Influence of Repetitive Transcranial Magnetic Stimulation on Human Neurochemistry and Functional Connectivity: A Pilot MRI/MRS Study at 7 T
Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive brain stimulation method commonly used in the disciplines of neuroscience, neurology, and neuropsychiatry to examine or modulate brain function. Low frequency rTMS (e.g., 1 Hz) is associated with a net suppression of cortical exci...
| Main Authors: | , , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Acceso en liña: | https://www.frontiersin.org/article/10.3389/fnins.2019.01260/full |
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| author | Heidi Gröhn Heidi Gröhn Bernadette T. Gillick Ivan Tkáč Petr Bednařík Petr Bednařík Daniele Mascali Dinesh K. Deelchand Shalom Michaeli Gregg D. Meekins Michael J. Leffler-McCabe Colum D. MacKinnon Lynn E. Eberly Silvia Mangia |
| author_facet | Heidi Gröhn Heidi Gröhn Bernadette T. Gillick Ivan Tkáč Petr Bednařík Petr Bednařík Daniele Mascali Dinesh K. Deelchand Shalom Michaeli Gregg D. Meekins Michael J. Leffler-McCabe Colum D. MacKinnon Lynn E. Eberly Silvia Mangia |
| author_sort | Heidi Gröhn |
| collection | DOAJ |
| description | Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive brain stimulation method commonly used in the disciplines of neuroscience, neurology, and neuropsychiatry to examine or modulate brain function. Low frequency rTMS (e.g., 1 Hz) is associated with a net suppression of cortical excitability, whereas higher frequencies (e.g., 5 Hz) purportedly increase excitability. Magnetic resonance spectroscopy (MRS) and resting-state functional MRI (rsfMRI) allow investigation of neurochemistry and functional connectivity, respectively, and can assess the influence of rTMS in these domains. This pilot study investigated the effects of rTMS on the primary motor cortex using pre and post MRS and rsfMRI assessments at 7 T. Seven right-handed males (age 27 ± 7 y.o.) underwent single-voxel MRS and rsfMRI before and about 30-min after rTMS was administered outside the scanner for 20-min over the primary motor cortex of the left (dominant) hemisphere. All participants received 1-Hz rTMS; one participant additionally received 5-Hz rTMS in a separate session. Concentrations of 17 neurochemicals were quantified in left and right motor cortices. Connectivity metrics included fractional amplitude of low-frequency fluctuations (fALFF) and regional homogeneity (ReHo) of both motor cortices, strength of related brain networks, and inter-hemispheric connectivity. The group-analysis revealed few trends (i.e., uncorrected for multiple comparisons), including a mean increase in the concentration of the inhibitory neurotransmitter γ-aminobutyric acid (GABA) after the inhibitory rTMS protocol as compared to baseline in the stimulated (left) motor cortex (+8%, p = 0.043), along with a slight increase of total creatine (+2%, p = 0.018), and decrease of aspartate (−18%, p = 0.016). Additionally, GABA tended to decrease in the contralateral hemisphere (−6%, p = 0.033). No other changes of metabolite concentrations were found. Whereas functional connectivity outcomes did not exhibit trends of significant changes induced by rTMS, the percent changes of few connectivity metrics in both hemispheres were negatively correlated with GABA changes in the contralateral hemisphere. While studies in larger cohorts are needed to confirm these preliminary findings, our results indicate the safety and feasibility of detecting changes in key metabolites associated with neurotransmission after a single 1-Hz rTMS session, establishing the construct for future exploration of the neurochemical, and connectivity mechanisms of cortical responses to neuromodulation. |
| first_indexed | 2024-04-12T05:45:21Z |
| format | Article |
| id | doaj.art-5d3bb3f49a6d4bd39c7eebccf8c873f4 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-12T05:45:21Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-5d3bb3f49a6d4bd39c7eebccf8c873f42022-12-22T03:45:28ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2019-11-011310.3389/fnins.2019.01260476844Influence of Repetitive Transcranial Magnetic Stimulation on Human Neurochemistry and Functional Connectivity: A Pilot MRI/MRS Study at 7 THeidi Gröhn0Heidi Gröhn1Bernadette T. Gillick2Ivan Tkáč3Petr Bednařík4Petr Bednařík5Daniele Mascali6Dinesh K. Deelchand7Shalom Michaeli8Gregg D. Meekins9Michael J. Leffler-McCabe10Colum D. MacKinnon11Lynn E. Eberly12Silvia Mangia13Department of Radiology, Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United StatesDiagnostic Imaging Center, Kuopio University Hospital, Kuopio, FinlandDepartment of Rehabilitation Medicine, University of Minnesota, Minneapolis, MN, United StatesDepartment of Radiology, Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United StatesDepartment of Radiology, Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United StatesDepartment of Biomedical Imaging and Image-guided Therapy, High Field MR Centre, Medical University of Vienna, Vienna, AustriaMuseo Storico della Fisica e Centro Studi e Ricerche “Enrico