Molecular profiling of an oleaginous trebouxiophycean alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production
Abstract Background Decreasing fossil fuels and its impact on global warming have led to an increasing demand for its replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for renewable biofuels capable of converting atmospheric CO2 to substantial biomass and valu...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-07-01
|
| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1521-9 |
| _version_ | 1818229121652621312 |
|---|---|
| author | Kashif Mohd Shaikh Asha Arumugam Nesamma Malik Zainul Abdin Pannaga Pavan Jutur |
| author_facet | Kashif Mohd Shaikh Asha Arumugam Nesamma Malik Zainul Abdin Pannaga Pavan Jutur |
| author_sort | Kashif Mohd Shaikh |
| collection | DOAJ |
| description | Abstract Background Decreasing fossil fuels and its impact on global warming have led to an increasing demand for its replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for renewable biofuels capable of converting atmospheric CO2 to substantial biomass and valuable biofuels, which is of great importance for the food and energy industries. Parachlorella kessleri, a marine unicellular green alga belonging to class Trebouxiophyceae, accumulates large amount of lipids under nutrient-deprived conditions. The present study aims to understand the metabolic imprints in order to elucidate the physiological mechanisms of lipid accumulations in this microalga under nutrient deprivation. Results Molecular profiles were obtained using gas chromatography–mass spectrometry (GC–MS) of P. kessleri subjected to nutrient deprivation. Relative quantities of more than 60 metabolites were systematically compared in all the three starvation conditions. Our results demonstrate that in lipid metabolism, the quantities of neutral lipids increased significantly followed by the decrease in other metabolites involved in photosynthesis, and nitrogen assimilation. Nitrogen starvation seems to trigger the triacylglycerol (TAG) accumulation rapidly, while the microalga seems to tolerate phosphorous limitation, hence increasing both biomass and lipid content. The metabolomic and lipidomic profiles have identified a few common metabolites such as citric acid and 2-ketoglutaric acid which play significant role in diverting flux towards acetyl-CoA leading to accumulation of neutral lipids, whereas other molecules such as trehalose involve in cell growth regulation, when subjected to nutrient deprivation. Conclusions Understanding the entire system through qualitative (untargeted) metabolome approach in P. kessleri has led to identification of relevant metabolites involved in the biosynthesis and degradation of precursor molecules that may have potential for biofuel production, aiming towards the vision of tomorrow’s bioenergy needs. |
| first_indexed | 2024-12-12T10:13:34Z |
| format | Article |
| id | doaj.art-5d020e0c1d4e44528c8d264f6b2e5759 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T10:13:34Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-5d020e0c1d4e44528c8d264f6b2e57592022-12-22T00:27:43ZengBMCBiotechnology for Biofuels1754-68342019-07-0112111510.1186/s13068-019-1521-9Molecular profiling of an oleaginous trebouxiophycean alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel productionKashif Mohd Shaikh0Asha Arumugam Nesamma1Malik Zainul Abdin2Pannaga Pavan Jutur3Omics of Algae Group, Integrative Biology, International Centre for Genetic Engineering and BiotechnologyOmics of Algae Group, Integrative Biology, International Centre for Genetic Engineering and BiotechnologyDepartment of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard UniversityOmics of Algae Group, Integrative Biology, International Centre for Genetic Engineering and BiotechnologyAbstract Background Decreasing fossil fuels and its impact on global warming have led to an increasing demand for its replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for renewable biofuels capable of converting atmospheric CO2 to substantial biomass and valuable biofuels, which is of great importance for the food and energy industries. Parachlorella kessleri, a marine unicellular green alga belonging to class Trebouxiophyceae, accumulates large amount of lipids under nutrient-deprived conditions. The present study aims to understand the metabolic imprints in order to elucidate the physiological mechanisms of lipid accumulations in this microalga under nutrient deprivation. Results Molecular profiles were obtained using gas chromatography–mass spectrometry (GC–MS) of P. kessleri subjected to nutrient deprivation. Relative quantities of more than 60 metabolites were systematically compared in all the three starvation conditions. Our results demonstrate that in lipid metabolism, the quantities of neutral lipids increased significantly followed by the decrease in other metabolites involved in photosynthesis, and nitrogen assimilation. Nitrogen starvation seems to trigger the triacylglycerol (TAG) accumulation rapidly, while the microalga seems to tolerate phosphorous limitation, hence increasing both biomass and lipid content. The metabolomic and lipidomic profiles have identified a few common metabolites such as citric acid and 2-ketoglutaric acid which play significant role in diverting flux towards acetyl-CoA leading to accumulation of neutral lipids, whereas other molecules such as trehalose involve in cell growth regulation, when subjected to nutrient deprivation. Conclusions Understanding the entire system through qualitative (untargeted) metabolome approach in P. kessleri has led to identification of relevant metabolites involved in the biosynthesis and degradation of precursor molecules that may have potential for biofuel production, aiming towards the vision of tomorrow’s bioenergy needs.http://link.springer.com/article/10.1186/s13068-019-1521-9MicroalgaeBiofuelsMetabolomicsParachlorella kessleriNutrient deprivation |
| spellingShingle | Kashif Mohd Shaikh Asha Arumugam Nesamma Malik Zainul Abdin Pannaga Pavan Jutur Molecular profiling of an oleaginous trebouxiophycean alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production Biotechnology for Biofuels Microalgae Biofuels Metabolomics Parachlorella kessleri Nutrient deprivation |
| title | Molecular profiling of an oleaginous trebouxiophycean alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production |
| title_full | Molecular profiling of an oleaginous trebouxiophycean alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production |
| title_fullStr | Molecular profiling of an oleaginous trebouxiophycean alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production |
| title_full_unstemmed | Molecular profiling of an oleaginous trebouxiophycean alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production |
| title_short | Molecular profiling of an oleaginous trebouxiophycean alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production |
| title_sort | molecular profiling of an oleaginous trebouxiophycean alga parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production |
| topic | Microalgae Biofuels Metabolomics Parachlorella kessleri Nutrient deprivation |
| url | http://link.springer.com/article/10.1186/s13068-019-1521-9 |
| work_keys_str_mv | AT kashifmohdshaikh molecularprofilingofanoleaginoustrebouxiophyceanalgaparachlorellakesslerisubjectedtonutrientdeprivationforenhancedbiofuelproduction AT ashaarumugamnesamma molecularprofilingofanoleaginoustrebouxiophyceanalgaparachlorellakesslerisubjectedtonutrientdeprivationforenhancedbiofuelproduction AT malikzainulabdin molecularprofilingofanoleaginoustrebouxiophyceanalgaparachlorellakesslerisubjectedtonutrientdeprivationforenhancedbiofuelproduction AT pannagapavanjutur molecularprofilingofanoleaginoustrebouxiophyceanalgaparachlorellakesslerisubjectedtonutrientdeprivationforenhancedbiofuelproduction |