Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle <i>Cerambyx welensii</i>
Actinomycetes constitute a large group of Gram-positive bacteria present in different habitats. One of these habitats involves the association of these bacteria with insects. In this work, we have studied twenty-four actinomycetes strains isolated from the intestinal tract and feces from larvae of t...
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MDPI AG
2020-12-01
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| author | Ramón I. Santamaría Ana Martínez-Carrasco Ricardo Sánchez de la Nieta Luis M. Torres-Vila Raúl Bonal Jesús Martín Rubén Tormo Fernando Reyes Olga Genilloud Margarita Díaz |
| author_facet | Ramón I. Santamaría Ana Martínez-Carrasco Ricardo Sánchez de la Nieta Luis M. Torres-Vila Raúl Bonal Jesús Martín Rubén Tormo Fernando Reyes Olga Genilloud Margarita Díaz |
| author_sort | Ramón I. Santamaría |
| collection | DOAJ |
| description | Actinomycetes constitute a large group of Gram-positive bacteria present in different habitats. One of these habitats involves the association of these bacteria with insects. In this work, we have studied twenty-four actinomycetes strains isolated from the intestinal tract and feces from larvae of the xylophagous coleopteran <i>Cerambyx welensii</i> and have shown that seventeen strains present hydrolytic activity of some of the following substrates: cellulose, hemicellulose, starch and proteins. Fourteen of the isolates produce antimicrobial molecules against the Gram-positive bacteria <i>Micrococcus luteus</i>. Analysis of seven strains led us to identify the production of a wide number of compounds including streptanoate, alpiniamide A, alteramides A and B, coproporphyrin III, deferoxamine, demethylenenocardamine, dihydropicromycin, nocardamine, picromycin, surugamides A, B, C, D and E, tirandamycins A and B, and valinomycin. A significant number of other compounds, whose molecular formulae are not included in the Dictionary of Natural Products (DNP), were also present in the extracts analyzed, which opens up the possibility of identifying new active antibiotics. Molecular identification of ten of the isolated bacteria determined that six of them belong to the genus <i>Streptomyces</i>, two of them are included in the genus <i>Amycolatopsis</i> and two in the genus <i>Nocardiopsis.</i> |
| first_indexed | 2024-03-10T14:01:00Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:01:00Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5d20cdc0405f48b0987b29484f0794412023-11-21T01:06:00ZengMDPI AGMicroorganisms2076-26072020-12-01812201310.3390/microorganisms8122013Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle <i>Cerambyx welensii</i>Ramón I. Santamaría0Ana Martínez-Carrasco1Ricardo Sánchez de la Nieta2Luis M. Torres-Vila3Raúl Bonal4Jesús Martín5Rubén Tormo6Fernando Reyes7Olga Genilloud8Margarita Díaz9Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca, C/Zacarías González 2, 37007 Salamanca, SpainDepartamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca, C/Zacarías González 2, 37007 Salamanca, SpainDepartamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca, C/Zacarías González 2, 37007 Salamanca, SpainServicio de Sanidad Vegetal, Consejería de Agricultura DRPyT, Junta de Extremadura, Avda. Luis Ramallo s/n, 06800 Badajoz, SpainForest Research Group, INDEHESA, Escuela de Ingeniería Forestal, Universidad de Extremadura, Avda. Virgen del Puerto 2, 10600 Cáceres, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Granada, SpainDepartamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca, C/Zacarías González 2, 37007 Salamanca, SpainActinomycetes constitute a large group of Gram-positive bacteria present in different habitats. One of these habitats involves the association of these bacteria with insects. In this work, we have studied twenty-four actinomycetes strains isolated from the intestinal tract and feces from larvae of the xylophagous coleopteran <i>Cerambyx welensii</i> and have shown that seventeen strains present hydrolytic activity of some of the following substrates: cellulose, hemicellulose, starch and proteins. Fourteen of the isolates produce antimicrobial molecules against the Gram-positive bacteria <i>Micrococcus luteus</i>. Analysis of seven strains led us to identify the production of a wide number of compounds including streptanoate, alpiniamide A, alteramides A and B, coproporphyrin III, deferoxamine, demethylenenocardamine, dihydropicromycin, nocardamine, picromycin, surugamides A, B, C, D and E, tirandamycins A and B, and valinomycin. A significant number of other compounds, whose molecular formulae are not included in the Dictionary of Natural Products (DNP), were also present in the extracts analyzed, which opens up the possibility of identifying new active antibiotics. Molecular identification of ten of the isolated bacteria determined that six of them belong to the genus <i>Streptomyces</i>, two of them are included in the genus <i>Amycolatopsis</i> and two in the genus <i>Nocardiopsis.</i>https://www.mdpi.com/2076-2607/8/12/2013actinomycetes<i>Streptomyces</i><i>Amycolatopsis</i><i>Nocardiopsis</i><i>Cerambyx</i>antibiotic production |
| spellingShingle | Ramón I. Santamaría Ana Martínez-Carrasco Ricardo Sánchez de la Nieta Luis M. Torres-Vila Raúl Bonal Jesús Martín Rubén Tormo Fernando Reyes Olga Genilloud Margarita Díaz Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle <i>Cerambyx welensii</i> Microorganisms actinomycetes <i>Streptomyces</i> <i>Amycolatopsis</i> <i>Nocardiopsis</i> <i>Cerambyx</i> antibiotic production |
| title | Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle <i>Cerambyx welensii</i> |
| title_full | Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle <i>Cerambyx welensii</i> |
| title_fullStr | Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle <i>Cerambyx welensii</i> |
| title_full_unstemmed | Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle <i>Cerambyx welensii</i> |
| title_short | Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle <i>Cerambyx welensii</i> |
| title_sort | characterization of actinomycetes strains isolated from the intestinal tract and feces of the larvae of the longhorn beetle i cerambyx welensii i |
| topic | actinomycetes <i>Streptomyces</i> <i>Amycolatopsis</i> <i>Nocardiopsis</i> <i>Cerambyx</i> antibiotic production |
| url | https://www.mdpi.com/2076-2607/8/12/2013 |
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