Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential
Anaerobic digestion for the valorization of organic wastes into biogas is gaining worldwide interest. Nonetheless, the sizing of the biogas plant units require knowledge of the quantity of feedstock, and their associated methane potentials, estimated widely by Biochemical Methane Potential (BMP) tes...
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MDPI AG
2021-11-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/8/11/176 |
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| author | Audrey Lallement Aline Siaud Christine Peyrelasse Prasad Kaparaju Blandine Schraauwers Samuel Maunas Florian Monlau |
| author_facet | Audrey Lallement Aline Siaud Christine Peyrelasse Prasad Kaparaju Blandine Schraauwers Samuel Maunas Florian Monlau |
| author_sort | Audrey Lallement |
| collection | DOAJ |
| description | Anaerobic digestion for the valorization of organic wastes into biogas is gaining worldwide interest. Nonetheless, the sizing of the biogas plant units require knowledge of the quantity of feedstock, and their associated methane potentials, estimated widely by Biochemical Methane Potential (BMP) tests. Discrepancies exist among laboratories due to variability of protocols adopted and operational factors used. The aim of this study is to verify the influence of some operational factors (e.g., analysis frequency, trace elements and vitamins solution addition and flushing gas), feedstock conservation and the source of inoculum on BMP. Among the operational parameters tested on cellulose degradation, only the type of gas used for flushing headspace of BMP assays had shown a significant influence on methane yields from cellulose. Methane yields of 344 ± 6 NL CH<sub>4</sub> kg<sup>−1</sup> VS and 321 ± 10 NL CH<sub>4</sub> kg<sup>−1</sup> VS obtained from assays flushed with pure N<sub>2</sub> and N<sub>2</sub>/CO<sub>2</sub> (60/40 <i>v</i>/<i>v</i>). The origin of inoculum (fed in co-digestion) only significantly affected the methane yields for straw, 253 ± 3 and 333 ± 3 NL CH<sub>4</sub> kg<sup>−1</sup> VS. Finally, freezing/thawing cycle effect depended of the substrate (tested on biowaste, manure, straw and WWTP sludge) with a possible effect of water content substrate. |
| first_indexed | 2024-03-10T05:42:09Z |
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| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T05:42:09Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
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| series | Bioengineering |
| spelling | doaj.art-5d3357b02f8a487980facabde85ef4962023-11-22T22:26:42ZengMDPI AGBioengineering2306-53542021-11-0181117610.3390/bioengineering8110176Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane PotentialAudrey Lallement0Aline Siaud1Christine Peyrelasse2Prasad Kaparaju3Blandine Schraauwers4Samuel Maunas5Florian Monlau6APESA, Pôle Valorisation, Cap Ecologia, Avenue Fréderic Joliot Curie, 64230 Lescar, FranceAPESA, Pôle Valorisation, Cap Ecologia, Avenue Fréderic Joliot Curie, 64230 Lescar, FranceAPESA, Pôle Valorisation, Cap Ecologia, Avenue Fréderic Joliot Curie, 64230 Lescar, FranceSchool of Engineering and Built Environment, Nathan Campus, Griffith University, Brisbane, QLD 4111, AustraliaAPESA, Pôle Valorisation, Cap Ecologia, Avenue Fréderic Joliot Curie, 64230 Lescar, FranceAPESA, Pôle Valorisation, Cap Ecologia, Avenue Fréderic Joliot Curie, 64230 Lescar, FranceAPESA, Pôle Valorisation, Cap Ecologia, Avenue Fréderic Joliot Curie, 64230 Lescar, FranceAnaerobic digestion for the valorization of organic wastes into biogas is gaining worldwide interest. Nonetheless, the sizing of the biogas plant units require knowledge of the quantity of feedstock, and their associated methane potentials, estimated widely by Biochemical Methane Potential (BMP) tests. Discrepancies exist among laboratories due to variability of protocols adopted and operational factors used. The aim of this study is to verify the influence of some operational factors (e.g., analysis frequency, trace elements and vitamins solution addition and flushing gas), feedstock conservation and the source of inoculum on BMP. Among the operational parameters tested on cellulose degradation, only the type of gas used for flushing headspace of BMP assays had shown a significant influence on methane yields from cellulose. Methane yields of 344 ± 6 NL CH<sub>4</sub> kg<sup>−1</sup> VS and 321 ± 10 NL CH<sub>4</sub> kg<sup>−1</sup> VS obtained from assays flushed with pure N<sub>2</sub> and N<sub>2</sub>/CO<sub>2</sub> (60/40 <i>v</i>/<i>v</i>). The origin of inoculum (fed in co-digestion) only significantly affected the methane yields for straw, 253 ± 3 and 333 ± 3 NL CH<sub>4</sub> kg<sup>−1</sup> VS. Finally, freezing/thawing cycle effect depended of the substrate (tested on biowaste, manure, straw and WWTP sludge) with a possible effect of water content substrate.https://www.mdpi.com/2306-5354/8/11/176anaerobic digestioninoculum originfeedstock conservationkinetic rate |
| spellingShingle | Audrey Lallement Aline Siaud Christine Peyrelasse Prasad Kaparaju Blandine Schraauwers Samuel Maunas Florian Monlau Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential Bioengineering anaerobic digestion inoculum origin feedstock conservation kinetic rate |
| title | Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential |
| title_full | Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential |
| title_fullStr | Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential |
| title_full_unstemmed | Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential |
| title_short | Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential |
| title_sort | impact of operational factors inoculum origin and feedstock preservation on the biochemical methane potential |
| topic | anaerobic digestion inoculum origin feedstock conservation kinetic rate |
| url | https://www.mdpi.com/2306-5354/8/11/176 |
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