Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin
Background and Objectives: Vitamin B2 (riboflavin) is a water-soluble vitamin and yellow. The aim of this study is to identify ‎strains of Bacillus isolated from soil do with the ability to produce riboflavin by PCR. Materials and Methods: Soil samples was mild randomly during 2 consecutive...
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| Format: | Article |
| Language: | fas |
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Alborz University of Medical Sciencs
2017-11-01
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| Series: | Bihdād |
| Subjects: | |
| Online Access: | http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-190&slc_lang=en&sid=1 |
| _version_ | 1818310387598098432 |
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| author | Niloofar Chaharmahali Abass Akhavan Sepahi Mohammad Reza Awwadi |
| author_facet | Niloofar Chaharmahali Abass Akhavan Sepahi Mohammad Reza Awwadi |
| author_sort | Niloofar Chaharmahali |
| collection | DOAJ |
| description | Background and Objectives: Vitamin B2 (riboflavin) is a water-soluble vitamin and yellow. The aim of this study is to identify ‎strains of Bacillus isolated from soil do with the ability to produce riboflavin by PCR.
Materials and Methods: Soil samples was mild randomly during 2 consecutive days the air. Sampling was performed of areas far from the sun ‎and from 3 to 10 cm soil depth. The genus Bacillus were identified to species level ‎using standard methods. Also isolates were approved identified using the PCR method. To study the ‎riboflavin production of synthetic culture media were used and HPLC techniques.
Result: PCR using gene 16SrRNA test results showed that 32 strains obtained from a strain of Bacillus subtilis ‎and 10 were 2 strain of Bacillus megaterium. The isolated strains of Bacillus megaterium PTCC 1250 with ‎standard strains were positive of riboflavin production in synthetic medium and HPLC.
‎Conclusions: Bacillus subtilis bacteria living in the soil among the most important role in the production of ‎riboflavin and riboflavin-producing strains to confirm the use of molecular methods is necessary. |
| first_indexed | 2024-12-13T07:45:15Z |
| format | Article |
| id | doaj.art-5d233273866e4e7b82ac8e3f854185d8 |
| institution | Directory Open Access Journal |
| issn | 1735-6679 |
| language | fas |
| last_indexed | 2024-12-13T07:45:15Z |
| publishDate | 2017-11-01 |
| publisher | Alborz University of Medical Sciencs |
| record_format | Article |
| series | Bihdād |
| spelling | doaj.art-5d233273866e4e7b82ac8e3f854185d82022-12-21T23:54:51ZfasAlborz University of Medical SciencsBihdād1735-66792017-11-0164283290Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of RiboflavinNiloofar Chaharmahali0Abass Akhavan Sepahi1Mohammad Reza Awwadi2 Department of Microbiology, School of Biology Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran Department of Microbiology, School of Biology Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran Department of Nanotechnology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran Background and Objectives: Vitamin B2 (riboflavin) is a water-soluble vitamin and yellow. The aim of this study is to identify ‎strains of Bacillus isolated from soil do with the ability to produce riboflavin by PCR. Materials and Methods: Soil samples was mild randomly during 2 consecutive days the air. Sampling was performed of areas far from the sun ‎and from 3 to 10 cm soil depth. The genus Bacillus were identified to species level ‎using standard methods. Also isolates were approved identified using the PCR method. To study the ‎riboflavin production of synthetic culture media were used and HPLC techniques. Result: PCR using gene 16SrRNA test results showed that 32 strains obtained from a strain of Bacillus subtilis ‎and 10 were 2 strain of Bacillus megaterium. The isolated strains of Bacillus megaterium PTCC 1250 with ‎standard strains were positive of riboflavin production in synthetic medium and HPLC. ‎Conclusions: Bacillus subtilis bacteria living in the soil among the most important role in the production of ‎riboflavin and riboflavin-producing strains to confirm the use of molecular methods is necessary.http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-190&slc_lang=en&sid=1Bacillus species Riboflavin 16SrRNA |
| spellingShingle | Niloofar Chaharmahali Abass Akhavan Sepahi Mohammad Reza Awwadi Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin Bihdād Bacillus species Riboflavin 16SrRNA |
| title | Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin |
| title_full | Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin |
| title_fullStr | Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin |
| title_full_unstemmed | Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin |
| title_short | Comparison of the Native Bacillus subtillis and Bacillus Megaterium Strains Isolated from Soil in Production of Riboflavin |
| title_sort | comparison of the native bacillus subtillis and bacillus megaterium strains isolated from soil in production of riboflavin |
| topic | Bacillus species Riboflavin 16SrRNA |
| url | http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-190&slc_lang=en&sid=1 |
| work_keys_str_mv | AT niloofarchaharmahali comparisonofthenativebacillussubtillisandbacillusmegateriumstrainsisolatedfromsoilinproductionofriboflavin AT abassakhavansepahi comparisonofthenativebacillussubtillisandbacillusmegateriumstrainsisolatedfromsoilinproductionofriboflavin AT mohammadrezaawwadi comparisonofthenativebacillussubtillisandbacillusmegateriumstrainsisolatedfromsoilinproductionofriboflavin |