CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model
Rotator cuff tendon (RCT) disease results from multifactorial mechanisms, in which inflammation plays a key role. Pro-inflammatory cytokines and tendon stem cell/progenitor cells (TSPCs) have been shown to participate in the inflammatory response. However, the underlying molecular mechanism is still...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/15165 |
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| author | Raffaella Giancola Francesco Oliva Marialucia Gallorini Noemi Michetti Clarissa Gissi Fadl Moussa Cristina Antonetti Lamorgese Passeri Alessia Colosimo Anna Concetta Berardi |
| author_facet | Raffaella Giancola Francesco Oliva Marialucia Gallorini Noemi Michetti Clarissa Gissi Fadl Moussa Cristina Antonetti Lamorgese Passeri Alessia Colosimo Anna Concetta Berardi |
| author_sort | Raffaella Giancola |
| collection | DOAJ |
| description | Rotator cuff tendon (RCT) disease results from multifactorial mechanisms, in which inflammation plays a key role. Pro-inflammatory cytokines and tendon stem cell/progenitor cells (TSPCs) have been shown to participate in the inflammatory response. However, the underlying molecular mechanism is still not clear. In this study, flow cytometry analyses of different subpopulations of RCT-derived TSPCs demonstrate that after three days of administration, TNFα alone or in combination with IFNγ significantly decreases the percentage of CD146+CD49d+ and CD146+CD49f+ but not CD146+CD109+ TSPCs populations. In parallel, the same pro-inflammatory cytokines upregulate the expression of CD200 in the CD146+ TSPCs population. Additionally, the TNFα/IFNγ combination modulates the protein expression of STAT1, STAT3, and MMP9, but not fibromodulin. At the gene level, <i>IRF1</i>, <i>CAAT (CAAT/EBPbeta)</i>, and <i>DOK2</i> but not <i>NF-κb</i>, <i>TGRF2 (TGFBR2)</i>, and <i>RAS-GAP</i> are modulated. In conclusion, although our study has several important limitations, the results highlight a new potential role of CD200 in regulating inflammation during tendon injuries. In addition, the genes analyzed here might be new potential players in the inflammatory response of TSPCs. |
| first_indexed | 2024-03-09T17:44:29Z |
| format | Article |
| id | doaj.art-5d3f91e5fa434d0f9e6311266659cc36 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:44:29Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5d3f91e5fa434d0f9e6311266659cc362023-11-24T11:14:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123231516510.3390/ijms232315165CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro ModelRaffaella Giancola0Francesco Oliva1Marialucia Gallorini2Noemi Michetti3Clarissa Gissi4Fadl Moussa5Cristina Antonetti Lamorgese Passeri6Alessia Colosimo7Anna Concetta Berardi8Department of Haematology, Transfusion Medicine and Biotechnologies, Cytofluorimetry and Cell Sorting Service, Ospedale Spirito Santo, 65122 Pescara, ItalyDepartment of Musculoskeletal Disorders, Faculty of Medicine and Surgery, University of Salerno, 84084 Baronissi, ItalyDepartment of Pharmacy, University G. d’Annunzio, 66100 Chieti, ItalyDepartment of Haematology, Transfusion Medicine and Biotechnologies, Cytofluorimetry and Cell Sorting Service, Ospedale Spirito Santo, 65122 Pescara, ItalyDepartment of Medicine, University of Udine, 33100 Udine, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, ItalyDepartment of Haematology, Transfusion Medicine and Biotechnologies, Laboratory of Stem Cells, Ospedale Spirito Santo, 65122 Pescara, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, ItalyDepartment of Haematology, Transfusion Medicine and Biotechnologies, Laboratory of Stem Cells, Ospedale Spirito Santo, 65122 Pescara, ItalyRotator cuff tendon (RCT) disease results from multifactorial mechanisms, in which inflammation plays a key role. Pro-inflammatory cytokines and tendon stem cell/progenitor cells (TSPCs) have been shown to participate in the inflammatory response. However, the underlying molecular mechanism is still not clear. In this study, flow cytometry analyses of different subpopulations of RCT-derived TSPCs demonstrate that after three days of administration, TNFα alone or in combination with IFNγ significantly decreases the percentage of CD146+CD49d+ and CD146+CD49f+ but not CD146+CD109+ TSPCs populations. In parallel, the same pro-inflammatory cytokines upregulate the expression of CD200 in the CD146+ TSPCs population. Additionally, the TNFα/IFNγ combination modulates the protein expression of STAT1, STAT3, and MMP9, but not fibromodulin. At the gene level, <i>IRF1</i>, <i>CAAT (CAAT/EBPbeta)</i>, and <i>DOK2</i> but not <i>NF-κb</i>, <i>TGRF2 (TGFBR2)</i>, and <i>RAS-GAP</i> are modulated. In conclusion, although our study has several important limitations, the results highlight a new potential role of CD200 in regulating inflammation during tendon injuries. In addition, the genes analyzed here might be new potential players in the inflammatory response of TSPCs.https://www.mdpi.com/1422-0067/23/23/15165tendonsrotator cuff diseaseinflammationCD200TNFαIFNγ |
| spellingShingle | Raffaella Giancola Francesco Oliva Marialucia Gallorini Noemi Michetti Clarissa Gissi Fadl Moussa Cristina Antonetti Lamorgese Passeri Alessia Colosimo Anna Concetta Berardi CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model International Journal of Molecular Sciences tendons rotator cuff disease inflammation CD200 TNFα IFNγ |
| title | CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model |
| title_full | CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model |
| title_fullStr | CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model |
| title_full_unstemmed | CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model |
| title_short | CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model |
| title_sort | cd200 as a potential new player in inflammation during rotator cuff tendon injury repair an in vitro model |
| topic | tendons rotator cuff disease inflammation CD200 TNFα IFNγ |
| url | https://www.mdpi.com/1422-0067/23/23/15165 |
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