The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR Genes
Scab caused by the pathogen Venturia inaequalis is considered the most important fungal disease of cultivated apple (Malus × domestica Borkh.). In all, 16 monogenic resistances against scab have been found in different Malus spp. and some of them are currently used in apple breeding for scab-resista...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2010-05-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-5-0608 |
| _version_ | 1811261364068417536 |
|---|---|
| author | Paolo Galli Andrea Patocchi Giovanni Antonio Lodovico Broggini Cesare Gessler |
| author_facet | Paolo Galli Andrea Patocchi Giovanni Antonio Lodovico Broggini Cesare Gessler |
| author_sort | Paolo Galli |
| collection | DOAJ |
| description | Scab caused by the pathogen Venturia inaequalis is considered the most important fungal disease of cultivated apple (Malus × domestica Borkh.). In all, 16 monogenic resistances against scab have been found in different Malus spp. and some of them are currently used in apple breeding for scab-resistant cultivars. However, the self incompatibility and the long generation time of Malus spp. together with the high standards of fruit quality demanded from the fresh market render the breeding of high-quality cultivars in apple a long and expensive task. Therefore, the cloning of disease resistance genes and the use of the cloned genes for the transformation of high-quality apple cultivars could be an approach to solve these drawbacks. We report the construction of a bacterial artificial chromosome (BAC) contig spanning the Rvi15 (Vr2) apple scab resistance locus using two GMAL 2473 BAC libraries. A single BAC clone of the contig was sufficient to span the resistance locus. The BAC clone was completely sequenced, allowing identification of a sequence of 48.6 kb going from the two closest markers (ARGH17 and 77G20RP) bracketing Rvi15 (Vr2). Analysis of the 48.6-kb sequence revealed the presence of three putative genes characterized by a Toll and mammalian interleukin-1 receptor protein nucleotide-binding site leucine-rich repeat structure. All three genes were found to be transcribed. |
| first_indexed | 2024-04-12T19:02:09Z |
| format | Article |
| id | doaj.art-5d44587b761646b291b04f956b2044c8 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T19:02:09Z |
| publishDate | 2010-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-5d44587b761646b291b04f956b2044c82022-12-22T03:20:07ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062010-05-0123560861710.1094/MPMI-23-5-0608The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR GenesPaolo GalliAndrea PatocchiGiovanni Antonio Lodovico BrogginiCesare GesslerScab caused by the pathogen Venturia inaequalis is considered the most important fungal disease of cultivated apple (Malus × domestica Borkh.). In all, 16 monogenic resistances against scab have been found in different Malus spp. and some of them are currently used in apple breeding for scab-resistant cultivars. However, the self incompatibility and the long generation time of Malus spp. together with the high standards of fruit quality demanded from the fresh market render the breeding of high-quality cultivars in apple a long and expensive task. Therefore, the cloning of disease resistance genes and the use of the cloned genes for the transformation of high-quality apple cultivars could be an approach to solve these drawbacks. We report the construction of a bacterial artificial chromosome (BAC) contig spanning the Rvi15 (Vr2) apple scab resistance locus using two GMAL 2473 BAC libraries. A single BAC clone of the contig was sufficient to span the resistance locus. The BAC clone was completely sequenced, allowing identification of a sequence of 48.6 kb going from the two closest markers (ARGH17 and 77G20RP) bracketing Rvi15 (Vr2). Analysis of the 48.6-kb sequence revealed the presence of three putative genes characterized by a Toll and mammalian interleukin-1 receptor protein nucleotide-binding site leucine-rich repeat structure. All three genes were found to be transcribed.https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-5-0608 |
| spellingShingle | Paolo Galli Andrea Patocchi Giovanni Antonio Lodovico Broggini Cesare Gessler The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR Genes Molecular Plant-Microbe Interactions |
| title | The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR Genes |
| title_full | The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR Genes |
| title_fullStr | The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR Genes |
| title_full_unstemmed | The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR Genes |
| title_short | The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR Genes |
| title_sort | rvi15 vr2 apple scab resistance locus contains three tir nbs lrr genes |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-5-0608 |
| work_keys_str_mv | AT paologalli thervi15vr2applescabresistancelocuscontainsthreetirnbslrrgenes AT andreapatocchi thervi15vr2applescabresistancelocuscontainsthreetirnbslrrgenes AT giovanniantoniolodovicobroggini thervi15vr2applescabresistancelocuscontainsthreetirnbslrrgenes AT cesaregessler thervi15vr2applescabresistancelocuscontainsthreetirnbslrrgenes AT paologalli rvi15vr2applescabresistancelocuscontainsthreetirnbslrrgenes AT andreapatocchi rvi15vr2applescabresistancelocuscontainsthreetirnbslrrgenes AT giovanniantoniolodovicobroggini rvi15vr2applescabresistancelocuscontainsthreetirnbslrrgenes AT cesaregessler rvi15vr2applescabresistancelocuscontainsthreetirnbslrrgenes |