Symbiodinium spp. associated with scleractinian corals from Dongsha Atoll (Pratas), Taiwan, in the South China Sea
Dongsha Atoll (also known as Pratas) in Taiwan is the northernmost atoll in the South China Sea and a designated marine national park since 2007. The marine park’s scope of protection covers the bio-resources of its waters in addition to uplands, so it is important to have data logging information a...
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| Format: | Article |
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PeerJ Inc.
2017-01-01
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| Online Access: | https://peerj.com/articles/2871.pdf |
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| author | Shashank Keshavmurthy Kuo-Hsun Tang Chia-Min Hsu Chai-Hsia Gan Chao-Yang Kuo Keryea Soong Hong-Nong Chou Chaolun Allen Chen |
| author_facet | Shashank Keshavmurthy Kuo-Hsun Tang Chia-Min Hsu Chai-Hsia Gan Chao-Yang Kuo Keryea Soong Hong-Nong Chou Chaolun Allen Chen |
| author_sort | Shashank Keshavmurthy |
| collection | DOAJ |
| description | Dongsha Atoll (also known as Pratas) in Taiwan is the northernmost atoll in the South China Sea and a designated marine national park since 2007. The marine park’s scope of protection covers the bio-resources of its waters in addition to uplands, so it is important to have data logging information and analyses of marine flora and fauna, including their physiology, ecology, and genetics. As part of this effort, we investigated Symbiodinium associations in scleractinian corals from Dongsha Atoll through surveys carried out at two depth ranges (shallow, 1–5 m; and deep, 10–15 m) in 2009 and during a bleaching event in 2010. Symbiodinium composition was assessed using restriction fragment length polymorphism (RFLP) of 28S nuclear large subunit ribosomal DNA (nlsrDNA). Our results showed that the 796 coral samples from seven families and 20 genera collected in 2009 and 132 coral samples from seven families and 12 genera collected in 2010 were associated with Symbiodinium C, D and C+D. Occurrence of clade D in shallow water (24.5%) was higher compared to deep (14.9%). Due to a bleaching event in 2010, up to 80% of coral species associated with Symbiodinium C underwent moderate to severe bleaching. Using the fine resolution technique of denaturing gradient gel electrophoresis (DGGE) of internal transcribed spacer 2 (ITS2) in 175 randomly selected coral samples, from 2009 and 2010, eight Symbiodinium C types and two Symbiodinium D types were detected. This study is the first baseline survey on Symbiodinium associations in the corals of Dongsha Atoll in the South China Sea, and it shows the dominance of Symbiodinium clade C in the population. |
| first_indexed | 2024-03-09T07:27:08Z |
| format | Article |
| id | doaj.art-5d3879e349e3457495350b0503d08cde |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:27:08Z |
| publishDate | 2017-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-5d3879e349e3457495350b0503d08cde2023-12-03T06:52:18ZengPeerJ Inc.PeerJ2167-83592017-01-015e287110.7717/peerj.2871Symbiodinium spp. associated with scleractinian corals from Dongsha Atoll (Pratas), Taiwan, in the South China SeaShashank Keshavmurthy0Kuo-Hsun Tang1Chia-Min Hsu2Chai-Hsia Gan3Chao-Yang Kuo4Keryea Soong5Hong-Nong Chou6Chaolun Allen Chen7Biodiversity Research Center, Academia Sinica, Taipei, TaiwanBiodiversity Research Center, Academia Sinica, Taipei, TaiwanBiodiversity Research Center, Academia Sinica, Taipei, TaiwanBiodiversity Research Center, Academia Sinica, Taipei, TaiwanBiodiversity Research Center, Academia Sinica, Taipei, TaiwanDepartment of Oceanography, Dongsha Atoll Research Station, National Sun Yat-Sen University, Kaohsuing, TaiwanInstitute of Fisheries Science, National Taiwan University, Taipei, TaiwanBiodiversity Research Center, Academia Sinica, Taipei, TaiwanDongsha Atoll (also known as Pratas) in Taiwan is the northernmost atoll in the South China Sea and a designated marine national park since 2007. The marine park’s scope of protection covers the bio-resources of its waters in addition to uplands, so it is important to have data logging information and analyses of marine flora and fauna, including their physiology, ecology, and genetics. As part of this effort, we investigated Symbiodinium associations in scleractinian corals from Dongsha Atoll through surveys carried out at two depth ranges (shallow, 1–5 m; and deep, 10–15 m) in 2009 and during a bleaching event in 2010. Symbiodinium composition was assessed using restriction fragment length polymorphism (RFLP) of 28S nuclear large subunit ribosomal DNA (nlsrDNA). Our results showed that the 796 coral samples from seven families and 20 genera collected in 2009 and 132 coral samples from seven families and 12 genera collected in 2010 were associated with Symbiodinium C, D and C+D. Occurrence of clade D in shallow water (24.5%) was higher compared to deep (14.9%). Due to a bleaching event in 2010, up to 80% of coral species associated with Symbiodinium C underwent moderate to severe bleaching. Using the fine resolution technique of denaturing gradient gel electrophoresis (DGGE) of internal transcribed spacer 2 (ITS2) in 175 randomly selected coral samples, from 2009 and 2010, eight Symbiodinium C types and two Symbiodinium D types were detected. This study is the first baseline survey on Symbiodinium associations in the corals of Dongsha Atoll in the South China Sea, and it shows the dominance of Symbiodinium clade C in the population.https://peerj.com/articles/2871.pdfSymbiodinium associationDongsha AtollSymbiodinium CSymbiodinium glyniiSouth China seaSymbiodinium trenchii |
| spellingShingle | Shashank Keshavmurthy Kuo-Hsun Tang Chia-Min Hsu Chai-Hsia Gan Chao-Yang Kuo Keryea Soong Hong-Nong Chou Chaolun Allen Chen Symbiodinium spp. associated with scleractinian corals from Dongsha Atoll (Pratas), Taiwan, in the South China Sea PeerJ Symbiodinium association Dongsha Atoll Symbiodinium C Symbiodinium glynii South China sea Symbiodinium trenchii |
| title | Symbiodinium spp. associated with scleractinian corals from Dongsha Atoll (Pratas), Taiwan, in the South China Sea |
| title_full | Symbiodinium spp. associated with scleractinian corals from Dongsha Atoll (Pratas), Taiwan, in the South China Sea |
| title_fullStr | Symbiodinium spp. associated with scleractinian corals from Dongsha Atoll (Pratas), Taiwan, in the South China Sea |
| title_full_unstemmed | Symbiodinium spp. associated with scleractinian corals from Dongsha Atoll (Pratas), Taiwan, in the South China Sea |
| title_short | Symbiodinium spp. associated with scleractinian corals from Dongsha Atoll (Pratas), Taiwan, in the South China Sea |
| title_sort | symbiodinium spp associated with scleractinian corals from dongsha atoll pratas taiwan in the south china sea |
| topic | Symbiodinium association Dongsha Atoll Symbiodinium C Symbiodinium glynii South China sea Symbiodinium trenchii |
| url | https://peerj.com/articles/2871.pdf |
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