Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids
Biomedical applications of low-temperature plasmas are of growing interest, especially in the field of plasma-induced anti-tumor effects. The present work is aimed at investigating the regionalized antiproliferative effects of low-temperature plasmas on a multicellular tumor spheroid (MCTS), a model...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2014-01-01
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| Series: | New Journal of Physics |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1367-2630/16/4/043027 |
| _version_ | 1797751512159485952 |
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| author | Joseph-Marie Plewa Mohammed Yousfi Céline Frongia Olivier Eichwald Bernard Ducommun Nofel Merbahi Valérie Lobjois |
| author_facet | Joseph-Marie Plewa Mohammed Yousfi Céline Frongia Olivier Eichwald Bernard Ducommun Nofel Merbahi Valérie Lobjois |
| author_sort | Joseph-Marie Plewa |
| collection | DOAJ |
| description | Biomedical applications of low-temperature plasmas are of growing interest, especially in the field of plasma-induced anti-tumor effects. The present work is aimed at investigating the regionalized antiproliferative effects of low-temperature plasmas on a multicellular tumor spheroid (MCTS), a model that mimics the 3D organization and regionalization of a microtumor region. We report that a low-temperature plasma jet, using helium flow in open air, inhibits HCT116 colon carcinoma MCTS growth in a dose-dependent manner. This growth inhibition is associated with the loss of Ki67, and the regionalized accumulation of DNA damage detected by histone H2AX phosphorylation. This regionalized genotoxic effect leads to massive cell death and loss of the MCTS proliferative region. The use of reactive oxygen species (ROS), scavenger N-acetyl cysteine (NAC) and plasma-conditioned media demonstrate that the ROS generated in the media after exposure to low-temperature plasma play a major role in these observed effects. These findings strengthen the interest in the use of MCTS for the evaluation of antiproliferative strategies, and open new perspectives for studies dedicated to demonstrate the potential of low-temperature plasma in cancer therapy. |
| first_indexed | 2024-03-12T16:49:34Z |
| format | Article |
| id | doaj.art-5d01fe81cb804e998b9129135f31071d |
| institution | Directory Open Access Journal |
| issn | 1367-2630 |
| language | English |
| last_indexed | 2024-03-12T16:49:34Z |
| publishDate | 2014-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | New Journal of Physics |
| spelling | doaj.art-5d01fe81cb804e998b9129135f31071d2023-08-08T11:24:39ZengIOP PublishingNew Journal of Physics1367-26302014-01-0116404302710.1088/1367-2630/16/4/043027Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroidsJoseph-Marie Plewa0Mohammed Yousfi1Céline Frongia2Olivier Eichwald3Bernard Ducommun4Nofel Merbahi5Valérie Lobjois6Université de Toulouse ; LAPLACE-UMR5213, F-31062 Toulouse, France; CNRS; LAPLACE-UMR5213, F-31062 Toulouse, France; Université de Toulouse ; ITAV-USR3505, F-31106 Toulouse, FranceUniversité de Toulouse ; LAPLACE-UMR5213, F-31062 Toulouse, France; CNRS; LAPLACE-UMR5213, F-31062 Toulouse, FranceUniversité de Toulouse ; ITAV-USR3505, F-31106 Toulouse, France; CNRS; ITAV-USR3505, F-31106 Toulouse, FranceUniversité de Toulouse ; LAPLACE-UMR5213, F-31062 Toulouse, France; CNRS; LAPLACE-UMR5213, F-31062 Toulouse, FranceUniversité de Toulouse ; ITAV-USR3505, F-31106 Toulouse, France; CNRS; ITAV-USR3505, F-31106 Toulouse, France; CHU de Toulouse; F-31059 Toulouse, FranceUniversité de Toulouse ; LAPLACE-UMR5213, F-31062 Toulouse, France; CNRS; LAPLACE-UMR5213, F-31062 Toulouse, FranceUniversité de Toulouse ; ITAV-USR3505, F-31106 Toulouse, France; CNRS; ITAV-USR3505, F-31106 Toulouse, FranceBiomedical applications of low-temperature plasmas are of growing interest, especially in the field of plasma-induced anti-tumor effects. The present work is aimed at investigating the regionalized antiproliferative effects of low-temperature plasmas on a multicellular tumor spheroid (MCTS), a model that mimics the 3D organization and regionalization of a microtumor region. We report that a low-temperature plasma jet, using helium flow in open air, inhibits HCT116 colon carcinoma MCTS growth in a dose-dependent manner. This growth inhibition is associated with the loss of Ki67, and the regionalized accumulation of DNA damage detected by histone H2AX phosphorylation. This regionalized genotoxic effect leads to massive cell death and loss of the MCTS proliferative region. The use of reactive oxygen species (ROS), scavenger N-acetyl cysteine (NAC) and plasma-conditioned media demonstrate that the ROS generated in the media after exposure to low-temperature plasma play a major role in these observed effects. These findings strengthen the interest in the use of MCTS for the evaluation of antiproliferative strategies, and open new perspectives for studies dedicated to demonstrate the potential of low-temperature plasma in cancer therapy.https://doi.org/10.1088/1367-2630/16/4/043027low temperature plasmamulti-cellular tumor spheroidantiproliferative effectgenotoxic and cytotoxic effectsreactive oxygen species |
| spellingShingle | Joseph-Marie Plewa Mohammed Yousfi Céline Frongia Olivier Eichwald Bernard Ducommun Nofel Merbahi Valérie Lobjois Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids New Journal of Physics low temperature plasma multi-cellular tumor spheroid antiproliferative effect genotoxic and cytotoxic effects reactive oxygen species |
| title | Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids |
| title_full | Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids |
| title_fullStr | Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids |
| title_full_unstemmed | Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids |
| title_short | Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids |
| title_sort | low temperature plasma induced antiproliferative effects on multi cellular tumor spheroids |
| topic | low temperature plasma multi-cellular tumor spheroid antiproliferative effect genotoxic and cytotoxic effects reactive oxygen species |
| url | https://doi.org/10.1088/1367-2630/16/4/043027 |
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