Proline Spray Relieves the Adverse Effects of Drought on Wheat Flag Leaf Function
Drought stress is one of the key factors restricting crop yield. The beneficial effects of exogenous proline on crop growth under drought stress have been demonstrated in maize, rice, and other crops. However, little is known about its effects on wheat under drought stress. Especially, the water-hol...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/7/957 |
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| author | Huizhen Li Yuan Liu Bo Zhen Mouchao Lv Xinguo Zhou Beibei Yong Qinglin Niu Shenjiao Yang |
| author_facet | Huizhen Li Yuan Liu Bo Zhen Mouchao Lv Xinguo Zhou Beibei Yong Qinglin Niu Shenjiao Yang |
| author_sort | Huizhen Li |
| collection | DOAJ |
| description | Drought stress is one of the key factors restricting crop yield. The beneficial effects of exogenous proline on crop growth under drought stress have been demonstrated in maize, rice, and other crops. However, little is known about its effects on wheat under drought stress. Especially, the water-holding capacity of leaves were overlooked in most studies. Therefore, a barrel experiment was conducted with wheat at two drought levels (severe drought: 45% field capacity, mild drought: 60% field capacity), and three proline-spraying levels (0 mM, 25 mM, and 50 mM). Meanwhile, a control with no stress and no proline application was set. The anatomical features, water-holding capacity, antioxidant capacity, and proline content of flag leaves as well as grain yields were measured. The results showed that drought stress increased the activity of catalase and peroxidase and the content of proline in flag leaves, lessened the content of chlorophyll, deformed leaf veins, and decreased the grain yield. Exogenous proline could regulate the osmotic-regulation substance content, chlorophyll content, antioxidant enzyme activity, water-holding capacity, and tissue structure of wheat flag leaves under drought stress, ultimately alleviating the impact of drought stress on wheat yield. The application of proline (25 mM and 50 mM) increased the yield by 2.88% and 10.81% under mild drought and 33.90% and 52.88% under severe drought compared to wheat without proline spray, respectively. |
| first_indexed | 2024-04-24T10:36:43Z |
| format | Article |
| id | doaj.art-5d3fc7789f9741a2baca7d1835681490 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T10:36:43Z |
| publishDate | 2024-03-01 |
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| series | Plants |
| spelling | doaj.art-5d3fc7789f9741a2baca7d18356814902024-04-12T13:24:43ZengMDPI AGPlants2223-77472024-03-0113795710.3390/plants13070957Proline Spray Relieves the Adverse Effects of Drought on Wheat Flag Leaf FunctionHuizhen Li0Yuan Liu1Bo Zhen2Mouchao Lv3Xinguo Zhou4Beibei Yong5Qinglin Niu6Shenjiao Yang7Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, ChinaInstitute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, ChinaJiangsu Vocational College of Agriculture & Forestry, Jurong 212499, ChinaInstitute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, ChinaInstitute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, ChinaInstitute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, ChinaInstitute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, ChinaInstitute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, ChinaDrought stress is one of the key factors restricting crop yield. The beneficial effects of exogenous proline on crop growth under drought stress have been demonstrated in maize, rice, and other crops. However, little is known about its effects on wheat under drought stress. Especially, the water-holding capacity of leaves were overlooked in most studies. Therefore, a barrel experiment was conducted with wheat at two drought levels (severe drought: 45% field capacity, mild drought: 60% field capacity), and three proline-spraying levels (0 mM, 25 mM, and 50 mM). Meanwhile, a control with no stress and no proline application was set. The anatomical features, water-holding capacity, antioxidant capacity, and proline content of flag leaves as well as grain yields were measured. The results showed that drought stress increased the activity of catalase and peroxidase and the content of proline in flag leaves, lessened the content of chlorophyll, deformed leaf veins, and decreased the grain yield. Exogenous proline could regulate the osmotic-regulation substance content, chlorophyll content, antioxidant enzyme activity, water-holding capacity, and tissue structure of wheat flag leaves under drought stress, ultimately alleviating the impact of drought stress on wheat yield. The application of proline (25 mM and 50 mM) increased the yield by 2.88% and 10.81% under mild drought and 33.90% and 52.88% under severe drought compared to wheat without proline spray, respectively.https://www.mdpi.com/2223-7747/13/7/957antioxidant enzymeanatomic featureplant growth regulatorswater deficitcrop |
| spellingShingle | Huizhen Li Yuan Liu Bo Zhen Mouchao Lv Xinguo Zhou Beibei Yong Qinglin Niu Shenjiao Yang Proline Spray Relieves the Adverse Effects of Drought on Wheat Flag Leaf Function Plants antioxidant enzyme anatomic feature plant growth regulators water deficit crop |
| title | Proline Spray Relieves the Adverse Effects of Drought on Wheat Flag Leaf Function |
| title_full | Proline Spray Relieves the Adverse Effects of Drought on Wheat Flag Leaf Function |
| title_fullStr | Proline Spray Relieves the Adverse Effects of Drought on Wheat Flag Leaf Function |
| title_full_unstemmed | Proline Spray Relieves the Adverse Effects of Drought on Wheat Flag Leaf Function |
| title_short | Proline Spray Relieves the Adverse Effects of Drought on Wheat Flag Leaf Function |
| title_sort | proline spray relieves the adverse effects of drought on wheat flag leaf function |
| topic | antioxidant enzyme anatomic feature plant growth regulators water deficit crop |
| url | https://www.mdpi.com/2223-7747/13/7/957 |
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