Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species <i>Solanum microdontum</i> and <i>Solanum pampasense</i>
Wild potato species continue to be a rich source of genes for resistance to late blight in potato breeding. Whilst many dominant resistance genes from such sources have been characterised and used in breeding, quantitative resistance also offers potential for breeding when the loci underlying the re...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/7/732 |
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| author | Fergus Meade Ronald Hutten Silke Wagener Vanessa Prigge Emmet Dalton Hanne Grethe Kirk Denis Griffin Dan Milbourne |
| author_facet | Fergus Meade Ronald Hutten Silke Wagener Vanessa Prigge Emmet Dalton Hanne Grethe Kirk Denis Griffin Dan Milbourne |
| author_sort | Fergus Meade |
| collection | DOAJ |
| description | Wild potato species continue to be a rich source of genes for resistance to late blight in potato breeding. Whilst many dominant resistance genes from such sources have been characterised and used in breeding, quantitative resistance also offers potential for breeding when the loci underlying the resistance can be identified and tagged using molecular markers. In this study, F<sub>1</sub> populations were created from crosses between blight susceptible parents and lines exhibiting strong partial resistance to late blight derived from the South American wild species <i>Solanum microdontum</i> and <i>Solanum pampasense</i>. Both populations exhibited continuous variation for resistance to late blight over multiple field-testing seasons. High density genetic maps were created using single nucleotide polymorphism (SNP) markers, enabling mapping of quantitative trait loci (QTLs) for late blight resistance that were consistently expressed over multiple years in both populations. In the population created with the <i>S. microdontum</i> source, QTLs for resistance consistently expressed over three years and explaining a large portion (21–47%) of the phenotypic variation were found on chromosomes 5 and 6, and a further resistance QTL on chromosome 10, apparently related to foliar development, was discovered in 2016 only. In the population created with the <i>S. pampasense</i> source, QTLs for resistance were found in over two years on chromosomes 11 and 12. For all loci detected consistently across years, the QTLs span known R gene clusters and so they likely represent novel late blight resistance genes. Simple genetic models following the effect of the presence or absence of SNPs associated with consistently effective loci in both populations demonstrated that marker assisted selection (MAS) strategies to introgress and pyramid these loci have potential in resistance breeding strategies. |
| first_indexed | 2024-03-10T18:46:24Z |
| format | Article |
| id | doaj.art-5d10a25861a24ccc8e2ed69f55984054 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T18:46:24Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-5d10a25861a24ccc8e2ed69f559840542023-11-20T05:29:03ZengMDPI AGGenes2073-44252020-06-0111773210.3390/genes11070732Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species <i>Solanum microdontum</i> and <i>Solanum pampasense</i>Fergus Meade0Ronald Hutten1Silke Wagener2Vanessa Prigge3Emmet Dalton4Hanne Grethe Kirk5Denis Griffin6Dan Milbourne7Teagasc, Crop Science Department, Oak Park, R93 XE12 Carlow, IrelandWageningen University & Research (WUR), 6708 PB Wageningen, The NetherlandsSaKa Pflanzenzucht GmbH & Co., 22761 Hamburg, GermanySaKa Pflanzenzucht GmbH & Co., 22761 Hamburg, GermanyAardevo, 8308 PB Nagele, The NetherlandsDanespo, 7323 Give, DenmarkTeagasc, Crop Science Department, Oak Park, R93 XE12 Carlow, IrelandTeagasc, Crop Science Department, Oak Park, R93 XE12 Carlow, IrelandWild potato species continue to be a rich source of genes for resistance to late blight in potato breeding. Whilst many dominant resistance genes from such sources have been characterised and used in breeding, quantitative resistance also offers potential for breeding when the loci underlying the resistance can be identified and tagged using molecular markers. In this study, F<sub>1</sub> populations were created from crosses between blight susceptible parents and lines exhibiting strong partial resistance to late blight derived from the South American wild species <i>Solanum microdontum</i> and <i>Solanum pampasense</i>. Both populations exhibited continuous variation for resistance to late blight over multiple field-testing seasons. High density genetic maps were created using single nucleotide polymorphism (SNP) markers, enabling mapping of quantitative trait loci (QTLs) for late blight resistance that were consistently expressed over multiple years in both populations. In the population created with the <i>S. microdontum</i> source, QTLs for resistance consistently expressed over three years and explaining a large portion (21–47%) of the phenotypic variation were found on chromosomes 5 and 6, and a further resistance QTL on chromosome 10, apparently related to foliar development, was discovered in 2016 only. In the population created with the <i>S. pampasense</i> source, QTLs for resistance were found in over two years on chromosomes 11 and 12. For all loci detected consistently across years, the QTLs span known R gene clusters and so they likely represent novel late blight resistance genes. Simple genetic models following the effect of the presence or absence of SNPs associated with consistently effective loci in both populations demonstrated that marker assisted selection (MAS) strategies to introgress and pyramid these loci have potential in resistance breeding strategies.https://www.mdpi.com/2073-4425/11/7/732potatobreedinglate blight<i>Phytophthora infestans</i>marker-assisted selectionQTL |
| spellingShingle | Fergus Meade Ronald Hutten Silke Wagener Vanessa Prigge Emmet Dalton Hanne Grethe Kirk Denis Griffin Dan Milbourne Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species <i>Solanum microdontum</i> and <i>Solanum pampasense</i> Genes potato breeding late blight <i>Phytophthora infestans</i> marker-assisted selection QTL |
| title | Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species <i>Solanum microdontum</i> and <i>Solanum pampasense</i> |
| title_full | Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species <i>Solanum microdontum</i> and <i>Solanum pampasense</i> |
| title_fullStr | Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species <i>Solanum microdontum</i> and <i>Solanum pampasense</i> |
| title_full_unstemmed | Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species <i>Solanum microdontum</i> and <i>Solanum pampasense</i> |
| title_short | Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species <i>Solanum microdontum</i> and <i>Solanum pampasense</i> |
| title_sort | detection of novel qtls for late blight resistance derived from the wild potato species i solanum microdontum i and i solanum pampasense i |
| topic | potato breeding late blight <i>Phytophthora infestans</i> marker-assisted selection QTL |
| url | https://www.mdpi.com/2073-4425/11/7/732 |
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