Improved HaloTag Ligand Enables BRET Imaging With NanoLuc

Improved HaloTag Ligand Enables BRET Imaging With NanoLuc

Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions. The recommended use of HaloTag618 ligand for the NanoLuc-HaloTag BRET pair is versatile for ensemble experiments d...

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Bibliographic Details
Main Authors: Ovia Margaret Thirukkumaran, Congrong Wang, Nnamdi Joseph Asouzu, Eduard Fron, Susana Rocha, Johan Hofkens, Luke D. Lavis, Hideaki Mizuno
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-01-01
Series:Frontiers in Chemistry
Subjects:
NanoLuc
HaloTag
BRET imaging
Janelia Fluor dyes
PKA
Online Access:https://www.frontiersin.org/article/10.3389/fchem.2019.00938/full

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