KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway
Abstract Background Cervical cancer is a kind of malignant gynecological tumor. The first choice for treating cervical cancer is still a combination of surgery and chemoradiotherapy, but the 5-year survival rate remains poor. Therefore, researchers are trying to find new ways to diagnose and treat c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-022-02585-3 |
| _version_ | 1818523840175669248 |
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| author | Jie Zhang Gulimire Buranjiang Zuohelaguli Mutalifu Hua Jin Liyan Yao |
| author_facet | Jie Zhang Gulimire Buranjiang Zuohelaguli Mutalifu Hua Jin Liyan Yao |
| author_sort | Jie Zhang |
| collection | DOAJ |
| description | Abstract Background Cervical cancer is a kind of malignant gynecological tumor. The first choice for treating cervical cancer is still a combination of surgery and chemoradiotherapy, but the 5-year survival rate remains poor. Therefore, researchers are trying to find new ways to diagnose and treat cervical cancer early. Methods The expression level of KIF14 in cells and tissues was determined via qRT–PCR. The ability of the cells to proliferate, migrate, and invade was examined using CCK-8 assay kits, colony formation assays, and Transwell chambers. The expression levels of Cyclin D1, Cyclin B1, p21, and p27 were also detected using western blot assays. Results The results suggested that p27 is a key regulatory factor in the KIF14-mediated regulation of the cell cycle. In addition, KIF14 knockdown promotes malignancy in cervical cancer cells by inhibiting p27 degradation, resulting in cell cycle arrest. Conclusions KIF14 is an oncogene in cervical cancer, and knocking down KIF14 causes cell cycle arrest by inhibiting p27 degradation, thus affecting cell viability, proliferation, and migration. These results provide a potential therapeutic target for cervical cancer. |
| first_indexed | 2024-12-11T05:49:40Z |
| format | Article |
| id | doaj.art-5d4af03a49754f9bb9e20b7e46b7ad7f |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-11T05:49:40Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-5d4af03a49754f9bb9e20b7e46b7ad7f2022-12-22T01:18:51ZengBMCWorld Journal of Surgical Oncology1477-78192022-04-012011810.1186/s12957-022-02585-3KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathwayJie Zhang0Gulimire Buranjiang1Zuohelaguli Mutalifu2Hua Jin3Liyan Yao4Department of Obstetrics and Gynecology, Second Affiliated Hospital of Xinjiang Medical UniversityDepartment of Obstetrics and Gynecology, Second Affiliated Hospital of Xinjiang Medical UniversityDepartment of Obstetrics and Gynecology, Second Affiliated Hospital of Xinjiang Medical UniversityDepartment of Obstetrics and Gynecology, Second Affiliated Hospital of Xinjiang Medical UniversityDepartment of Obstetrics and Gynecology, Second Affiliated Hospital of Xinjiang Medical UniversityAbstract Background Cervical cancer is a kind of malignant gynecological tumor. The first choice for treating cervical cancer is still a combination of surgery and chemoradiotherapy, but the 5-year survival rate remains poor. Therefore, researchers are trying to find new ways to diagnose and treat cervical cancer early. Methods The expression level of KIF14 in cells and tissues was determined via qRT–PCR. The ability of the cells to proliferate, migrate, and invade was examined using CCK-8 assay kits, colony formation assays, and Transwell chambers. The expression levels of Cyclin D1, Cyclin B1, p21, and p27 were also detected using western blot assays. Results The results suggested that p27 is a key regulatory factor in the KIF14-mediated regulation of the cell cycle. In addition, KIF14 knockdown promotes malignancy in cervical cancer cells by inhibiting p27 degradation, resulting in cell cycle arrest. Conclusions KIF14 is an oncogene in cervical cancer, and knocking down KIF14 causes cell cycle arrest by inhibiting p27 degradation, thus affecting cell viability, proliferation, and migration. These results provide a potential therapeutic target for cervical cancer.https://doi.org/10.1186/s12957-022-02585-3Cervical cancerKIF14, Cell cycle arrestp27Degradation |
| spellingShingle | Jie Zhang Gulimire Buranjiang Zuohelaguli Mutalifu Hua Jin Liyan Yao KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway World Journal of Surgical Oncology Cervical cancer KIF14, Cell cycle arrest p27 Degradation |
| title | KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway |
| title_full | KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway |
| title_fullStr | KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway |
| title_full_unstemmed | KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway |
| title_short | KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway |
| title_sort | kif14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27kip1 pathway |
| topic | Cervical cancer KIF14, Cell cycle arrest p27 Degradation |
| url | https://doi.org/10.1186/s12957-022-02585-3 |
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