Comparative Genomics Analysis of Protease Genes in Bacillus sp.
【Objective】The study aims to explore the relationship between the protease-producing capacity of Bacillus and the protease genes carried by the bacteria and analyze the important enzyme genes that affect the protease-producing capacity of Bacillus.【Method】The protease-producing strain was screened b...
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| Format: | Article |
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Guangdong Academy of Agricultural Sciences
2022-05-01
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| Series: | Guangdong nongye kexue |
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| Online Access: | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202205013 |
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| author | Zhongxin JIA Jianan ZHAO Fang JI Xue WANG Gang LI Chengmin WANG Jianhua QIN |
| author_facet | Zhongxin JIA Jianan ZHAO Fang JI Xue WANG Gang LI Chengmin WANG Jianhua QIN |
| author_sort | Zhongxin JIA |
| collection | DOAJ |
| description | 【Objective】The study aims to explore the relationship between the protease-producing capacity of Bacillus and the protease genes carried by the bacteria and analyze the important enzyme genes that affect the protease-producing capacity of Bacillus.【Method】The protease-producing strain was screened by using the selective medium. The protease activity of the strains was determined by the Folin method. The protease genes carried on the chromosomes of the strains were obtained and classified by sequencing the whole genome of 15 strains of Bacillus. The transcription level of protease gene mRNA was measured by the real-time fluorescent quantitative PCR method. Through comparative genomics and Bacterial Genome-Wide Association Analysis (BGWAS), the reasons that affect the ability of bacteria to produce protease were explored.【Result】According to the protease genes carried on the chromosomes of the strains, they could be divided into 7 groups of genotypes (G1-G7). Comparative genomics and BGWAS found that the number of protease genes carried on bacterial chromosomes was directly related to the protease production capacity, and the protease production capacity of strains lacking aprX and aprE was significantly reduced. The difference in the protease production capacity of Bacillus was found to be related to the expression level of aprX by further studying the mRNA level of the gene.【Conclusion】There is a correlation between the protease gene carried on the chromosome of the strain and the protease production capacity of the strain. The important enzyme gene that affects the protease production capacity of Bacillus is aprX. |
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| format | Article |
| id | doaj.art-5d2dd2b83bdc4a43b28493b26b2b820f |
| institution | Directory Open Access Journal |
| issn | 1004-874X |
| language | English |
| last_indexed | 2024-03-13T05:07:53Z |
| publishDate | 2022-05-01 |
| publisher | Guangdong Academy of Agricultural Sciences |
| record_format | Article |
| series | Guangdong nongye kexue |
| spelling | doaj.art-5d2dd2b83bdc4a43b28493b26b2b820f2023-06-16T06:41:48ZengGuangdong Academy of Agricultural SciencesGuangdong nongye kexue1004-874X2022-05-0149511011710.16768/j.issn.1004-874X.2022.05.013202205013Comparative Genomics Analysis of Protease Genes in Bacillus sp.Zhongxin JIA0Jianan ZHAO1Fang JI2Xue WANG3Gang LI4Chengmin WANG5Jianhua QIN6College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, ChinaInstitute of Zoology, Guangdong Academy of Science / Guangdong Key Laboratory of Animal Conservation and Resource Utilization / Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou 510260, ChinaInstitute of Zoology, Guangdong Academy of Science / Guangdong Key Laboratory of Animal Conservation and Resource Utilization / Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou 510260, ChinaInstitute of Zoology, Guangdong Academy of Science / Guangdong Key Laboratory of Animal Conservation and Resource Utilization / Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou 510260, ChinaInstitute of Zoology, Guangdong Academy of Science / Guangdong Key Laboratory of Animal Conservation and Resource Utilization / Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou 510260, ChinaInstitute of Zoology, Guangdong Academy of Science / Guangdong Key Laboratory of Animal Conservation and Resource Utilization / Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou 510260, ChinaCollege of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China【Objective】The study aims to explore the relationship between the protease-producing capacity of Bacillus and the protease genes carried by the bacteria and analyze the important enzyme genes that affect the protease-producing capacity of Bacillus.【Method】The protease-producing strain was screened by using the selective medium. The protease activity of the strains was determined by the Folin method. The protease genes carried on the chromosomes of the strains were obtained and classified by sequencing the whole genome of 15 strains of Bacillus. The transcription level of protease gene mRNA was measured by the real-time fluorescent quantitative PCR method. Through comparative genomics and Bacterial Genome-Wide Association Analysis (BGWAS), the reasons that affect the ability of bacteria to produce protease were explored.【Result】According to the protease genes carried on the chromosomes of the strains, they could be divided into 7 groups of genotypes (G1-G7). Comparative genomics and BGWAS found that the number of protease genes carried on bacterial chromosomes was directly related to the protease production capacity, and the protease production capacity of strains lacking aprX and aprE was significantly reduced. The difference in the protease production capacity of Bacillus was found to be related to the expression level of aprX by further studying the mRNA level of the gene.【Conclusion】There is a correlation between the protease gene carried on the chromosome of the strain and the protease production capacity of the strain. The important enzyme gene that affects the protease production capacity of Bacillus is aprX.http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202205013proteasebacilluscomparative genomicsfluorescence quantitative pcraprx gene |
| spellingShingle | Zhongxin JIA Jianan ZHAO Fang JI Xue WANG Gang LI Chengmin WANG Jianhua QIN Comparative Genomics Analysis of Protease Genes in Bacillus sp. Guangdong nongye kexue protease bacillus comparative genomics fluorescence quantitative pcr aprx gene |
| title | Comparative Genomics Analysis of Protease Genes in Bacillus sp. |
| title_full | Comparative Genomics Analysis of Protease Genes in Bacillus sp. |
| title_fullStr | Comparative Genomics Analysis of Protease Genes in Bacillus sp. |
| title_full_unstemmed | Comparative Genomics Analysis of Protease Genes in Bacillus sp. |
| title_short | Comparative Genomics Analysis of Protease Genes in Bacillus sp. |
| title_sort | comparative genomics analysis of protease genes in bacillus sp |
| topic | protease bacillus comparative genomics fluorescence quantitative pcr aprx gene |
| url | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202205013 |
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