High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis
Metabolomics is an effective strategy to explore the molecular mechanism of herbal medicine. Epimedium, a traditional Chinese herb from the Epimedium brevicornu Maxim., has a therapeutic effect on osteoporosis (OP), however the molecular mechanism of the anti-OP effect is uncle\ar. Therefore, we inv...
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Frontiers Media S.A.
2020-08-01
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.01318/full |
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| author | Jun-feng Zhao Jian-yu Xu Yi-er Xu Shui-lin Chen Yan-xing Guo Quan-yang Gao Gui-cai Sun |
| author_facet | Jun-feng Zhao Jian-yu Xu Yi-er Xu Shui-lin Chen Yan-xing Guo Quan-yang Gao Gui-cai Sun |
| author_sort | Jun-feng Zhao |
| collection | DOAJ |
| description | Metabolomics is an effective strategy to explore the molecular mechanism of herbal medicine. Epimedium, a traditional Chinese herb from the Epimedium brevicornu Maxim., has a therapeutic effect on osteoporosis (OP), however the molecular mechanism of the anti-OP effect is uncle\ar. Therefore, we investigated the pharmacological effect and action mechanism of ethanol extract of epimedium (Ext-epi) onOP rat model. The serum of OP rats was analyzed utilized UPLC-Q-TOF/MS metabolomics, and the potential biomarkers were screened and identified using multivariate data analysis systems and network databases. To further appraise the influence of Ext-epi on biological markers and metabolic pathways, and reveal the potential mechanism of Ext-epi on OP treatment. The results showed that 46 potential biomarkers were screened out and after intervention with Ext-epi extracts solution, 16 potential biomarkers were significantly recalled. Further pathway experiments showed that key pathway analysis include sarachidonic acid metabolism, glycerolphospholipid metabolism as potential targets which is related with the efficacy of Ext-epi protect against OP. These results explain the correlation between metabolites and molecular mechanisms, which is of great significance for understanding the intervention of Ext-epi on OP. In short, based on UPLC-Q-TOF/MS metabolomics may provide effective strategies for understanding the pathogenesis of diseases and evaluating the intervention effect of natural products. |
| first_indexed | 2024-12-10T06:28:04Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-10T06:28:04Z |
| publishDate | 2020-08-01 |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-5d292dcdd702497d9cb6869f7f4297812022-12-22T01:59:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-08-011110.3389/fphar.2020.01318563706High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against OsteoporosisJun-feng Zhao0Jian-yu Xu1Yi-er Xu2Shui-lin Chen3Yan-xing Guo4Quan-yang Gao5Gui-cai Sun6The Manual Orthopaedics, Henan Province Luoyang Orthopedic Thraumatological Hospital (Henan Provincal Orthopedic Hospital), Luoyang, ChinaThe Tumor Hospital of Harbin Medical University, The Department of Radiation Oncology, The Affiliated Tumour Hospital of Harbin Medical University, Harbin, ChinaThe Research & Development Center of Harbin Pharmaceutical Group, The Laboratory of Pharmacology Quality Inspection & Pilotscale Experiment Workshop, Harbin, ChinaThe Department of Orthopaedics, The Fourth Hospital Attached to Nanchang University, Nanchang, ChinaThe Manual Orthopaedics, Henan Province Luoyang Orthopedic Thraumatological Hospital (Henan Provincal Orthopedic Hospital), Luoyang, ChinaThe Manual Orthopaedics, Henan Province Luoyang Orthopedic Thraumatological Hospital (Henan Provincal Orthopedic Hospital), Luoyang, ChinaThe First Affiliated Hospital of Nanchang University, Orthopaedics, Nanchang University, Nanchang, ChinaMetabolomics is an effective strategy to explore the molecular mechanism of herbal medicine. Epimedium, a traditional Chinese herb from the Epimedium brevicornu Maxim., has a therapeutic effect on osteoporosis (OP), however the molecular mechanism of the anti-OP effect is uncle\ar. Therefore, we investigated the pharmacological effect and action mechanism of ethanol extract of epimedium (Ext-epi) onOP rat model. The serum of OP rats was analyzed utilized UPLC-Q-TOF/MS metabolomics, and the potential biomarkers were screened and identified using multivariate data analysis systems and network databases. To further appraise the influence of Ext-epi on biological markers and metabolic pathways, and reveal the potential mechanism of Ext-epi on OP treatment. The results showed that 46 potential biomarkers were screened out and after intervention with Ext-epi extracts solution, 16 potential biomarkers were significantly recalled. Further pathway experiments showed that key pathway analysis include sarachidonic acid metabolism, glycerolphospholipid metabolism as potential targets which is related with the efficacy of Ext-epi protect against OP. These results explain the correlation between metabolites and molecular mechanisms, which is of great significance for understanding the intervention of Ext-epi on OP. In short, based on UPLC-Q-TOF/MS metabolomics may provide effective strategies for understanding the pathogenesis of diseases and evaluating the intervention effect of natural products.https://www.frontiersin.org/article/10.3389/fphar.2020.01318/fullmetabolomicsbiomarkermetabolismpathwaymolecular mechanismosteoporosis |
| spellingShingle | Jun-feng Zhao Jian-yu Xu Yi-er Xu Shui-lin Chen Yan-xing Guo Quan-yang Gao Gui-cai Sun High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis Frontiers in Pharmacology metabolomics biomarker metabolism pathway molecular mechanism osteoporosis |
| title | High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis |
| title_full | High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis |
| title_fullStr | High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis |
| title_full_unstemmed | High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis |
| title_short | High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis |
| title_sort | high throughput metabolomics method for discovering metabolic biomarkers and pathways to reveal effects and molecular mechanism of ethanol extract from epimedium against osteoporosis |
| topic | metabolomics biomarker metabolism pathway molecular mechanism osteoporosis |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.01318/full |
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