharsalia antennata</i>UDP-glycosyltransferasephylogenetic analysisexpression profileolfaction |
| spellingShingle | Ningna Yin Zhengquan Wang Haiyan Xiao Tingting Lu Naiyong Liu Identification and Characterization of UDP-Glycosyltransferase Genes in a Cerambycid Beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae) Diversity <i>Pharsalia antennata</i> UDP-glycosyltransferase phylogenetic analysis expression profile olfaction |
| title | Identification and Characterization of UDP-Glycosyltransferase Genes in a Cerambycid Beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae) |
| title_full | Identification and Characterization of UDP-Glycosyltransferase Genes in a Cerambycid Beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae) |
| title_fullStr | Identification and Characterization of UDP-Glycosyltransferase Genes in a Cerambycid Beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae) |
| title_full_unstemmed | Identification and Characterization of UDP-Glycosyltransferase Genes in a Cerambycid Beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae) |
| title_short | Identification and Characterization of UDP-Glycosyltransferase Genes in a Cerambycid Beetle, <i>Pharsalia antennata</i> Gahan, 1894 (Coleoptera: Cerambycidae) |
| title_sort | identification and characterization of udp glycosyltransferase genes in a cerambycid beetle i pharsalia antennata i gahan 1894 coleoptera cerambycidae |
| topic | <i>Pharsalia antennata</i> UDP-glycosyltransferase phylogenetic analysis expression profile olfaction |
| url | https://www.mdpi.com/1424-2818/14/5/348 |
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