Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin
Background and Objectives: Vitamin B2 (riboflavin) is a water-soluble vitamin and yellow. The aim of this study is to identify ‎strains of Bacillus isolated from soil do with the ability to produce riboflavin by PCR. Materials and Methods: Soil samples was mild randomly during 2 consecutive...
| Main Authors: | , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Alborz University of Medical Sciencs
2017-11-01
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| Series: | Bihdād |
| Subjects: | |
| Online Access: | http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-190&slc_lang=en&sid=1 |
| Summary: | Background and Objectives: Vitamin B2 (riboflavin) is a water-soluble vitamin and yellow. The aim of this study is to identify ‎strains of Bacillus isolated from soil do with the ability to produce riboflavin by PCR.
Materials and Methods: Soil samples was mild randomly during 2 consecutive days the air. Sampling was performed of areas far from the sun ‎and from 3 to 10 cm soil depth. The genus Bacillus were identified to species level ‎using standard methods. Also isolates were approved identified using the PCR method. To study the ‎riboflavin production of synthetic culture media were used and HPLC techniques.
Result: PCR using gene 16SrRNA test results showed that 32 strains obtained from a strain of Bacillus subtilis ‎and 10 were 2 strain of Bacillus megaterium. The isolated strains of Bacillus megaterium PTCC 1250 with ‎standard strains were positive of riboflavin production in synthetic medium and HPLC.
‎Conclusions: Bacillus subtilis bacteria living in the soil among the most important role in the production of ‎riboflavin and riboflavin-producing strains to confirm the use of molecular methods is necessary. |
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| ISSN: | 1735-6679 |