Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity Preservation
Background: Northern Italy has an enormous heritage of hop biodiversity that need to be exploited and studied. The preservation and valorization through the characterization of the existent biodiversity is a primary goal of the European Green Deal 2023–2030. The aim of this study was to acquire info...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/12/11/5751 |
| _version_ | 1797493974435364864 |
|---|---|
| author | Margherita Rodolfi Matteo Marieschi Benedetta Chiancone Tommaso Ganino |
| author_facet | Margherita Rodolfi Matteo Marieschi Benedetta Chiancone Tommaso Ganino |
| author_sort | Margherita Rodolfi |
| collection | DOAJ |
| description | Background: Northern Italy has an enormous heritage of hop biodiversity that need to be exploited and studied. The preservation and valorization through the characterization of the existent biodiversity is a primary goal of the European Green Deal 2023–2030. The aim of this study was to acquire information on the biodiversity of Italian wild hops. Methods: Genetic characterization of sixty accessions was done resorting to Single Sequence Repeated (SSR) markers. Phytochemical characterization of wild hops was achieved using: (i) high-performance liquid chromatography with ultraviolet detection for bitter acids quantification, (ii) steam distillation for essential oils quantification and (iii) Gas Chromatography-Mass Spectrometry for the determination of the aromatic profile. Results: The eight SSR primers showed high Polymorphic Information Content (PIC), especially HlGA23. α-Acids reached values between 0 and 4.125. The essential oils analysis highlighted variability within the studied population, with some accessions characterized by important spicy fraction, and others by fruity and floral notes. Conclusions: The present study allowed the characterization of Italian wild hops and demonstrated an interesting biodiversity. Part of this biodiversity have been shown to be potentially suitable for use in brewing. Moreover, several genotypes could be used in breeding programs to obtain new more sustainable varieties. |
| first_indexed | 2024-03-10T01:27:41Z |
| format | Article |
| id | doaj.art-5d1e723d0bab4b12b5ddbcd9bda977bf |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T01:27:41Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-5d1e723d0bab4b12b5ddbcd9bda977bf2023-11-23T13:47:18ZengMDPI AGApplied Sciences2076-34172022-06-011211575110.3390/app12115751Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity PreservationMargherita Rodolfi0Matteo Marieschi1Benedetta Chiancone2Tommaso Ganino3Department of Food and Drug, University of Parma, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, ItalyDepartment of Food and Drug, University of Parma, 43124 Parma, ItalyDepartment of Food and Drug, University of Parma, 43124 Parma, ItalyBackground: Northern Italy has an enormous heritage of hop biodiversity that need to be exploited and studied. The preservation and valorization through the characterization of the existent biodiversity is a primary goal of the European Green Deal 2023–2030. The aim of this study was to acquire information on the biodiversity of Italian wild hops. Methods: Genetic characterization of sixty accessions was done resorting to Single Sequence Repeated (SSR) markers. Phytochemical characterization of wild hops was achieved using: (i) high-performance liquid chromatography with ultraviolet detection for bitter acids quantification, (ii) steam distillation for essential oils quantification and (iii) Gas Chromatography-Mass Spectrometry for the determination of the aromatic profile. Results: The eight SSR primers showed high Polymorphic Information Content (PIC), especially HlGA23. α-Acids reached values between 0 and 4.125. The essential oils analysis highlighted variability within the studied population, with some accessions characterized by important spicy fraction, and others by fruity and floral notes. Conclusions: The present study allowed the characterization of Italian wild hops and demonstrated an interesting biodiversity. Part of this biodiversity have been shown to be potentially suitable for use in brewing. Moreover, several genotypes could be used in breeding programs to obtain new more sustainable varieties.https://www.mdpi.com/2076-3417/12/11/5751SSRUPGMAcluster analysisbitter acidsHPLC-UVGC-MS |
| spellingShingle | Margherita Rodolfi Matteo Marieschi Benedetta Chiancone Tommaso Ganino Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity Preservation Applied Sciences SSR UPGMA cluster analysis bitter acids HPLC-UV GC-MS |
| title | Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity Preservation |
| title_full | Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity Preservation |
| title_fullStr | Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity Preservation |
| title_full_unstemmed | Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity Preservation |
| title_short | Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity Preservation |
| title_sort | assessment of the genetic and phytochemical variability of italian wild hop a route to biodiversity preservation |
| topic | SSR UPGMA cluster analysis bitter acids HPLC-UV GC-MS |
| url | https://www.mdpi.com/2076-3417/12/11/5751 |
| work_keys_str_mv | AT margheritarodolfi assessmentofthegeneticandphytochemicalvariabilityofitalianwildhoparoutetobiodiversitypreservation AT matteomarieschi assessmentofthegeneticandphytochemicalvariabilityofitalianwildhoparoutetobiodiversitypreservation AT benedettachiancone assessmentofthegeneticandphytochemicalvariabilityofitalianwildhoparoutetobiodiversitypreservation AT tommasoganino assessmentofthegeneticandphytochemicalvariabilityofitalianwildhoparoutetobiodiversitypreservation |