Fermi”, Rome, ItalyDepartment of Radiology, Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United StatesDepartment of Radiology, Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United StatesDepartment of Neurology, University of Minnesota, Minneapolis, MN, United StatesDepartment of Neurology, University of Minnesota, Minneapolis, MN, United StatesDepartment of Neurology, University of Minnesota, Minneapolis, MN, United StatesDivision of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN, United StatesDepartment of Radiology, Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United StatesRepetitive transcranial magnetic stimulation (rTMS) is a non-invasive brain stimulation method commonly used in the disciplines of neuroscience, neurology, and neuropsychiatry to examine or modulate brain function. Low frequency rTMS (e.g., 1 Hz) is associated with a net suppression of cortical excitability, whereas higher frequencies (e.g., 5 Hz) purportedly increase excitability. Magnetic resonance spectroscopy (MRS) and resting-state functional MRI (rsfMRI) allow investigation of neurochemistry and functional connectivity, respectively, and can assess the influence of rTMS in these domains. This pilot study investigated the effects of rTMS on the primary motor cortex using pre and post MRS and rsfMRI assessments at 7 T. Seven right-handed males (age 27 ± 7 y.o.) underwent single-voxel MRS and rsfMRI before and about 30-min after rTMS was administered outside the scanner for 20-min over the primary motor cortex of the left (dominant) hemisphere. All participants received 1-Hz rTMS; one participant additionally received 5-Hz rTMS in a separate session. Concentrations of 17 neurochemicals were quantified in left and right motor cortices. Connectivity metrics included fractional amplitude of low-frequency fluctuations (fALFF) and regional homogeneity (ReHo) of both motor cortices, strength of related brain networks, and inter-hemispheric connectivity. The group-analysis revealed few trends (i.e., uncorrected for multiple comparisons), including a mean increase in the concentration of the inhibitory neurotransmitter γ-aminobutyric acid (GABA) after the inhibitory rTMS protocol as compared to baseline in the stimulated (left) motor cortex (+8%, p = 0.043), along with a slight increase of total creatine (+2%, p = 0.018), and decrease of aspartate (−18%, p = 0.016). Additionally, GABA tended to decrease in the contralateral hemisphere (−6%, p = 0.033). No other changes of metabolite concentrations were found. Whereas functional connectivity outcomes did not exhibit trends of significant changes induced by rTMS, the percent changes of few connectivity metrics in both hemispheres were negatively correlated with GABA changes in the contralateral hemisphere. While studies in larger cohorts are needed to confirm these preliminary findings, our results indicate the safety and feasibility of detecting changes in key metabolites associated with neurotransmission after a single 1-Hz rTMS session, establishing the construct for future exploration of the neurochemical, and connectivity mechanisms of cortical responses to neuromodulation.https://www.frontiersin.org/article/10.3389/fnins.2019.01260/fullGABArepetitive transcranial magnetic stimulationmagnetic resonance spectroscopyresting-state functional MRImotor cortexinhibition |
| spellingShingle | Heidi Gröhn Heidi Gröhn Bernadette T. Gillick Ivan Tkáč Petr Bednařík Petr Bednařík Daniele Mascali Dinesh K. Deelchand Shalom Michaeli Gregg D. Meekins Michael J. Leffler-McCabe Colum D. MacKinnon Lynn E. Eberly Silvia Mangia Influence of Repetitive Transcranial Magnetic Stimulation on Human Neurochemistry and Functional Connectivity: A Pilot MRI/MRS Study at 7 T Frontiers in Neuroscience GABA repetitive transcranial magnetic stimulation magnetic resonance spectroscopy resting-state functional MRI motor cortex inhibition |
| title | Influence of Repetitive Transcranial Magnetic Stimulation on Human Neurochemistry and Functional Connectivity: A Pilot MRI/MRS Study at 7 T |
| title_full | Influence of Repetitive Transcranial Magnetic Stimulation on Human Neurochemistry and Functional Connectivity: A Pilot MRI/MRS Study at 7 T |
| title_fullStr | Influence of Repetitive Transcranial Magnetic Stimulation on Human Neurochemistry and Functional Connectivity: A Pilot MRI/MRS Study at 7 T |
| title_full_unstemmed | Influence of Repetitive Transcranial Magnetic Stimulation on Human Neurochemistry and Functional Connectivity: A Pilot MRI/MRS Study at 7 T |
| title_short | Influence of Repetitive Transcranial Magnetic Stimulation on Human Neurochemistry and Functional Connectivity: A Pilot MRI/MRS Study at 7 T |
| title_sort | influence of repetitive transcranial magnetic stimulation on human neurochemistry and functional connectivity a pilot mri mrs study at 7 t |
| topic | GABA repetitive transcranial magnetic stimulation magnetic resonance spectroscopy resting-state functional MRI motor cortex inhibition |
| url | https://www.frontiersin.org/article/10.3389/fnins.2019.01260/full |
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