Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial Sources
Fucoidan is a fucose-rich sulfated polysaccharide typically found in the cell wall of marine algae but also recently isolated from terrestrial sources. Due to a variety of biological activities, including antioxidant properties, fucoidan exhibits an attractive therapeutic potential against a wide ar...
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MDPI AG
2021-07-01
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author | Zeinab El Rashed Giulio Lupidi Elena Grasselli Laura Canesi Hala Khalifeh Ilaria Demori |
author_facet | Zeinab El Rashed Giulio Lupidi Elena Grasselli Laura Canesi Hala Khalifeh Ilaria Demori |
author_sort | Zeinab El Rashed |
collection | DOAJ |
description | Fucoidan is a fucose-rich sulfated polysaccharide typically found in the cell wall of marine algae but also recently isolated from terrestrial sources. Due to a variety of biological activities, including antioxidant properties, fucoidan exhibits an attractive therapeutic potential against a wide array of metabolic diseases associated with oxidative stress. We used FTIR, <sup>1</sup>H NMR and <sup>13</sup>C NMR spectroscopy to investigate the structural features of a fucoidan fraction extracted from the brown alga <i>Cystoseira compressa</i> (CYS). The antioxidant potential of CYS was measured by DPPH, ABTS and FRAP assays, which revealed a radical scavenging capacity that was confirmed in in vitro cellular models of hepatic and endothelial cells. The same antioxidant effects were observed for another fucoidan fraction previously identified in the terrestrial tree <i>Eucalyptus globulus</i> (EUC). Moreover, in hepatic cells, CYS and EUC exhibited a significant antisteatotic action, being able to reduce intracellular triglyceride content through the regulation of key genes of hepatic lipid metabolism. EUC exerted stronger antioxidant and antisteatotic effects as compared to CYS, suggesting that both marine and terrestrial sources should be considered for fucoidan extraction and therapeutic applications. |
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issn | 1420-3049 |
language | English |
last_indexed | 2024-03-10T09:11:04Z |
publishDate | 2021-07-01 |
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spelling | doaj.art-5d48a70d6746469e90cd77fd4694cc7c2023-11-22T05:58:32ZengMDPI AGMolecules1420-30492021-07-012615446710.3390/molecules26154467Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial SourcesZeinab El Rashed0Giulio Lupidi1Elena Grasselli2Laura Canesi3Hala Khalifeh4Ilaria Demori5Department of Earth, Environmental and Life Sciences (DISTAV), University of Genoa, 16132 Genoa, ItalySchool of Pharmacy, University of Camerino, 62032 Camerino, ItalyDepartment of Earth, Environmental and Life Sciences (DISTAV), University of Genoa, 16132 Genoa, ItalyDepartment of Earth, Environmental and Life Sciences (DISTAV), University of Genoa, 16132 Genoa, ItalyRammal Rammal Laboratory (ATAC Group), Faculty of Sciences I, Lebanese University, Beirut 1003, LebanonDepartment of Earth, Environmental and Life Sciences (DISTAV), University of Genoa, 16132 Genoa, ItalyFucoidan is a fucose-rich sulfated polysaccharide typically found in the cell wall of marine algae but also recently isolated from terrestrial sources. Due to a variety of biological activities, including antioxidant properties, fucoidan exhibits an attractive therapeutic potential against a wide array of metabolic diseases associated with oxidative stress. We used FTIR, <sup>1</sup>H NMR and <sup>13</sup>C NMR spectroscopy to investigate the structural features of a fucoidan fraction extracted from the brown alga <i>Cystoseira compressa</i> (CYS). The antioxidant potential of CYS was measured by DPPH, ABTS and FRAP assays, which revealed a radical scavenging capacity that was confirmed in in vitro cellular models of hepatic and endothelial cells. The same antioxidant effects were observed for another fucoidan fraction previously identified in the terrestrial tree <i>Eucalyptus globulus</i> (EUC). Moreover, in hepatic cells, CYS and EUC exhibited a significant antisteatotic action, being able to reduce intracellular triglyceride content through the regulation of key genes of hepatic lipid metabolism. EUC exerted stronger antioxidant and antisteatotic effects as compared to CYS, suggesting that both marine and terrestrial sources should be considered for fucoidan extraction and therapeutic applications.https://www.mdpi.com/1420-3049/26/15/4467<i>Cystoseira compressa</i><i>Eucalyptus globulus</i>fucoidanantioxidantantisteatoticNAFLD |
spellingShingle | Zeinab El Rashed Giulio Lupidi Elena Grasselli Laura Canesi Hala Khalifeh Ilaria Demori Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial Sources Molecules <i>Cystoseira compressa</i> <i>Eucalyptus globulus</i> fucoidan antioxidant antisteatotic NAFLD |
title | Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial Sources |
title_full | Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial Sources |
title_fullStr | Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial Sources |
title_full_unstemmed | Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial Sources |
title_short | Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial Sources |
title_sort | antioxidant and antisteatotic activities of fucoidan fractions from marine and terrestrial sources |
topic | <i>Cystoseira compressa</i> <i>Eucalyptus globulus</i> fucoidan antioxidant antisteatotic NAFLD |
url | https://www.mdpi.com/1420-3049/26/15/4467 |
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