Molecular characterization of Blastocystis sp. isolates from human, cattle and sheep by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene
<em>Blastocystis</em> sp. is an enteric unicellular, anaerobic, protist that could be isolated from humans and many other farm and wild animals, in addition to rodents, reptiles and insects. This study aimed to characterize <em>Blastocystis </em>isolated from fecal samples of...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2016-06-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://vetmedmosul.com/article_119164_a4ceb89ed7f600b60089e810fede62e8.pdf |
| _version_ | 1818844886901719040 |
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| author | Abdul Aziz Al-Ani H. S. Albakri |
| author_facet | Abdul Aziz Al-Ani H. S. Albakri |
| author_sort | Abdul Aziz Al-Ani |
| collection | DOAJ |
| description | <em>Blastocystis</em> sp. is an enteric unicellular, anaerobic, protist that could be isolated from humans and many other farm and wild animals, in addition to rodents, reptiles and insects. This study aimed to characterize <em>Blastocystis </em>isolated from fecal samples of human, cattle and sheep using restriction fragment length polymorphism (RFLP) analysis. Pre-determined samples were selected from human, cattle and sheep that showed to be positive by polymerase chain reaction (PCR) targeting the small subunit ribosomal RNA gene (SSU ‑ rRNA) then examined by RFLP analysis using HinfI enzyme to determine the molecular patterns. The results of RFLP analysis revealed the presence of three different patterns of human isolates which were in difference with the other three patterns of cattle isolates. However, sheep isolates showed only one pattern. This study shows that RFLP analysis is a simple and rapid technique that could be used to characterize and differentiate Blastocystis sp. in humans and animals. |
| first_indexed | 2024-12-19T05:20:54Z |
| format | Article |
| id | doaj.art-5d23d8a0afed447e920646250dc89413 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-19T05:20:54Z |
| publishDate | 2016-06-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-5d23d8a0afed447e920646250dc894132022-12-21T20:34:31ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552016-06-013011710.33899/ijvs.2016.119164119164Molecular characterization of Blastocystis sp. isolates from human, cattle and sheep by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA geneAbdul Aziz Al-AniH. S. Albakri<em>Blastocystis</em> sp. is an enteric unicellular, anaerobic, protist that could be isolated from humans and many other farm and wild animals, in addition to rodents, reptiles and insects. This study aimed to characterize <em>Blastocystis </em>isolated from fecal samples of human, cattle and sheep using restriction fragment length polymorphism (RFLP) analysis. Pre-determined samples were selected from human, cattle and sheep that showed to be positive by polymerase chain reaction (PCR) targeting the small subunit ribosomal RNA gene (SSU ‑ rRNA) then examined by RFLP analysis using HinfI enzyme to determine the molecular patterns. The results of RFLP analysis revealed the presence of three different patterns of human isolates which were in difference with the other three patterns of cattle isolates. However, sheep isolates showed only one pattern. This study shows that RFLP analysis is a simple and rapid technique that could be used to characterize and differentiate Blastocystis sp. in humans and animals.https://vetmedmosul.com/article_119164_a4ceb89ed7f600b60089e810fede62e8.pdfblastocystis sprestriction fragment length polymorphismsmall subunit ribosomal rna generrna |
| spellingShingle | Abdul Aziz Al-Ani H. S. Albakri Molecular characterization of Blastocystis sp. isolates from human, cattle and sheep by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene Iraqi Journal of Veterinary Sciences blastocystis sp restriction fragment length polymorphism small subunit ribosomal rna gene rrna |
| title | Molecular characterization of Blastocystis sp. isolates from human, cattle and sheep by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene |
| title_full | Molecular characterization of Blastocystis sp. isolates from human, cattle and sheep by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene |
| title_fullStr | Molecular characterization of Blastocystis sp. isolates from human, cattle and sheep by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene |
| title_full_unstemmed | Molecular characterization of Blastocystis sp. isolates from human, cattle and sheep by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene |
| title_short | Molecular characterization of Blastocystis sp. isolates from human, cattle and sheep by restriction fragment length polymorphism (RFLP) analysis of the small subunit ribosomal RNA gene |
| title_sort | molecular characterization of blastocystis sp isolates from human cattle and sheep by restriction fragment length polymorphism rflp analysis of the small subunit ribosomal rna gene |
| topic | blastocystis sp restriction fragment length polymorphism small subunit ribosomal rna gene rrna |
| url | https://vetmedmosul.com/article_119164_a4ceb89ed7f600b60089e810fede62e8.pdf |
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