A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain
Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that Bordetella pertussis...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-03-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/2267 |
| _version_ | 1818881225537880064 |
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| author | Mohammad Sekhavati Ashraf Mohabati Mobarez Seyed Davar Siadat Mojtaba Noofeli |
| author_facet | Mohammad Sekhavati Ashraf Mohabati Mobarez Seyed Davar Siadat Mojtaba Noofeli |
| author_sort | Mohammad Sekhavati |
| collection | DOAJ |
| description | Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis.
Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs.
Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay.
Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis. |
| first_indexed | 2024-12-19T14:58:29Z |
| format | Article |
| id | doaj.art-5d3df33254c845778371148287fe5704 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-19T14:58:29Z |
| publishDate | 2020-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-5d3df33254c845778371148287fe57042022-12-21T20:16:38ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472020-03-0112110.18502/ijm.v12i1.2516A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strainMohammad Sekhavati0Ashraf Mohabati Mobarez1Seyed Davar Siadat2Mojtaba Noofeli3Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranMicrobiology Research Center (MRC) Pasteur, Institute of Iran, Tehran, IranDepartment of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranBackground and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis. Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs. Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay. Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.https://ijm.tums.ac.ir/index.php/ijm/article/view/2267Bordetella pertussisExtractionOuter membrane vesicleVaccine |
| spellingShingle | Mohammad Sekhavati Ashraf Mohabati Mobarez Seyed Davar Siadat Mojtaba Noofeli A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain Iranian Journal of Microbiology Bordetella pertussis Extraction Outer membrane vesicle Vaccine |
| title | A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain |
| title_full | A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain |
| title_fullStr | A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain |
| title_full_unstemmed | A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain |
| title_short | A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain |
| title_sort | novel method for the extraction of outer membrane vesicles omvs from bordetella pertussis tohama strain |
| topic | Bordetella pertussis Extraction Outer membrane vesicle Vaccine |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/2267 |
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