Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration
Research on the regenerative capacity of the neonatal heart could open new avenues for the treatment of myocardial infarction (MI). However, the mechanism of cardiac regeneration remains unclear. In the present study, we constructed a mouse model of heart regeneration and then performed transcripto...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2023-01-01
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| Series: | Biomolecules & Biomedicine |
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| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/7770 |
| _version_ | 1797261365963915264 |
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| author | Liu Liu Tongtong Yang Qiqi Jiang Jiateng Sun Lingfeng Gu Sibo Wang Yafei Li Bingrui Chen Di Zhao Rui Sun Qiming Wang Hao Wang Liansheng Wang |
| author_facet | Liu Liu Tongtong Yang Qiqi Jiang Jiateng Sun Lingfeng Gu Sibo Wang Yafei Li Bingrui Chen Di Zhao Rui Sun Qiming Wang Hao Wang Liansheng Wang |
| author_sort | Liu Liu |
| collection | DOAJ |
| description |
Research on the regenerative capacity of the neonatal heart could open new avenues for the treatment of myocardial infarction (MI). However, the mechanism of cardiac regeneration remains unclear. In the present study, we constructed a mouse model of heart regeneration and then performed transcriptomic and proteomic analyses on them. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and Gene Set Enrichment Analysis (GSEA) of differentially expressed genes (DEGs) were conducted. Western blot (WB) and qPCR analyses were used to validate the hub genes expression. As a result, gene expression at the mRNA level and protein level is not the same. We identified 3186 DEGs and 42 differentially expressed proteins (DEPs). Through functional analysis of DEGs and DEPs, we speculate that biological processes such as ubiquitination, cell cycle, and oxygen metabolism are involved in heart regeneration. Integrated transcriptomic and proteomic analysis identified 19 hub genes and Ankrd1, Gpx3, and Trim72 were screened out as potential regulators of cardiac regeneration through further expression verification. In conclusion, we combined transcriptomic and proteomic analyses to characterize the molecular features during heart regeneration in neonatal mice. Finally, Ankrd1, Gpx3, and Trim72 were identified as potential targets for heart regeneration therapy.
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| first_indexed | 2024-04-24T23:40:04Z |
| format | Article |
| id | doaj.art-5d295b871fe74713bc45a4842724fb27 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:40:04Z |
| publishDate | 2023-01-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-5d295b871fe74713bc45a4842724fb272024-03-15T13:22:46ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2023-01-0123110.17305/bjbms.2022.7770Integrated transcriptomic and proteomic analysis reveals potential targets for heart regenerationLiu Liu0https://orcid.org/0000-0003-3560-7585Tongtong Yang1Qiqi Jiang2Jiateng Sun3Lingfeng Gu4Sibo Wang5Yafei Li6Bingrui Chen7Di Zhao8Rui Sun9Qiming Wang10Hao Wang11https://orcid.org/0000-0002-1053-5303Liansheng Wang12https://orcid.org/0000-0001-8538-6560Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Research on the regenerative capacity of the neonatal heart could open new avenues for the treatment of myocardial infarction (MI). However, the mechanism of cardiac regeneration remains unclear. In the present study, we constructed a mouse model of heart regeneration and then performed transcriptomic and proteomic analyses on them. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and Gene Set Enrichment Analysis (GSEA) of differentially expressed genes (DEGs) were conducted. Western blot (WB) and qPCR analyses were used to validate the hub genes expression. As a result, gene expression at the mRNA level and protein level is not the same. We identified 3186 DEGs and 42 differentially expressed proteins (DEPs). Through functional analysis of DEGs and DEPs, we speculate that biological processes such as ubiquitination, cell cycle, and oxygen metabolism are involved in heart regeneration. Integrated transcriptomic and proteomic analysis identified 19 hub genes and Ankrd1, Gpx3, and Trim72 were screened out as potential regulators of cardiac regeneration through further expression verification. In conclusion, we combined transcriptomic and proteomic analyses to characterize the molecular features during heart regeneration in neonatal mice. Finally, Ankrd1, Gpx3, and Trim72 were identified as potential targets for heart regeneration therapy. https://www.bjbms.org/ojs/index.php/bjbms/article/view/7770Proteomictranscriptomicheart regenerationmyocardial infarction |
| spellingShingle | Liu Liu Tongtong Yang Qiqi Jiang Jiateng Sun Lingfeng Gu Sibo Wang Yafei Li Bingrui Chen Di Zhao Rui Sun Qiming Wang Hao Wang Liansheng Wang Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration Biomolecules & Biomedicine Proteomic transcriptomic heart regeneration myocardial infarction |
| title | Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration |
| title_full | Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration |
| title_fullStr | Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration |
| title_full_unstemmed | Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration |
| title_short | Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration |
| title_sort | integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration |
| topic | Proteomic transcriptomic heart regeneration myocardial infarction |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/7770 |
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