Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound Infection
Background: Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen with high antibiotic resistance and biofilm production capability in the patients with burn wound. The present study aimed to determine the antibiotic resistance pattern and frequency of the multidrug-resistant (MDR) str...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2020-01-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/12561 |
| _version_ | 1797728334996570112 |
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| author | Elham Haghighifar Bahram Nasr-Esfahani Hossein Fazeli |
| author_facet | Elham Haghighifar Bahram Nasr-Esfahani Hossein Fazeli |
| author_sort | Elham Haghighifar |
| collection | DOAJ |
| description | Background: Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen with high antibiotic resistance and biofilm production capability in the patients with burn wound. The present study aimed to determine the antibiotic resistance pattern and frequency of the multidrug-resistant (MDR) strains, and evaluate biofilm formation potential in the burn wound infection isolates.
Methods: Biochemical and molecular tests were used for identification of the 117 strains collected from Isfahan City, Iran, during one-year period. Antibacterial susceptibility test was performed using disk diffusion and interpreted in accordance with the standards of Clinical and Laboratory Standards Institute (CLSI). Biofilm formation was evaluated using microtiter plate assay.
Findings: Of 117 clinical strains of A. baumannii, 114 (97.49%) were MDR. Strains showed maximum resistant to ceftazidime (99.14%) followed by ciprofloxacin (94.01%). 81.19% of isolates were able to form biofilm, 1.7% of strains were strong-biofilm producers, and 18.80% of them were notable biofilm producers. In this assessment, 42.73% and 36.75% were considered as moderate and weak biofilm-forming isolates, respectively.
Conclusion: The high prevalence of A. baumannii in patients with burn wound, and the significant relationship between biofilm formation and resistance among the burn isolates indicate that antibiotic resistance is increasing rapidly, and soon many usable antibiotics will become ineffective medicines for treating patients. This needs an emergency actions and alternative ways to control these problems. |
| first_indexed | 2024-03-12T11:12:34Z |
| format | Article |
| id | doaj.art-5d34a02f6b0141bcbeb248a9dfcb8c34 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T11:12:34Z |
| publishDate | 2020-01-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-5d34a02f6b0141bcbeb248a9dfcb8c342023-09-02T02:33:32ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2020-01-01375521263127110.22122/jims.v37i552.125613523Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound InfectionElham Haghighifar0Bahram Nasr-Esfahani1Hossein Fazeli2MSc Student, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranProfessor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen with high antibiotic resistance and biofilm production capability in the patients with burn wound. The present study aimed to determine the antibiotic resistance pattern and frequency of the multidrug-resistant (MDR) strains, and evaluate biofilm formation potential in the burn wound infection isolates. Methods: Biochemical and molecular tests were used for identification of the 117 strains collected from Isfahan City, Iran, during one-year period. Antibacterial susceptibility test was performed using disk diffusion and interpreted in accordance with the standards of Clinical and Laboratory Standards Institute (CLSI). Biofilm formation was evaluated using microtiter plate assay. Findings: Of 117 clinical strains of A. baumannii, 114 (97.49%) were MDR. Strains showed maximum resistant to ceftazidime (99.14%) followed by ciprofloxacin (94.01%). 81.19% of isolates were able to form biofilm, 1.7% of strains were strong-biofilm producers, and 18.80% of them were notable biofilm producers. In this assessment, 42.73% and 36.75% were considered as moderate and weak biofilm-forming isolates, respectively. Conclusion: The high prevalence of A. baumannii in patients with burn wound, and the significant relationship between biofilm formation and resistance among the burn isolates indicate that antibiotic resistance is increasing rapidly, and soon many usable antibiotics will become ineffective medicines for treating patients. This needs an emergency actions and alternative ways to control these problems.http://jims.mui.ac.ir/index.php/jims/article/view/12561antibiotic resistancebiofilmacinetobacter baumanniiburns, woundsinfection |
| spellingShingle | Elham Haghighifar Bahram Nasr-Esfahani Hossein Fazeli Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound Infection مجله دانشکده پزشکی اصفهان antibiotic resistance biofilm acinetobacter baumannii burns , wounds infection |
| title | Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound Infection |
| title_full | Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound Infection |
| title_fullStr | Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound Infection |
| title_full_unstemmed | Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound Infection |
| title_short | Determination of Biofilm Formation Ability and Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Patients with Burn Wound Infection |
| title_sort | determination of biofilm formation ability and antibiotic resistance of acinetobacter baumannii strains isolated from patients with burn wound infection |
| topic | antibiotic resistance biofilm acinetobacter baumannii burns , wounds infection |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/12561 |
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