Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9

Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9

Genome editing via the CRISPR/Cas9 RNA-guided nuclease system has opened up exciting possibilities for genetic analysis. However, technical challenges associated with homology-directed repair have proven to be roadblocks for producing changes in the absence of unwanted, secondary mutations commonly...

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Bibliographic Details
Main Authors: Abigail M. Lamb, Elizabeth A. Walker, Patricia J. Wittkopp
Format: Article
Language:English
Published: Taylor & Francis Group 2017-01-01
Series:Fly
Subjects:
crispr
genome editing
homology directed repair
non-model species
point mutation
single-nucleotide
transgenic
Online Access:http://dx.doi.org/10.1080/19336934.2016.1220463

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