Regulation of actin cytoskeleton via photolithographic micropatterning
Actin cytoskeleton plays crucial roles in various cellular functions. Extracellular matrix (ECM) can modulate cell morphology by remodeling the internal cytoskeleton. To define how geometry of ECM regulates the organization of actin cytoskeleton, we plated individual NIH 3T3 cells on micropatterned...
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2023-03-01
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| Series: | Journal of Innovative Optical Health Sciences |
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| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545822440059 |
| _version_ | 1797865830492405760 |
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| author | Fulin Xing Haimei Zhang Mengyu Li Hao Dong Xuehe Ma Shiyu Deng Fen Hu Imshik Lee Leiting Pan Jingjun Xu |
| author_facet | Fulin Xing Haimei Zhang Mengyu Li Hao Dong Xuehe Ma Shiyu Deng Fen Hu Imshik Lee Leiting Pan Jingjun Xu |
| author_sort | Fulin Xing |
| collection | DOAJ |
| description | Actin cytoskeleton plays crucial roles in various cellular functions. Extracellular matrix (ECM) can modulate cell morphology by remodeling the internal cytoskeleton. To define how geometry of ECM regulates the organization of actin cytoskeleton, we plated individual NIH 3T3 cells on micropatterned substrates with distinct shapes and sizes. It was found that the stress fibers could form along the nonadhesive edges of T-shaped pattern, but were absent from the opening edge of V-shaped pattern, indicating that the organization of actin cytoskeleton was dependent on the mechanical environment. Furthermore, a secondary actin ring was observed on 50[Formula: see text][Formula: see text]m circular pattern while did not appear on 30[Formula: see text][Formula: see text]m and 40[Formula: see text][Formula: see text]m pattern, showing a size-dependent organization of actin cytoskeleton. Finally, osteoblasts, MDCK and A549 cells exhibited distinct organization of actin cytoskeleton on T-shaped pattern, suggesting a cell-type specificity in arrangement of actin cytoskeleton. Together, our findings brought novel insight into the organization of actin cytoskeleton on micropatterned environments. |
| first_indexed | 2024-04-09T23:15:17Z |
| format | Article |
| id | doaj.art-5d450e7c176d4e85817907a2d333b03b |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-04-09T23:15:17Z |
| publishDate | 2023-03-01 |
| publisher | World Scientific Publishing |
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| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-5d450e7c176d4e85817907a2d333b03b2023-03-22T10:03:09ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-03-01160210.1142/S1793545822440059Regulation of actin cytoskeleton via photolithographic micropatterningFulin Xing0Haimei Zhang1Mengyu Li2Hao Dong3Xuehe Ma4Shiyu Deng5Fen Hu6Imshik Lee7Leiting Pan8Jingjun Xu9The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071 P. R. ChinaActin cytoskeleton plays crucial roles in various cellular functions. Extracellular matrix (ECM) can modulate cell morphology by remodeling the internal cytoskeleton. To define how geometry of ECM regulates the organization of actin cytoskeleton, we plated individual NIH 3T3 cells on micropatterned substrates with distinct shapes and sizes. It was found that the stress fibers could form along the nonadhesive edges of T-shaped pattern, but were absent from the opening edge of V-shaped pattern, indicating that the organization of actin cytoskeleton was dependent on the mechanical environment. Furthermore, a secondary actin ring was observed on 50[Formula: see text][Formula: see text]m circular pattern while did not appear on 30[Formula: see text][Formula: see text]m and 40[Formula: see text][Formula: see text]m pattern, showing a size-dependent organization of actin cytoskeleton. Finally, osteoblasts, MDCK and A549 cells exhibited distinct organization of actin cytoskeleton on T-shaped pattern, suggesting a cell-type specificity in arrangement of actin cytoskeleton. Together, our findings brought novel insight into the organization of actin cytoskeleton on micropatterned environments.https://www.worldscientific.com/doi/10.1142/S1793545822440059Actin cytoskeletonphotolithographymicropatterningextracellular matrix |
| spellingShingle | Fulin Xing Haimei Zhang Mengyu Li Hao Dong Xuehe Ma Shiyu Deng Fen Hu Imshik Lee Leiting Pan Jingjun Xu Regulation of actin cytoskeleton via photolithographic micropatterning Journal of Innovative Optical Health Sciences Actin cytoskeleton photolithography micropatterning extracellular matrix |
| title | Regulation of actin cytoskeleton via photolithographic micropatterning |
| title_full | Regulation of actin cytoskeleton via photolithographic micropatterning |
| title_fullStr | Regulation of actin cytoskeleton via photolithographic micropatterning |
| title_full_unstemmed | Regulation of actin cytoskeleton via photolithographic micropatterning |
| title_short | Regulation of actin cytoskeleton via photolithographic micropatterning |
| title_sort | regulation of actin cytoskeleton via photolithographic micropatterning |
| topic | Actin cytoskeleton photolithography micropatterning extracellular matrix |
| url | https://www.worldscientific.com/doi/10.1142/S1793545822440059 |
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