GENETIC CHARACTERIZATION OF LF221 ACIDOCINS
Acidocin LF221 A and B are at least two different bacteriocins produced by Lactobacillus acidophilus LF221. In previous research work the N-terminal amino acid (AA) sequence was determined from the purified peptides and probes for both acidocins were prepared as well. Southern hybridization was use...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
1998-12-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/17080 |
| _version_ | 1797390744303960064 |
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| author | Andreja ČANŽEK MAJHENIČ Irena ROGELJ |
| author_facet | Andreja ČANŽEK MAJHENIČ Irena ROGELJ |
| author_sort | Andreja ČANŽEK MAJHENIČ |
| collection | DOAJ |
| description |
Acidocin LF221 A and B are at least two different bacteriocins produced by Lactobacillus acidophilus LF221. In previous research work the N-terminal amino acid (AA) sequence was determined from the purified peptides and probes for both acidocins were prepared as well. Southern hybridization was used to locate structural genes for acidocin LF221 A and B on the genome. Using as a template digested chromosomal DNA of either LF221 or its bac- derivative, identical results were obtained. A possible explanation for this is that bac- mutant still contains the structural genes for both acidocins. When acidocin LF221 A or B were compared to other known bacteriocins, they showed some homology to ThmB peptide of thermophilin 13 or LafX peptide of lactacin F. We assume that LF221 acidocins are novel representatives of the class II bacteriocins.
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| first_indexed | 2024-03-08T23:21:43Z |
| format | Article |
| id | doaj.art-5d404b8d65e144e28b31b927792763fe |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-08T23:21:43Z |
| publishDate | 1998-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-5d404b8d65e144e28b31b927792763fe2023-12-14T22:16:25ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411998-12-01721GENETIC CHARACTERIZATION OF LF221 ACIDOCINSAndreja ČANŽEK MAJHENIČ0Irena ROGELJ1Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 DomžaleUniv. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale Acidocin LF221 A and B are at least two different bacteriocins produced by Lactobacillus acidophilus LF221. In previous research work the N-terminal amino acid (AA) sequence was determined from the purified peptides and probes for both acidocins were prepared as well. Southern hybridization was used to locate structural genes for acidocin LF221 A and B on the genome. Using as a template digested chromosomal DNA of either LF221 or its bac- derivative, identical results were obtained. A possible explanation for this is that bac- mutant still contains the structural genes for both acidocins. When acidocin LF221 A or B were compared to other known bacteriocins, they showed some homology to ThmB peptide of thermophilin 13 or LafX peptide of lactacin F. We assume that LF221 acidocins are novel representatives of the class II bacteriocins. https://journals.uni-lj.si/aas/article/view/17080microbiologybacteriaLactobacillus acidophilusbacteriocinsmolecular geneticscloning |
| spellingShingle | Andreja ČANŽEK MAJHENIČ Irena ROGELJ GENETIC CHARACTERIZATION OF LF221 ACIDOCINS Acta Agriculturae Slovenica microbiology bacteria Lactobacillus acidophilus bacteriocins molecular genetics cloning |
| title | GENETIC CHARACTERIZATION OF LF221 ACIDOCINS |
| title_full | GENETIC CHARACTERIZATION OF LF221 ACIDOCINS |
| title_fullStr | GENETIC CHARACTERIZATION OF LF221 ACIDOCINS |
| title_full_unstemmed | GENETIC CHARACTERIZATION OF LF221 ACIDOCINS |
| title_short | GENETIC CHARACTERIZATION OF LF221 ACIDOCINS |
| title_sort | genetic characterization of lf221 acidocins |
| topic | microbiology bacteria Lactobacillus acidophilus bacteriocins molecular genetics cloning |
| url | https://journals.uni-lj.si/aas/article/view/17080 |
| work_keys_str_mv | AT andrejacanzekmajhenic geneticcharacterizationoflf221acidocins AT irenarogelj geneticcharacterizationoflf221acidocins |