Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method
Ochratoxin A (OTA) is a type of mycotoxin generated from the metabolism of Aspergillus and Penicillium, and is extremely toxic to humans, livestock, and poultry. However, traditional assays for the detection of OTA are expensive and complicated. Other than OTA aptamer, OTA itself at high concentrati...
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Frontiers Media S.A.
2018-04-01
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Online Access: | http://journal.frontiersin.org/article/10.3389/fchem.2018.00094/full |
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author | Boshi Liu Renliang Huang Renliang Huang Yanjun Yu Rongxin Su Rongxin Su Wei Qi Wei Qi Zhimin He |
author_facet | Boshi Liu Renliang Huang Renliang Huang Yanjun Yu Rongxin Su Rongxin Su Wei Qi Wei Qi Zhimin He |
author_sort | Boshi Liu |
collection | DOAJ |
description | Ochratoxin A (OTA) is a type of mycotoxin generated from the metabolism of Aspergillus and Penicillium, and is extremely toxic to humans, livestock, and poultry. However, traditional assays for the detection of OTA are expensive and complicated. Other than OTA aptamer, OTA itself at high concentration can also adsorb on the surface of gold nanoparticles (AuNPs), and further inhibit AuNPs salt aggregation. We herein report a new OTA assay by applying the localized surface plasmon resonance effect of AuNPs and their aggregates. The result obtained from only one single linear calibration curve is not reliable, and so we developed a “double calibration curve” method to address this issue and widen the OTA detection range. A number of other analytes were also examined, and the structural properties of analytes that bind with the AuNPs were further discussed. We found that various considerations must be taken into account in the detection of these analytes when applying AuNP aggregation-based methods due to their different binding strengths. |
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language | English |
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spelling | doaj.art-5d29badcc1d6430d8876a4bffa3a01892022-12-22T03:21:24ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462018-04-01610.3389/fchem.2018.00094351096Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve MethodBoshi Liu0Renliang Huang1Renliang Huang2Yanjun Yu3Rongxin Su4Rongxin Su5Wei Qi6Wei Qi7Zhimin He8State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaSchool of Environmental Science and Engineering, Tianjin University, Tianjin, ChinaState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaState Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaOchratoxin A (OTA) is a type of mycotoxin generated from the metabolism of Aspergillus and Penicillium, and is extremely toxic to humans, livestock, and poultry. However, traditional assays for the detection of OTA are expensive and complicated. Other than OTA aptamer, OTA itself at high concentration can also adsorb on the surface of gold nanoparticles (AuNPs), and further inhibit AuNPs salt aggregation. We herein report a new OTA assay by applying the localized surface plasmon resonance effect of AuNPs and their aggregates. The result obtained from only one single linear calibration curve is not reliable, and so we developed a “double calibration curve” method to address this issue and widen the OTA detection range. A number of other analytes were also examined, and the structural properties of analytes that bind with the AuNPs were further discussed. We found that various considerations must be taken into account in the detection of these analytes when applying AuNP aggregation-based methods due to their different binding strengths.http://journal.frontiersin.org/article/10.3389/fchem.2018.00094/fullaptamerLSPRochratoxin AAuNP aggregationcolorimetric detection |
spellingShingle | Boshi Liu Renliang Huang Renliang Huang Yanjun Yu Rongxin Su Rongxin Su Wei Qi Wei Qi Zhimin He Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method Frontiers in Chemistry aptamer LSPR ochratoxin A AuNP aggregation colorimetric detection |
title | Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method |
title_full | Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method |
title_fullStr | Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method |
title_full_unstemmed | Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method |
title_short | Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method |
title_sort | gold nanoparticle aptamer based lspr sensing of ochratoxin a at a widened detection range by double calibration curve method |
topic | aptamer LSPR ochratoxin A AuNP aggregation colorimetric detection |
url | http://journal.frontiersin.org/article/10.3389/fchem.2018.00094/full |
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