Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of <i>Euphorbia hirta</i> Linn. Extracts
This study aimed to screen the anticancer and antioxidant potential and antimicrobial activity of methanol, petroleum ether, chloroform, ethyl acetate, butanol of <i>Euphorbia hirta</i> Linn. extracts (EH-Me, EH-PE, EH-Ch, EH-EA and EH-Bu, respectively). The results of 2,2-diphenyl-1-pyc...
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MDPI AG
2020-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/23/8408 |
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| author | Ngan Tran Minh Nguyen Khanh PB Le Nhi Nguyen Quan Tran Ly Le |
| author_facet | Ngan Tran Minh Nguyen Khanh PB Le Nhi Nguyen Quan Tran Ly Le |
| author_sort | Ngan Tran |
| collection | DOAJ |
| description | This study aimed to screen the anticancer and antioxidant potential and antimicrobial activity of methanol, petroleum ether, chloroform, ethyl acetate, butanol of <i>Euphorbia hirta</i> Linn. extracts (EH-Me, EH-PE, EH-Ch, EH-EA and EH-Bu, respectively). The results of 2,2-diphenyl-1-pycrylhydrazyl (DPPH) radical scavenging assay and lipid peroxidation inhibition assay showed that EH-EA was the strongest antioxidant (IC<sub>50</sub> = 10.33 ± 0.01 µg/mL; IC<sub>50</sub> = 1.48 ± 0.12 µg/mL, respectively) compared to all other extracts. In the antimicrobial activity of the extracts against eight strains of Gram-positive and Gram-negative bacteria using the agar disc diffusion method, we found the EH-EA to be the best antimicrobial agent. Anticancer activities of those extracts were examined by sulforhodamine B (SRB) in vitro cytotoxicity assay on two cancer cell lines, including lung cancer cells NCI-H460 and liver cancer cells Hep G2. EH-EA at concentration of 100 μg/mL has significant inhibitory activity the growth of lung cancer cells NCI-H460 and liver cancer cells Hep G2 compared to all other extracts. Our results suggest that <i>E. hirta</i> Linn. extracts possess significant biological activities, including antimicrobial, antioxidant, and moderate anticancer properties. Our results show that this plant could be a good source for natural antioxidants and a possible pharmaceutical supplement. Among five analyzed extracts, EH-EA extract has the strongest activities, and should be used to determine phytochemicals and mechanisms of these activities. |
| first_indexed | 2024-03-10T14:33:00Z |
| format | Article |
| id | doaj.art-5d167d9c71c441119ec6880811587622 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T14:33:00Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-5d167d9c71c441119ec68808115876222023-11-20T22:23:05ZengMDPI AGApplied Sciences2076-34172020-11-011023840810.3390/app10238408Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of <i>Euphorbia hirta</i> Linn. ExtractsNgan Tran0Minh Nguyen1Khanh PB Le2Nhi Nguyen3Quan Tran4Ly Le5School of Biotechnology, International University–Vietnam National University, Ho Chi Minh City 70000, VietnamResearch Center of Ginseng and Medicinal Materials Ho Chi Minh City 2, 41 Dinh Tien Hoang Street, District 1, Ho Chi Minh City 70000, VietnamSchool of Biotechnology, International University–Vietnam National University, Ho Chi Minh City 70000, VietnamFaculty of Chemistry, University of Science–Vietnam National University, Ho Chi Minh City 70000, VietnamFaculty of Chemistry, University of Science–Vietnam National University, Ho Chi Minh City 70000, VietnamSchool of Biotechnology, International University–Vietnam National University, Ho Chi Minh City 70000, VietnamThis study aimed to screen the anticancer and antioxidant potential and antimicrobial activity of methanol, petroleum ether, chloroform, ethyl acetate, butanol of <i>Euphorbia hirta</i> Linn. extracts (EH-Me, EH-PE, EH-Ch, EH-EA and EH-Bu, respectively). The results of 2,2-diphenyl-1-pycrylhydrazyl (DPPH) radical scavenging assay and lipid peroxidation inhibition assay showed that EH-EA was the strongest antioxidant (IC<sub>50</sub> = 10.33 ± 0.01 µg/mL; IC<sub>50</sub> = 1.48 ± 0.12 µg/mL, respectively) compared to all other extracts. In the antimicrobial activity of the extracts against eight strains of Gram-positive and Gram-negative bacteria using the agar disc diffusion method, we found the EH-EA to be the best antimicrobial agent. Anticancer activities of those extracts were examined by sulforhodamine B (SRB) in vitro cytotoxicity assay on two cancer cell lines, including lung cancer cells NCI-H460 and liver cancer cells Hep G2. EH-EA at concentration of 100 μg/mL has significant inhibitory activity the growth of lung cancer cells NCI-H460 and liver cancer cells Hep G2 compared to all other extracts. Our results suggest that <i>E. hirta</i> Linn. extracts possess significant biological activities, including antimicrobial, antioxidant, and moderate anticancer properties. Our results show that this plant could be a good source for natural antioxidants and a possible pharmaceutical supplement. Among five analyzed extracts, EH-EA extract has the strongest activities, and should be used to determine phytochemicals and mechanisms of these activities.https://www.mdpi.com/2076-3417/10/23/8408<i>Euphorbia hirta</i> Linn.antibacterial activityantioxidant activityanticancer activity |
| spellingShingle | Ngan Tran Minh Nguyen Khanh PB Le Nhi Nguyen Quan Tran Ly Le Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of <i>Euphorbia hirta</i> Linn. Extracts Applied Sciences <i>Euphorbia hirta</i> Linn. antibacterial activity antioxidant activity anticancer activity |
| title | Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of <i>Euphorbia hirta</i> Linn. Extracts |
| title_full | Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of <i>Euphorbia hirta</i> Linn. Extracts |
| title_fullStr | Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of <i>Euphorbia hirta</i> Linn. Extracts |
| title_full_unstemmed | Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of <i>Euphorbia hirta</i> Linn. Extracts |
| title_short | Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of <i>Euphorbia hirta</i> Linn. Extracts |
| title_sort | screening of antibacterial activity antioxidant activity and anticancer activity of i euphorbia hirta i linn extracts |
| topic | <i>Euphorbia hirta</i> Linn. antibacterial activity antioxidant activity anticancer activity |
| url | https://www.mdpi.com/2076-3417/10/23/8408 |
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