Study on the expression of TRIM7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic value
Abstract Background The early diagnosis of sepsis is beneficial to put forward a reasonable clinical treatment plan as soon as possible. This study was to explore the expression of Tripartite Motif 7 (TRIM7) in peripheral blood mononuclear cells (PBMCs) of patients with sepsis and its diagnostic val...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-022-07874-6 |
| _version_ | 1811189391291318272 |
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| author | Mingfeng Lu Aiwen Ma Jianwei Liu Wenzhen Zhou Peng Cao Tao Chu Lu Fan |
| author_facet | Mingfeng Lu Aiwen Ma Jianwei Liu Wenzhen Zhou Peng Cao Tao Chu Lu Fan |
| author_sort | Mingfeng Lu |
| collection | DOAJ |
| description | Abstract Background The early diagnosis of sepsis is beneficial to put forward a reasonable clinical treatment plan as soon as possible. This study was to explore the expression of Tripartite Motif 7 (TRIM7) in peripheral blood mononuclear cells (PBMCs) of patients with sepsis and its diagnostic value. Methods This is a cross-sectional study. A total of 69 patients with infectious diseases were enrolled in the emergency room. They were divided into the sepsis group (34 cases) and the non-sepsis infection group (35 cases). There were 25 healthy subjects who were selected as the control group. The expression of TRIM7 in PBMCs was observed by immunofluorescence staining. The correlation between the expression of TRIM7 mRNA and acute physiology and chronic health evaluation II (APACHE II) score, sequential organ failure assessment (SOFA) score, white blood cell (WBC), C-reactive protein (CRP), procalcitonin (PCT), tumor necrosis factor (TNF)-α and interleukin (IL)-6 was discussed. The receiver operating characteristic (ROC) curve was utilized for evaluating the value of TRIM7 expression for the early diagnosis of sepsis. Results The fluorescence intensity representing the expression level of TRIM7 in PBMCs of patients in the sepsis group was the lowest among three groups. The TRIM7 mRNA expression in PBMCs of the sepsis group was greatly decreased in comparison with that of the non-sepsis infection group and control group (P < 0.05). Spearman correlation analysis indicated that TRIM7 mRNA expression was negatively correlated with APACHE II score, SOFA score, WBC, CRP, PCT, TNF-α and IL-6. ROC curve analysis revealed that the area under curve (AUC) of TRIM7 mRNA expression in PBMCs for the diagnosis of sepsis was 0.798, with a 95% confidence interval of 0.691- 0.905, a sensitivity of 73.5%, and a specificity of 77.1%. Conclusion The expression of TRIM7 in PBMCs of patients with sepsis is significantly down-regulated, which has certain clinical value for early diagnosis of sepsis. |
| first_indexed | 2024-04-11T14:35:07Z |
| format | Article |
| id | doaj.art-5d2982ea644e4997a396f1548815aabb |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-11T14:35:07Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-5d2982ea644e4997a396f1548815aabb2022-12-22T04:18:21ZengBMCBMC Infectious Diseases1471-23342022-11-012211810.1186/s12879-022-07874-6Study on the expression of TRIM7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic valueMingfeng Lu0Aiwen Ma1Jianwei Liu2Wenzhen Zhou3Peng Cao4Tao Chu5Lu Fan6Department of Emergency, Clinical Medical College, Yangzhou UniversityDepartment of Emergency, Clinical Medical College, Yangzhou UniversityDepartment of Emergency, Clinical Medical College, Yangzhou UniversityDepartment of Emergency, Clinical Medical College, Yangzhou UniversityDepartment of Emergency, Clinical Medical College, Yangzhou UniversityDepartment of Emergency, Clinical Medical College, Yangzhou UniversityDepartment of Emergency, Clinical Medical College, Yangzhou UniversityAbstract Background The early diagnosis of sepsis is beneficial to put forward a reasonable clinical treatment plan as soon as possible. This study was to explore the expression of Tripartite Motif 7 (TRIM7) in peripheral blood mononuclear cells (PBMCs) of patients with sepsis and its diagnostic value. Methods This is a cross-sectional study. A total of 69 patients with infectious diseases were enrolled in the emergency room. They were divided into the sepsis group (34 cases) and the non-sepsis infection group (35 cases). There were 25 healthy subjects who were selected as the control group. The expression of TRIM7 in PBMCs was observed by immunofluorescence staining. The correlation between the expression of TRIM7 mRNA and acute physiology and chronic health evaluation II (APACHE II) score, sequential organ failure assessment (SOFA) score, white blood cell (WBC), C-reactive protein (CRP), procalcitonin (PCT), tumor necrosis factor (TNF)-α and interleukin (IL)-6 was discussed. The receiver operating characteristic (ROC) curve was utilized for evaluating the value of TRIM7 expression for the early diagnosis of sepsis. Results The fluorescence intensity representing the expression level of TRIM7 in PBMCs of patients in the sepsis group was the lowest among three groups. The TRIM7 mRNA expression in PBMCs of the sepsis group was greatly decreased in comparison with that of the non-sepsis infection group and control group (P < 0.05). Spearman correlation analysis indicated that TRIM7 mRNA expression was negatively correlated with APACHE II score, SOFA score, WBC, CRP, PCT, TNF-α and IL-6. ROC curve analysis revealed that the area under curve (AUC) of TRIM7 mRNA expression in PBMCs for the diagnosis of sepsis was 0.798, with a 95% confidence interval of 0.691- 0.905, a sensitivity of 73.5%, and a specificity of 77.1%. Conclusion The expression of TRIM7 in PBMCs of patients with sepsis is significantly down-regulated, which has certain clinical value for early diagnosis of sepsis.https://doi.org/10.1186/s12879-022-07874-6TRIM7PBMCsSepsisDiagnosis |
| spellingShingle | Mingfeng Lu Aiwen Ma Jianwei Liu Wenzhen Zhou Peng Cao Tao Chu Lu Fan Study on the expression of TRIM7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic value BMC Infectious Diseases TRIM7 PBMCs Sepsis Diagnosis |
| title | Study on the expression of TRIM7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic value |
| title_full | Study on the expression of TRIM7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic value |
| title_fullStr | Study on the expression of TRIM7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic value |
| title_full_unstemmed | Study on the expression of TRIM7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic value |
| title_short | Study on the expression of TRIM7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic value |
| title_sort | study on the expression of trim7 in peripheral blood mononuclear cells of patients with sepsis and its early diagnostic value |
| topic | TRIM7 PBMCs Sepsis Diagnosis |
| url | https://doi.org/10.1186/s12879-022-07874-6 |
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