Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses
Synaptic transmission is initiated via spontaneous or action-potential evoked fusion of synaptic vesicles. At excitatory synapses, glutamatergic receptors activated by spontaneous and evoked neurotransmission are segregated. Although inhibitory synapses also transmit signals spontaneously or in resp...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/52852 |
| _version_ | 1811181084991291392 |
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| author | Patricia M Horvath Michelle K Piazza Lisa M Monteggia Ege T Kavalali |
| author_facet | Patricia M Horvath Michelle K Piazza Lisa M Monteggia Ege T Kavalali |
| author_sort | Patricia M Horvath |
| collection | DOAJ |
| description | Synaptic transmission is initiated via spontaneous or action-potential evoked fusion of synaptic vesicles. At excitatory synapses, glutamatergic receptors activated by spontaneous and evoked neurotransmission are segregated. Although inhibitory synapses also transmit signals spontaneously or in response to action potentials, they differ from excitatory synapses in both structure and function. Therefore, we hypothesized that inhibitory synapses may have different organizing principles. We report picrotoxin, a GABAAR antagonist, blocks neurotransmission in a use-dependent manner at rat hippocampal synapses and therefore can be used to interrogate synaptic properties. Using this tool, we uncovered partial segregation of inhibitory spontaneous and evoked neurotransmission. We found up to 40% of the evoked response is mediated through GABAARs which are only activated by evoked neurotransmission. These data indicate GABAergic spontaneous and evoked neurotransmission processes are partially non-overlapping, suggesting they may serve divergent roles in neuronal signaling. |
| first_indexed | 2024-04-11T09:13:52Z |
| format | Article |
| id | doaj.art-5d42b65ad1034437a3ace174f99c440f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:13:52Z |
| publishDate | 2020-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5d42b65ad1034437a3ace174f99c440f2022-12-22T04:32:25ZengeLife Sciences Publications LtdeLife2050-084X2020-05-01910.7554/eLife.52852Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapsesPatricia M Horvath0https://orcid.org/0000-0001-9969-8637Michelle K Piazza1https://orcid.org/0000-0002-7852-3456Lisa M Monteggia2https://orcid.org/0000-0003-0018-501XEge T Kavalali3https://orcid.org/0000-0003-1777-227XDepartment of Pharmacology, Vanderbilt University, Nashville, United States; Department of Neuroscience, the University of Texas Southwestern Medical Center, Dallas, United StatesVanderbilt Brain Institute, Vanderbilt University, Nashville, United States; Neuroscience Program, Vanderbilt University, Nashville, United StatesDepartment of Pharmacology, Vanderbilt University, Nashville, United States; Vanderbilt Brain Institute, Vanderbilt University, Nashville, United StatesDepartment of Pharmacology, Vanderbilt University, Nashville, United States; Vanderbilt Brain Institute, Vanderbilt University, Nashville, United StatesSynaptic transmission is initiated via spontaneous or action-potential evoked fusion of synaptic vesicles. At excitatory synapses, glutamatergic receptors activated by spontaneous and evoked neurotransmission are segregated. Although inhibitory synapses also transmit signals spontaneously or in response to action potentials, they differ from excitatory synapses in both structure and function. Therefore, we hypothesized that inhibitory synapses may have different organizing principles. We report picrotoxin, a GABAAR antagonist, blocks neurotransmission in a use-dependent manner at rat hippocampal synapses and therefore can be used to interrogate synaptic properties. Using this tool, we uncovered partial segregation of inhibitory spontaneous and evoked neurotransmission. We found up to 40% of the evoked response is mediated through GABAARs which are only activated by evoked neurotransmission. These data indicate GABAergic spontaneous and evoked neurotransmission processes are partially non-overlapping, suggesting they may serve divergent roles in neuronal signaling.https://elifesciences.org/articles/52852spontaneous releaseGABAergic neurotransmissionpicrotoxin |
| spellingShingle | Patricia M Horvath Michelle K Piazza Lisa M Monteggia Ege T Kavalali Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses eLife spontaneous release GABAergic neurotransmission picrotoxin |
| title | Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses |
| title_full | Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses |
| title_fullStr | Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses |
| title_full_unstemmed | Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses |
| title_short | Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses |
| title_sort | spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses |
| topic | spontaneous release GABAergic neurotransmission picrotoxin |
| url | https://elifesciences.org/articles/52852 |
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