Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing
Ageing is accompanied by dramatic changes in chromatin structure organization and genome function. Two essential components of chromatin, the linker histone Hho1p and actin-related protein 4 (Arp4p), have been shown to physically interact in <i>Saccharomyces cerevisiae</i> cells, thus ma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/17/2754 |
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| author | Mateusz Mołoń Karolina Stępień Patrycja Kielar Bela Vasileva Bonka Lozanska Dessislava Staneva Penyo Ivanov Monika Kula-Maximenko Eliza Molestak Marek Tchórzewski George Miloshev Milena Georgieva |
| author_facet | Mateusz Mołoń Karolina Stępień Patrycja Kielar Bela Vasileva Bonka Lozanska Dessislava Staneva Penyo Ivanov Monika Kula-Maximenko Eliza Molestak Marek Tchórzewski George Miloshev Milena Georgieva |
| author_sort | Mateusz Mołoń |
| collection | DOAJ |
| description | Ageing is accompanied by dramatic changes in chromatin structure organization and genome function. Two essential components of chromatin, the linker histone Hho1p and actin-related protein 4 (Arp4p), have been shown to physically interact in <i>Saccharomyces cerevisiae</i> cells, thus maintaining chromatin dynamics and function, as well as genome stability and cellular morphology. Disrupting this interaction has been proven to influence the stability of the yeast genome and the way cells respond to stress during chronological ageing. It has also been proven that the abrogated interaction between these two chromatin proteins elicited premature ageing phenotypes. Alterations in chromatin compaction have also been associated with replicative ageing, though the main players are not well recognized. Based on this knowledge, here, we examine how the interaction between Hho1p and Arp4p impacts the ageing of mitotically active yeast cells. For this purpose, two sets of strains were used—haploids (WT(n), <i>arp4</i>, <i>hho1Δ</i> and <i>arp4 hho1Δ</i>) and their heterozygous diploid counterparts (WT(2n), <i>ARP4</i>/<i>arp4</i>, <i>HHO1</i>/<i>hho1Δ</i> and <i>ARP4 HHO1</i>/<i>arp4 hho1Δ</i>)—for the performance of extensive morphological and physiological analyses during replicative ageing. These analyses included a comparative examination of the yeast cells’ chromatin structure, proliferative and reproductive potential, and resilience to stress, as well as polysome profiles and chemical composition. The results demonstrated that the haploid chromatin mutants <i>arp4</i> and <i>arp4 hho1Δ</i> demonstrated a significant reduction in replicative and total lifespan. These findings lead to the conclusion that the importance of a healthy interaction between Arp4p and Hho1p in replicative ageing is significant. This is proof of the concomitant importance of Hho1p and Arp4p in chronological and replicative ageing. |
| first_indexed | 2024-03-10T01:55:56Z |
| format | Article |
| id | doaj.art-5d077816eba94c979617fd4836672869 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T01:55:56Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-5d077816eba94c979617fd48366728692023-11-23T12:56:24ZengMDPI AGCells2073-44092022-09-011117275410.3390/cells11172754Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative AgeingMateusz Mołoń0Karolina Stępień1Patrycja Kielar2Bela Vasileva3Bonka Lozanska4Dessislava Staneva5Penyo Ivanov6Monika Kula-Maximenko7Eliza Molestak8Marek Tchórzewski9George Miloshev10Milena Georgieva11Department of Biochemistry and Cell Biology, Institute of Biology and Biotechnology, University of Rzeszow, 35-601 Rzeszow, PolandDepartment of Biochemistry and Cell Biology, Institute of Biology and Biotechnology, University of Rzeszow, 35-601 Rzeszow, PolandDepartment of Biochemistry and Cell Biology, Institute of Biology and Biotechnology, University of Rzeszow, 35-601 Rzeszow, PolandLaboratory of Yeast Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, Bulgarian Academy of Sciences, 1123 Sofia, BulgariaLaboratory of Yeast Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, Bulgarian Academy of Sciences, 1123 Sofia, BulgariaLaboratory of Yeast Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, Bulgarian Academy of Sciences, 1123 Sofia, BulgariaLaboratory of Yeast Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, Bulgarian Academy of Sciences, 1123 Sofia, BulgariaThe Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 30-239 Kraków, PolandDepartment of Molecular Biology, Maria Curie-Skłodowska University, 20-033 Lublin, PolandDepartment of Molecular Biology, Maria Curie-Skłodowska University, 20-033 Lublin, PolandLaboratory of Yeast Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, Bulgarian Academy of Sciences, 1123 Sofia, BulgariaLaboratory of Yeast Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, Bulgarian Academy of Sciences, 1123 Sofia, BulgariaAgeing is accompanied by dramatic changes in chromatin structure organization and genome function. Two essential components of chromatin, the linker histone Hho1p and actin-related protein 4 (Arp4p), have been shown to physically interact in <i>Saccharomyces cerevisiae</i> cells, thus maintaining chromatin dynamics and function, as well as genome stability and cellular morphology. Disrupting this interaction has been proven to influence the stability of the yeast genome and the way cells respond to stress during chronological ageing. It has also been proven that the abrogated interaction between these two chromatin proteins elicited premature ageing phenotypes. Alterations in chromatin compaction have also been associated with replicative ageing, though the main players are not well recognized. Based on this knowledge, here, we examine how the interaction between Hho1p and Arp4p impacts the ageing of mitotically active yeast cells. For this purpose, two sets of strains were used—haploids (WT(n), <i>arp4</i>, <i>hho1Δ</i> and <i>arp4 hho1Δ</i>) and their heterozygous diploid counterparts (WT(2n), <i>ARP4</i>/<i>arp4</i>, <i>HHO1</i>/<i>hho1Δ</i> and <i>ARP4 HHO1</i>/<i>arp4 hho1Δ</i>)—for the performance of extensive morphological and physiological analyses during replicative ageing. These analyses included a comparative examination of the yeast cells’ chromatin structure, proliferative and reproductive potential, and resilience to stress, as well as polysome profiles and chemical composition. The results demonstrated that the haploid chromatin mutants <i>arp4</i> and <i>arp4 hho1Δ</i> demonstrated a significant reduction in replicative and total lifespan. These findings lead to the conclusion that the importance of a healthy interaction between Arp4p and Hho1p in replicative ageing is significant. This is proof of the concomitant importance of Hho1p and Arp4p in chronological and replicative ageing.https://www.mdpi.com/2073-4409/11/17/2754ageingactin-related protein 4Arp4plinker histoneHho1preplicative lifespan |
| spellingShingle | Mateusz Mołoń Karolina Stępień Patrycja Kielar Bela Vasileva Bonka Lozanska Dessislava Staneva Penyo Ivanov Monika Kula-Maximenko Eliza Molestak Marek Tchórzewski George Miloshev Milena Georgieva Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing Cells ageing actin-related protein 4 Arp4p linker histone Hho1p replicative lifespan |
| title | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
| title_full | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
| title_fullStr | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
| title_full_unstemmed | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
| title_short | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
| title_sort | actin related protein 4 and linker histone sustain yeast replicative ageing |
| topic | ageing actin-related protein 4 Arp4p linker histone Hho1p replicative lifespan |
| url | https://www.mdpi.com/2073-4409/11/17/2754 |
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