Effects of co-exposure to CS2 and noise on hearing and balance in rats: continuous versus intermittent CS2 exposures
Abstract Background Carbon disulfide (CS2) exacerbates the effect of noise on hearing, and disrupts the vestibular system. The goal of this study was to determine whether these effects are also observed with intermittent CS2 exposure. Methods Rats were exposed for 4 weeks (5 days/week, 6 h/day) to a...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | Journal of Occupational Medicine and Toxicology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12995-020-00260-5 |
| _version_ | 1798041851754708992 |
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| author | Monique Chalansonnet Maria Carreres-Pons Thomas Venet Aurélie Thomas Lise Merlen Stéphane Boucard Frédéric Cosnier Hervé Nunge Elodie Bonfanti Jordi Llorens Pierre Campo Benoît Pouyatos |
| author_facet | Monique Chalansonnet Maria Carreres-Pons Thomas Venet Aurélie Thomas Lise Merlen Stéphane Boucard Frédéric Cosnier Hervé Nunge Elodie Bonfanti Jordi Llorens Pierre Campo Benoît Pouyatos |
| author_sort | Monique Chalansonnet |
| collection | DOAJ |
| description | Abstract Background Carbon disulfide (CS2) exacerbates the effect of noise on hearing, and disrupts the vestibular system. The goal of this study was to determine whether these effects are also observed with intermittent CS2 exposure. Methods Rats were exposed for 4 weeks (5 days/week, 6 h/day) to a band noise at 106 dB SPL either alone or combined with continuous (63 ppm or 250 ppm) or intermittent (15 min/h or 2 × 15 min/h at 250 ppm) CS2. Hearing function was assessed by measuring distortion product otoacoustic emissions (DPOAEs); balance was monitored based on the vestibulo-ocular reflex (VOR). Functional measurements were performed before, at the end of exposure and 4 weeks later. Histological analyses of the inner ear were also performed following exposure and after the 4-week recovery period. Results The results obtained here confirmed that CS2 exposure exerts two differential temporary effects on hearing: (1) it attenuates the noise-induced DPOAE decrease below 6 kHz probably through action on the middle ear reflex when exposure lasts 15 min per hour, and (2) continuous exposure to 250 ppm for 6 h extends the frequency range affected by noise up to 9.6 kHz (instead of 6 kHz with noise alone). With regard to balance, the VOR was reversibly disrupted at the two highest doses of CS2 (2 × 15 min/h and continuous 250 ppm). No morphological alterations to the inner ear were observed. Conclusion These results reveal that short periods of CS2 exposure can alter the sensitivity of the cochlea to noise at a dose equivalent to only 10 times the short-term occupational limit value, and intermittent exposure to CS2 (2 × 15 min/h) can alter the function of the vestibular system. |
| first_indexed | 2024-04-11T22:27:21Z |
| format | Article |
| id | doaj.art-5d3ce5f47d1c4b9c9d8eb6b9aa8cac48 |
| institution | Directory Open Access Journal |
| issn | 1745-6673 |
| language | English |
| last_indexed | 2024-04-11T22:27:21Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Occupational Medicine and Toxicology |
| spelling | doaj.art-5d3ce5f47d1c4b9c9d8eb6b9aa8cac482022-12-22T03:59:37ZengBMCJournal of Occupational Medicine and Toxicology1745-66732020-05-0115111110.1186/s12995-020-00260-5Effects of co-exposure to CS2 and noise on hearing and balance in rats: continuous versus intermittent CS2 exposuresMonique Chalansonnet0Maria Carreres-Pons1Thomas Venet2Aurélie Thomas3Lise Merlen4Stéphane Boucard5Frédéric Cosnier6Hervé Nunge7Elodie Bonfanti8Jordi Llorens9Pierre Campo10Benoît Pouyatos11Institut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanDepartament de Ciències Fisiològiques and Institute of Neurosciences, Universitat de BarcelonaInstitut National de Recherche et de Sécurité, Rue du MorvanInstitut National de Recherche et de Sécurité, Rue du MorvanAbstract Background Carbon disulfide (CS2) exacerbates the effect of noise on hearing, and disrupts the vestibular system. The goal of this study was to determine whether these effects are also observed with intermittent CS2 exposure. Methods Rats were exposed for 4 weeks (5 days/week, 6 h/day) to a band noise at 106 dB SPL either alone or combined with continuous (63 ppm or 250 ppm) or intermittent (15 min/h or 2 × 15 min/h at 250 ppm) CS2. Hearing function was assessed by measuring distortion product otoacoustic emissions (DPOAEs); balance was monitored based on the vestibulo-ocular reflex (VOR). Functional measurements were performed before, at the end of exposure and 4 weeks later. Histological analyses of the inner ear were also performed following exposure and after the 4-week recovery period. Results The results obtained here confirmed that CS2 exposure exerts two differential temporary effects on hearing: (1) it attenuates the noise-induced DPOAE decrease below 6 kHz probably through action on the middle ear reflex when exposure lasts 15 min per hour, and (2) continuous exposure to 250 ppm for 6 h extends the frequency range affected by noise up to 9.6 kHz (instead of 6 kHz with noise alone). With regard to balance, the VOR was reversibly disrupted at the two highest doses of CS2 (2 × 15 min/h and continuous 250 ppm). No morphological alterations to the inner ear were observed. Conclusion These results reveal that short periods of CS2 exposure can alter the sensitivity of the cochlea to noise at a dose equivalent to only 10 times the short-term occupational limit value, and intermittent exposure to CS2 (2 × 15 min/h) can alter the function of the vestibular system.http://link.springer.com/article/10.1186/s12995-020-00260-5Carbon disulfideLow-frequency noiseCochleaVestibuleCo-exposureRat |
| spellingShingle | Monique Chalansonnet Maria Carreres-Pons Thomas Venet Aurélie Thomas Lise Merlen Stéphane Boucard Frédéric Cosnier Hervé Nunge Elodie Bonfanti Jordi Llorens Pierre Campo Benoît Pouyatos Effects of co-exposure to CS2 and noise on hearing and balance in rats: continuous versus intermittent CS2 exposures Journal of Occupational Medicine and Toxicology Carbon disulfide Low-frequency noise Cochlea Vestibule Co-exposure Rat |
| title | Effects of co-exposure to CS2 and noise on hearing and balance in rats: continuous versus intermittent CS2 exposures |
| title_full | Effects of co-exposure to CS2 and noise on hearing and balance in rats: continuous versus intermittent CS2 exposures |
| title_fullStr | Effects of co-exposure to CS2 and noise on hearing and balance in rats: continuous versus intermittent CS2 exposures |
| title_full_unstemmed | Effects of co-exposure to CS2 and noise on hearing and balance in rats: continuous versus intermittent CS2 exposures |
| title_short | Effects of co-exposure to CS2 and noise on hearing and balance in rats: continuous versus intermittent CS2 exposures |
| title_sort | effects of co exposure to cs2 and noise on hearing and balance in rats continuous versus intermittent cs2 exposures |
| topic | Carbon disulfide Low-frequency noise Cochlea Vestibule Co-exposure Rat |
| url | http://link.springer.com/article/10.1186/s12995-020-00260-5 |
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