Correction of morphofunctional changes in mice thymus and spleen by Rhaponticum uniflorum (L.) DC. extract at cyclophosphamide immunosupression

The aim of the study is to evaluate the effect of R. uniflorum dry extract on morphofunctional state of mice thymus and spleen at cyclophosphamide immunosuppression.Materials and methods. The experiments were carried out on F1 (CBAxC57Bl/6) mice 18–20 g of weight. Immune deficiency was simulated by...

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Main Authors: Ya. G. Razuvaeva, V. B. Khobrakova, Yu. A. Tugarina, D. N. Olennikov
Format: Article
Language:Russian
Published: Scientific Сentre for Family Health and Human Reproduction Problems 2022-12-01
Series:Acta Biomedica Scientifica
Subjects:
Online Access:https://www.actabiomedica.ru/jour/article/view/3831
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author Ya. G. Razuvaeva
V. B. Khobrakova
Yu. A. Tugarina
D. N. Olennikov
author_facet Ya. G. Razuvaeva
V. B. Khobrakova
Yu. A. Tugarina
D. N. Olennikov
author_sort Ya. G. Razuvaeva
collection DOAJ
description The aim of the study is to evaluate the effect of R. uniflorum dry extract on morphofunctional state of mice thymus and spleen at cyclophosphamide immunosuppression.Materials and methods. The experiments were carried out on F1 (CBAxC57Bl/6) mice 18–20 g of weight. Immune deficiency was simulated by a single intraperitoneal introduction of cyclophosphamide in the dose 250 mg/kg. R. uniflorum dry extract in the dose 100 mg/kg was administered per os for 14 days. The thymus and spleen were fixed for histological studies on day 16. The thymus total area, the cortex and medulla area, the connective tissue capsule thickness, the spleen white and red pulp area were used to measure on micropreparations stained with hematoxylineosin and azure-eosin. The cell density and the cellular composition were calculated in the thymus cortical substance.Results. The R. uniflorum extract increased the thymus total area and the corticomedullary index by 37 and 26 % (p ≤ 0.05), respectively. The cells density increased in the cortical substance, it decreased in the medulla, as a result “layer inversion” is not observed in any animal of the experimental group. The number of proliferating lymphocytes, blasts and large lymphocytes increased; the number of destructive cells and macrophages decreased in the cortical substance. The spleen white pulp relative volume was 39 % higher than in the control.Conclusions. The R. uniflorum dry extract had an immunocorrective effect on the thymus and spleen morphofunctional state at cyclophosphamid immunosuppression.
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spelling doaj.art-5d4424bd083946678b8e9439597777492025-01-27T16:49:01ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962022-12-0175-211412110.29413/ABS.2022-7.5-2.122442Correction of morphofunctional changes in mice thymus and spleen by Rhaponticum uniflorum (L.) DC. extract at cyclophosphamide immunosupressionYa. G. Razuvaeva0V. B. Khobrakova1Yu. A. Tugarina2D. N. Olennikov3Institute of General and Experimental biology of the Siberian Branch of the Russian Academy of SciencesInstitute of General and Experimental biology of the Siberian Branch of the Russian Academy of SciencesInstitute of General and Experimental biology of the Siberian Branch of the Russian Academy of SciencesInstitute of General and Experimental biology of the Siberian Branch of the Russian Academy of SciencesThe aim of the study is to evaluate the effect of R. uniflorum dry extract on morphofunctional state of mice thymus and spleen at cyclophosphamide immunosuppression.Materials and methods. The experiments were carried out on F1 (CBAxC57Bl/6) mice 18–20 g of weight. Immune deficiency was simulated by a single intraperitoneal introduction of cyclophosphamide in the dose 250 mg/kg. R. uniflorum dry extract in the dose 100 mg/kg was administered per os for 14 days. The thymus and spleen were fixed for histological studies on day 16. The thymus total area, the cortex and medulla area, the connective tissue capsule thickness, the spleen white and red pulp area were used to measure on micropreparations stained with hematoxylineosin and azure-eosin. The cell density and the cellular composition were calculated in the thymus cortical substance.Results. The R. uniflorum extract increased the thymus total area and the corticomedullary index by 37 and 26 % (p ≤ 0.05), respectively. The cells density increased in the cortical substance, it decreased in the medulla, as a result “layer inversion” is not observed in any animal of the experimental group. The number of proliferating lymphocytes, blasts and large lymphocytes increased; the number of destructive cells and macrophages decreased in the cortical substance. The spleen white pulp relative volume was 39 % higher than in the control.Conclusions. The R. uniflorum dry extract had an immunocorrective effect on the thymus and spleen morphofunctional state at cyclophosphamid immunosuppression.https://www.actabiomedica.ru/jour/article/view/3831rhaponticum uniflorum (l.) dc.dry extractthymusspleencyclophosphamideimmunosuppression
spellingShingle Ya. G. Razuvaeva
V. B. Khobrakova
Yu. A. Tugarina
D. N. Olennikov
Correction of morphofunctional changes in mice thymus and spleen by Rhaponticum uniflorum (L.) DC. extract at cyclophosphamide immunosupression
Acta Biomedica Scientifica
rhaponticum uniflorum (l.) dc.
dry extract
thymus
spleen
cyclophosphamide
immunosuppression
title Correction of morphofunctional changes in mice thymus and spleen by Rhaponticum uniflorum (L.) DC. extract at cyclophosphamide immunosupression
title_full Correction of morphofunctional changes in mice thymus and spleen by Rhaponticum uniflorum (L.) DC. extract at cyclophosphamide immunosupression
title_fullStr Correction of morphofunctional changes in mice thymus and spleen by Rhaponticum uniflorum (L.) DC. extract at cyclophosphamide immunosupression
title_full_unstemmed Correction of morphofunctional changes in mice thymus and spleen by Rhaponticum uniflorum (L.) DC. extract at cyclophosphamide immunosupression
title_short Correction of morphofunctional changes in mice thymus and spleen by Rhaponticum uniflorum (L.) DC. extract at cyclophosphamide immunosupression
title_sort correction of morphofunctional changes in mice thymus and spleen by rhaponticum uniflorum l dc extract at cyclophosphamide immunosupression
topic rhaponticum uniflorum (l.) dc.
dry extract
thymus
spleen
cyclophosphamide
immunosuppression
url https://www.actabiomedica.ru/jour/article/view/3831
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AT yuatugarina correctionofmorphofunctionalchangesinmicethymusandspleenbyrhaponticumuniflorumldcextractatcyclophosphamideimmunosupression
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