Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary Fiber
This study aims to determine the impact of different GDF9 genotypes on feed intake, nutrient digestibility, and nitrogen balance in Kacang goats by examining various metabolic processes. Twenty-nine Kacang goats were genotyped using PCR-RFLP DNA at position g.3855A/C of the GDF9 gene in exon 2. A 2...
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| Format: | Article |
| Language: | English |
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IPB University
2024-03-01
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| Series: | Tropical Animal Science Journal |
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| Online Access: | https://journal.ipb.ac.id/index.php/tasj/article/view/48454 |
| _version_ | 1797271180713918464 |
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| author | A. Irawan T. Hartatik S. Bintara A. Astuti Kustantinah |
| author_facet | A. Irawan T. Hartatik S. Bintara A. Astuti Kustantinah |
| author_sort | A. Irawan |
| collection | DOAJ |
| description |
This study aims to determine the impact of different GDF9 genotypes on feed intake, nutrient digestibility, and nitrogen balance in Kacang goats by examining various metabolic processes. Twenty-nine Kacang goats were genotyped using PCR-RFLP DNA at position g.3855A/C of the GDF9 gene in exon 2. A 2 × 2 factorial design with two factors, namely, diet type (diets 1 and 2) and genotype (homozygote AA and heterozygote AC), was adopted. Diet 1 group comprised 11 goats with the AA genotype and 4 goats with the AC genotype, and the diet 2 group consisted of 9 goats with the AA genotype and 5 goats with the AC genotype. Both diets had the same protein content (iso-protein) and consisted of free-choice Napier grass (Pennisetum purpureum). Diet 1 had an additional 400 g of concentrate containing 11.25% crude protein (CP) and 55.86% total digestible nutrients (TDN). Diet 2 was a total mixed ration (TMR) containing 12.46% crude protein and 67.92% TDN. Results demonstrated a significant interaction (p<0.05) effect between diet and genotype on crude protein (CP) digestibility, neutral detergent fiber (NDF) digestibility, fecal nitrogen (N feces), urinary nitrogen (N urine), nitrogen retention (%), and blood urea nitrogen (BUN). However, no significant interaction effect was observed on the consumption of dry matter (DM), CP, crude fiber (CF), nitrogen free extract (NFE), and NDF and the digestibility of DM, CF, and NFE. TMR resulted in a 9% higher consumption of NDF, higher nutrient digestibility, improved nitrogen balance, average daily gain (ADG), and elevated glucose levels compared with diet 1. Kacang goats with the AC genotype exhibited better CP digestibility and increased nitrogen intake than those with the AA genotype. In conclusion, the GDF9 genotype influences the nutrient digestibility in Kacang goats, and those with the AC genotype utilize feed nutrients more efficiently than those with the AA genotype. The AC genotype resulting from the GDF9 gene mutation at position g.3855A>C can be used for genomic marker selection of high-quality Kacang goats in nutrient digestibility.
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| first_indexed | 2024-03-07T13:58:05Z |
| format | Article |
| id | doaj.art-5d1162ee416a491eaadc54c9e561128a |
| institution | Directory Open Access Journal |
| issn | 2615-787X 2615-790X |
| language | English |
| last_indexed | 2024-03-07T13:58:05Z |
| publishDate | 2024-03-01 |
| publisher | IPB University |
| record_format | Article |
| series | Tropical Animal Science Journal |
| spelling | doaj.art-5d1162ee416a491eaadc54c9e561128a2024-03-07T06:23:24ZengIPB UniversityTropical Animal Science Journal2615-787X2615-790X2024-03-0147110.5398/tasj.2024.47.1.33Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary FiberA. Irawan0T. Hartatik 1S. Bintara2A. Astuti3Kustantinah4Universitas Gadjah MadaLaboratory of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah MadaLaboratory of Animal Physiology and Reproduction, Animal Science and Industry, Faculty of Animal Science, Universitas Gadjah MadaLaboratory of Animal Nutrition, Animal Science and Industry, Faculty of Animal Science, Universitas Gadjah MadaLaboratory of Animal Nutrition, Animal Science and Industry, Faculty of Animal Science, Universitas Gadjah Mada This study aims to determine the impact of different GDF9 genotypes on feed intake, nutrient digestibility, and nitrogen balance in Kacang goats by examining various metabolic processes. Twenty-nine Kacang goats were genotyped using PCR-RFLP DNA at position g.3855A/C of the GDF9 gene in exon 2. A 2 × 2 factorial design with two factors, namely, diet type (diets 1 and 2) and genotype (homozygote AA and heterozygote AC), was adopted. Diet 1 group comprised 11 goats with the AA genotype and 4 goats with the AC genotype, and the diet 2 group consisted of 9 goats with the AA genotype and 5 goats with the AC genotype. Both diets had the same protein content (iso-protein) and consisted of free-choice Napier grass (Pennisetum purpureum). Diet 1 had an additional 400 g of concentrate containing 11.25% crude protein (CP) and 55.86% total digestible nutrients (TDN). Diet 2 was a total mixed ration (TMR) containing 12.46% crude protein and 67.92% TDN. Results demonstrated a significant interaction (p<0.05) effect between diet and genotype on crude protein (CP) digestibility, neutral detergent fiber (NDF) digestibility, fecal nitrogen (N feces), urinary nitrogen (N urine), nitrogen retention (%), and blood urea nitrogen (BUN). However, no significant interaction effect was observed on the consumption of dry matter (DM), CP, crude fiber (CF), nitrogen free extract (NFE), and NDF and the digestibility of DM, CF, and NFE. TMR resulted in a 9% higher consumption of NDF, higher nutrient digestibility, improved nitrogen balance, average daily gain (ADG), and elevated glucose levels compared with diet 1. Kacang goats with the AC genotype exhibited better CP digestibility and increased nitrogen intake than those with the AA genotype. In conclusion, the GDF9 genotype influences the nutrient digestibility in Kacang goats, and those with the AC genotype utilize feed nutrients more efficiently than those with the AA genotype. The AC genotype resulting from the GDF9 gene mutation at position g.3855A>C can be used for genomic marker selection of high-quality Kacang goats in nutrient digestibility. https://journal.ipb.ac.id/index.php/tasj/article/view/48454GDF9genotype interactionKacang goatsnutrient digestibility |
| spellingShingle | A. Irawan T. Hartatik S. Bintara A. Astuti Kustantinah Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary Fiber Tropical Animal Science Journal GDF9 genotype interaction Kacang goats nutrient digestibility |
| title | Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary Fiber |
| title_full | Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary Fiber |
| title_fullStr | Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary Fiber |
| title_full_unstemmed | Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary Fiber |
| title_short | Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary Fiber |
| title_sort | nutrient digestibility n balance performance and blood parameters of kacang goats differing in gdf9 genotype fed different sources of dietary fiber |
| topic | GDF9 genotype interaction Kacang goats nutrient digestibility |
| url | https://journal.ipb.ac.id/index.php/tasj/article/view/48454 |
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