Evaluation of the immunogenicity of Schistosoma mansoni egg surface
Abstract INTRODUCTION Immunogenicity of Schistosoma mansoni egg surface was examined to determine whether intact eggshells have lower antigenicity than ruptured eggs. METHODS: Swiss Webster mice were inoculated with intact or ultrasonicated S. mansoni eggs isolated from infected human feces. Mic...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000500652&lng=en&tlng=en |
| _version_ | 1828338564803330048 |
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| author | Renata Russo Frasca Candido Timothy Guy St. Pierre Malcolm Kenneth Jones Carlos Graeff-Teixeira |
| author_facet | Renata Russo Frasca Candido Timothy Guy St. Pierre Malcolm Kenneth Jones Carlos Graeff-Teixeira |
| author_sort | Renata Russo Frasca Candido |
| collection | DOAJ |
| description | Abstract INTRODUCTION Immunogenicity of Schistosoma mansoni egg surface was examined to determine whether intact eggshells have lower antigenicity than ruptured eggs. METHODS: Swiss Webster mice were inoculated with intact or ultrasonicated S. mansoni eggs isolated from infected human feces. Mice were separated into four groups of six animals each and immunizations were performed approximately every 20 days during a 60-day period. Groups 1-4 were administered with saline solution, sonicated eggs with Freund’s adjuvant, sonicated eggs without Freund’s adjuvant, and intact eggs, respectively. IgG humoral immune response was assessed by ELISA using Soluble Egg Antigen produced from eggs isolated from the livers of infected mice. RESULTS Sonicated eggs co-administered with adjuvant induced the highest humoral response at 58 days, which was 11.9-fold (95% CI 6.2-17.5) greater than the response induced by saline solution. Sonicated eggs without adjuvant induced a 4.3-fold stronger response (95% CI 2.4-6.2) than normal saline. Intact eggs induced humoral response that was nominally twice stronger (95% CI 0.8-3.2) than that induced by normal saline but the effect did not reach statistical significance. CONCLUSIONS Soluble antigens are not abundant on the surface of S. mansoni eggs and/or are not secreted in sufficient quantities to induce a significant immune response to intact eggs. Assuming that isolation procedures had not damaged the eggs used for inoculation, our observations suggest that intact eggs either do not induce a significant immune response or, if they do, the mechanism involves insoluble antigens from the egg surface. |
| first_indexed | 2024-04-13T22:30:36Z |
| format | Article |
| id | doaj.art-5d4879f9eeb148cc93bbb634394e93c4 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-04-13T22:30:36Z |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-5d4879f9eeb148cc93bbb634394e93c42022-12-22T02:26:57ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-984950565265710.1590/0037-8682-0040-2017S0037-86822017000500652Evaluation of the immunogenicity of Schistosoma mansoni egg surfaceRenata Russo Frasca CandidoTimothy Guy St. PierreMalcolm Kenneth JonesCarlos Graeff-TeixeiraAbstract INTRODUCTION Immunogenicity of Schistosoma mansoni egg surface was examined to determine whether intact eggshells have lower antigenicity than ruptured eggs. METHODS: Swiss Webster mice were inoculated with intact or ultrasonicated S. mansoni eggs isolated from infected human feces. Mice were separated into four groups of six animals each and immunizations were performed approximately every 20 days during a 60-day period. Groups 1-4 were administered with saline solution, sonicated eggs with Freund’s adjuvant, sonicated eggs without Freund’s adjuvant, and intact eggs, respectively. IgG humoral immune response was assessed by ELISA using Soluble Egg Antigen produced from eggs isolated from the livers of infected mice. RESULTS Sonicated eggs co-administered with adjuvant induced the highest humoral response at 58 days, which was 11.9-fold (95% CI 6.2-17.5) greater than the response induced by saline solution. Sonicated eggs without adjuvant induced a 4.3-fold stronger response (95% CI 2.4-6.2) than normal saline. Intact eggs induced humoral response that was nominally twice stronger (95% CI 0.8-3.2) than that induced by normal saline but the effect did not reach statistical significance. CONCLUSIONS Soluble antigens are not abundant on the surface of S. mansoni eggs and/or are not secreted in sufficient quantities to induce a significant immune response to intact eggs. Assuming that isolation procedures had not damaged the eggs used for inoculation, our observations suggest that intact eggs either do not induce a significant immune response or, if they do, the mechanism involves insoluble antigens from the egg surface.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000500652&lng=en&tlng=enSchistosomiasisEgg antigensImmune response |
| spellingShingle | Renata Russo Frasca Candido Timothy Guy St. Pierre Malcolm Kenneth Jones Carlos Graeff-Teixeira Evaluation of the immunogenicity of Schistosoma mansoni egg surface Revista da Sociedade Brasileira de Medicina Tropical Schistosomiasis Egg antigens Immune response |
| title | Evaluation of the immunogenicity of Schistosoma mansoni egg surface |
| title_full | Evaluation of the immunogenicity of Schistosoma mansoni egg surface |
| title_fullStr | Evaluation of the immunogenicity of Schistosoma mansoni egg surface |
| title_full_unstemmed | Evaluation of the immunogenicity of Schistosoma mansoni egg surface |
| title_short | Evaluation of the immunogenicity of Schistosoma mansoni egg surface |
| title_sort | evaluation of the immunogenicity of schistosoma mansoni egg surface |
| topic | Schistosomiasis Egg antigens Immune response |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000500652&lng=en&tlng=en |
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