Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis
This study summarizes the synthesis of AgNPs using nanocurcumin as a reducing and stabilizing agent (CurAgNPs). The stability of CurAgNPs after 12 months of storage and their antimicrobial activity against four bacteria causing mastitis in cows were investigated. Ultraviolet — visible (UV–vis) spect...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2023-01-01
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| Series: | Materials Research Express |
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| Online Access: | https://doi.org/10.1088/2053-1591/acdb36 |
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| author | Quoc Bao Vo-Van Anh Quang Dao Van-Phuong Nguyen Nguyen Duc Danh Thanh Hai Duong Le Thi Kim Anh |
| author_facet | Quoc Bao Vo-Van Anh Quang Dao Van-Phuong Nguyen Nguyen Duc Danh Thanh Hai Duong Le Thi Kim Anh |
| author_sort | Quoc Bao Vo-Van |
| collection | DOAJ |
| description | This study summarizes the synthesis of AgNPs using nanocurcumin as a reducing and stabilizing agent (CurAgNPs). The stability of CurAgNPs after 12 months of storage and their antimicrobial activity against four bacteria causing mastitis in cows were investigated. Ultraviolet — visible (UV–vis) spectroscopy of the dark brownish-red stabilized CurAgNPs solution decating the surface plasmon resonance peak of the sample was observed at 438 nm. Images of the spherical CurAgNPs were obtained using transmission electron microscopy (TEM), which showed a mean particle size distribution of 15–58 nm, with a mean size of 32 nm. The influence of CurAgNPs on four microorganisms that cause mastitis in cows, Streptococcus agalactiae ( S. agalactiae ), Staphylococcus aureus ( S. aureus ), Pseudomonas aeruginosa ( P. aeruginosa ), and Eschericia coli ( E. coli ), was studied, and the minimum inhibitory concentration (MIC) was from 1.6 to 6.25 ppm. From this, the MBC of CurAgNPs were observed at 3.15 ppm for S. agalactiae , and 6.25 ppm for S. aureus , P. aeruginosa and E. coli , respectively. The formulated homogeneous gel containing 100 ppm CurAgNPs, 1.5% carboxymethyl cellulose (CMC), and distilled water was developed with a viscosity of 141 ± 7.55 cP, pH of 6.72 ± 0.11, and homogenized. The zeta potential of CurAgNPs gel after 6 months of storage is almost constant. In addition, the improved CurAgNPs gel demonstrated significant antimicrobial activity compared with tetracycline at a concentration of 100 ppm. |
| first_indexed | 2024-03-12T15:37:16Z |
| format | Article |
| id | doaj.art-5d1cd1c76afc4cedb9cf8ae4382b3f87 |
| institution | Directory Open Access Journal |
| issn | 2053-1591 |
| language | English |
| last_indexed | 2024-03-12T15:37:16Z |
| publishDate | 2023-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Research Express |
| spelling | doaj.art-5d1cd1c76afc4cedb9cf8ae4382b3f872023-08-09T16:08:20ZengIOP PublishingMaterials Research Express2053-15912023-01-0110606500410.1088/2053-1591/acdb36Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitisQuoc Bao Vo-Van0https://orcid.org/0009-0002-4302-8292Anh Quang Dao1https://orcid.org/0000-0002-8208-2956Van-Phuong Nguyen2https://orcid.org/0000-0001-9614-4508Nguyen Duc Danh3Thanh Hai Duong4Le Thi Kim Anh5University of Agriculture and Forestry, Hue University , 102 Phung Hung St., Hue, VietnamThe Faculty of Environmental and Natural Sciences, School of Engineering and Technology, Duy Tan University , Danang 550000, VietnamUniversity of Science and Technology of Hanoi , VAST, 18 Hoang Quoc Viet St., Hanoi, VietnamCenter for Dairy Cow Hoof Care in Lam Dong, VietnamUniversity of Agriculture and Forestry, Hue University , 102 Phung Hung St., Hue, VietnamUniversity of Agriculture and Forestry, Hue University , 102 Phung Hung St., Hue, VietnamThis study summarizes the synthesis of AgNPs using nanocurcumin as a reducing and stabilizing agent (CurAgNPs). The stability of CurAgNPs after 12 months of storage and their antimicrobial activity against four bacteria causing mastitis in cows were investigated. Ultraviolet — visible (UV–vis) spectroscopy of the dark brownish-red stabilized CurAgNPs solution decating the surface plasmon resonance peak of the sample was observed at 438 nm. Images of the spherical CurAgNPs were obtained using transmission electron microscopy (TEM), which showed a mean particle size distribution of 15–58 nm, with a mean size of 32 nm. The influence of CurAgNPs on four microorganisms that cause mastitis in cows, Streptococcus agalactiae ( S. agalactiae ), Staphylococcus aureus ( S. aureus ), Pseudomonas aeruginosa ( P. aeruginosa ), and Eschericia coli ( E. coli ), was studied, and the minimum inhibitory concentration (MIC) was from 1.6 to 6.25 ppm. From this, the MBC of CurAgNPs were observed at 3.15 ppm for S. agalactiae , and 6.25 ppm for S. aureus , P. aeruginosa and E. coli , respectively. The formulated homogeneous gel containing 100 ppm CurAgNPs, 1.5% carboxymethyl cellulose (CMC), and distilled water was developed with a viscosity of 141 ± 7.55 cP, pH of 6.72 ± 0.11, and homogenized. The zeta potential of CurAgNPs gel after 6 months of storage is almost constant. In addition, the improved CurAgNPs gel demonstrated significant antimicrobial activity compared with tetracycline at a concentration of 100 ppm.https://doi.org/10.1088/2053-1591/acdb36antibacterial activityantibacterial gelcharacterizationgel formulationsilver nanoparticles |
| spellingShingle | Quoc Bao Vo-Van Anh Quang Dao Van-Phuong Nguyen Nguyen Duc Danh Thanh Hai Duong Le Thi Kim Anh Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis Materials Research Express antibacterial activity antibacterial gel characterization gel formulation silver nanoparticles |
| title | Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis |
| title_full | Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis |
| title_fullStr | Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis |
| title_full_unstemmed | Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis |
| title_short | Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis |
| title_sort | antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis |
| topic | antibacterial activity antibacterial gel characterization gel formulation silver nanoparticles |
| url | https://doi.org/10.1088/2053-1591/acdb36 |
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