Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4V
Loosening of orthodontic and orthopedic implants is a critical and common clinical problem. To minimize the numbers of revision surgeries due to peri-implant inflammation or insufficient osseointegration, developments of new implant manufacturing strategies are indicated. Ultrafast laser surface tex...
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| Language: | English |
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MDPI AG
2020-11-01
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| Online Access: | https://www.mdpi.com/1996-1944/13/23/5342 |
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| author | Luiz Schweitzer Alexandre Cunha Thiago Pereira Kerstin Mika Ana Maria Botelho do Rego Ana Maria Ferraria Heinz Kieburg Sven Geissler Eckart Uhlmann Janosch Schoon |
| author_facet | Luiz Schweitzer Alexandre Cunha Thiago Pereira Kerstin Mika Ana Maria Botelho do Rego Ana Maria Ferraria Heinz Kieburg Sven Geissler Eckart Uhlmann Janosch Schoon |
| author_sort | Luiz Schweitzer |
| collection | DOAJ |
| description | Loosening of orthodontic and orthopedic implants is a critical and common clinical problem. To minimize the numbers of revision surgeries due to peri-implant inflammation or insufficient osseointegration, developments of new implant manufacturing strategies are indicated. Ultrafast laser surface texturing is a promising contact-free technology to modify the physicochemical properties of surfaces toward an anti-infectious functionalization. This work aims to texture Ti6Al4V surfaces with ultraviolet (UV) and green (GR) radiation for the manufacturing of laser-induced periodic surface structures (LIPSS). The assessment of these surface modifications addresses key aspects of topography, morphology and chemical composition. Human primary mesenchymal stromal cells (hMSCs) were cultured on laser-textured and polished Ti6Al4V to characterize the surfaces in terms of their in vitro biocompatibility, cytotoxicity, and metal release. The outcomes of the in vitro experiment show the successful culture of hMSCs on textured Ti6Al4V surfaces developed within this work. Cells cultured on LIPSS surfaces were not compromised in terms of their viability if compared to polished surfaces. Yet, the hMSC culture on UV-LIPSS show significantly lower lactate dehydrogenase and titanium release into the supernatant compared to polished. Thus, the presented surface modification can be a promising approach for future applications in orthodontics and orthopedics. |
| first_indexed | 2024-03-10T14:34:56Z |
| format | Article |
| id | doaj.art-5d43db936cdc48eb988371ba99f523be |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T14:34:56Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-5d43db936cdc48eb988371ba99f523be2023-11-20T22:18:25ZengMDPI AGMaterials1996-19442020-11-011323534210.3390/ma13235342Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4VLuiz Schweitzer0Alexandre Cunha1Thiago Pereira2Kerstin Mika3Ana Maria Botelho do Rego4Ana Maria Ferraria5Heinz Kieburg6Sven Geissler7Eckart Uhlmann8Janosch Schoon9Fraunhofer Institute for Production Systems and Design Technology, Pascalstr. 8-9, 10587 Berlin, GermanySENAI Innovation Institute in Manufacturing Systems and Laser Processing, Rua Arno Waldemar Döhler 308, Joinville, 89218-153 Santa Catarina, BrazilSENAI Innovation Institute in Manufacturing Systems and Laser Processing, Rua Arno Waldemar Döhler 308, Joinville, 89218-153 Santa Catarina, BrazilJulius Wolff Institute, Charité Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyBSIRG, Departamento de Engenharia Química, iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, PortugalBSIRG, Departamento de Engenharia Química, iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, PortugalLaser-Mikrotechnologie Dr. Kieburg, James-Frank-Str. 15, 12489 Berlin, GermanyJulius Wolff Institute, Charité Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyFraunhofer Institute for Production Systems and Design Technology, Pascalstr. 8-9, 10587 Berlin, GermanyDepartment of Orthopedics and Orthopedic Surgery, University Medicine Greifswald, 17475 Greifswald, GermanyLoosening of orthodontic and orthopedic implants is a critical and common clinical problem. To minimize the numbers of revision surgeries due to peri-implant inflammation or insufficient osseointegration, developments of new implant manufacturing strategies are indicated. Ultrafast laser surface texturing is a promising contact-free technology to modify the physicochemical properties of surfaces toward an anti-infectious functionalization. This work aims to texture Ti6Al4V surfaces with ultraviolet (UV) and green (GR) radiation for the manufacturing of laser-induced periodic surface structures (LIPSS). The assessment of these surface modifications addresses key aspects of topography, morphology and chemical composition. Human primary mesenchymal stromal cells (hMSCs) were cultured on laser-textured and polished Ti6Al4V to characterize the surfaces in terms of their in vitro biocompatibility, cytotoxicity, and metal release. The outcomes of the in vitro experiment show the successful culture of hMSCs on textured Ti6Al4V surfaces developed within this work. Cells cultured on LIPSS surfaces were not compromised in terms of their viability if compared to polished surfaces. Yet, the hMSC culture on UV-LIPSS show significantly lower lactate dehydrogenase and titanium release into the supernatant compared to polished. Thus, the presented surface modification can be a promising approach for future applications in orthodontics and orthopedics.https://www.mdpi.com/1996-1944/13/23/5342Ti6Al4Vlaser surface texturingLIPSSbiocompatibilitycytotoxicity |
| spellingShingle | Luiz Schweitzer Alexandre Cunha Thiago Pereira Kerstin Mika Ana Maria Botelho do Rego Ana Maria Ferraria Heinz Kieburg Sven Geissler Eckart Uhlmann Janosch Schoon Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4V Materials Ti6Al4V laser surface texturing LIPSS biocompatibility cytotoxicity |
| title | Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4V |
| title_full | Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4V |
| title_fullStr | Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4V |
| title_full_unstemmed | Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4V |
| title_short | Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4V |
| title_sort | preclinical in vitro assessment of submicron scale laser surface texturing on ti6al4v |
| topic | Ti6Al4V laser surface texturing LIPSS biocompatibility cytotoxicity |
| url | https://www.mdpi.com/1996-1944/13/23/5342 |
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