Lipidomics Analysis Explores the Mechanism of Renal Injury in Rat Induced by 3-MCPD
3-monochloropropane-1,2-diol (3-MCPD) is a food-process toxic substance, and its main target organ is the kidney. The present study examined and characterized the nephrotoxicity and the lipidomic mechanisms in a model of kidney injury in Sprague Dawley (SD) rats treated with high (45 mg/kg) and low...
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MDPI AG
2023-05-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/6/479 |
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| author | Tao Wei Na Cao Tiantian Han Yi Chen Xingtao Zhou Liyang Niu Wenting Liu Chang Li |
| author_facet | Tao Wei Na Cao Tiantian Han Yi Chen Xingtao Zhou Liyang Niu Wenting Liu Chang Li |
| author_sort | Tao Wei |
| collection | DOAJ |
| description | 3-monochloropropane-1,2-diol (3-MCPD) is a food-process toxic substance, and its main target organ is the kidney. The present study examined and characterized the nephrotoxicity and the lipidomic mechanisms in a model of kidney injury in Sprague Dawley (SD) rats treated with high (45 mg/kg) and low (30 mg/kg) doses of 3-MCPD. The results showed that the ingestion of 3-MCPD led to a dose-dependent increase in serum creatinine and urea nitrogen levels and histological renal impairment. The oxidative stress indicators (MDA, GSH, T-AOC) in the rat kidney altered in a dose-dependent manner in 3-MCPD groups. The lipidomics analysis revealed that 3-MCPD caused kidney injury by interfering with glycerophospholipid metabolism and sphingolipid metabolism. In addition, 38 lipids were screened as potential biomarkers. This study not only revealed the mechanism of 3-MCPD renal toxicity from the perspective of lipidomics but also provided a new approach to the study of 3-MCPD nephrotoxicity. |
| first_indexed | 2024-03-11T01:52:02Z |
| format | Article |
| id | doaj.art-5d02342897e84a6d9ffdbb6bd5bc0366 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-11T01:52:02Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Toxics |
| spelling | doaj.art-5d02342897e84a6d9ffdbb6bd5bc03662023-11-18T12:54:10ZengMDPI AGToxics2305-63042023-05-0111647910.3390/toxics11060479Lipidomics Analysis Explores the Mechanism of Renal Injury in Rat Induced by 3-MCPDTao Wei0Na Cao1Tiantian Han2Yi Chen3Xingtao Zhou4Liyang Niu5Wenting Liu6Chang Li7State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China3-monochloropropane-1,2-diol (3-MCPD) is a food-process toxic substance, and its main target organ is the kidney. The present study examined and characterized the nephrotoxicity and the lipidomic mechanisms in a model of kidney injury in Sprague Dawley (SD) rats treated with high (45 mg/kg) and low (30 mg/kg) doses of 3-MCPD. The results showed that the ingestion of 3-MCPD led to a dose-dependent increase in serum creatinine and urea nitrogen levels and histological renal impairment. The oxidative stress indicators (MDA, GSH, T-AOC) in the rat kidney altered in a dose-dependent manner in 3-MCPD groups. The lipidomics analysis revealed that 3-MCPD caused kidney injury by interfering with glycerophospholipid metabolism and sphingolipid metabolism. In addition, 38 lipids were screened as potential biomarkers. This study not only revealed the mechanism of 3-MCPD renal toxicity from the perspective of lipidomics but also provided a new approach to the study of 3-MCPD nephrotoxicity.https://www.mdpi.com/2305-6304/11/6/4793-MCPDnephrotoxicitylipidomicsbiomarkerspathway |
| spellingShingle | Tao Wei Na Cao Tiantian Han Yi Chen Xingtao Zhou Liyang Niu Wenting Liu Chang Li Lipidomics Analysis Explores the Mechanism of Renal Injury in Rat Induced by 3-MCPD Toxics 3-MCPD nephrotoxicity lipidomics biomarkers pathway |
| title | Lipidomics Analysis Explores the Mechanism of Renal Injury in Rat Induced by 3-MCPD |
| title_full | Lipidomics Analysis Explores the Mechanism of Renal Injury in Rat Induced by 3-MCPD |
| title_fullStr | Lipidomics Analysis Explores the Mechanism of Renal Injury in Rat Induced by 3-MCPD |
| title_full_unstemmed | Lipidomics Analysis Explores the Mechanism of Renal Injury in Rat Induced by 3-MCPD |
| title_short | Lipidomics Analysis Explores the Mechanism of Renal Injury in Rat Induced by 3-MCPD |
| title_sort | lipidomics analysis explores the mechanism of renal injury in rat induced by 3 mcpd |
| topic | 3-MCPD nephrotoxicity lipidomics biomarkers pathway |
| url | https://www.mdpi.com/2305-6304/11/6/479 |
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