Influence of Nitrogen Fertilizer on the Antioxidative Potential of Basil Varieties (<i>Ocimum basilicum</i> L.)
Total phenolic content (TPC) in extracts of basil depended on the cultivar and type of fertilization used in cultivation. TPC was determined spectrophotometrically with the Folin–Ciocalteu reagent. The antioxidant activity of extracts was analyzed by scavenging of DPPH and ABTS radicals, on the basi...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| author | Marzanna Hęś Anna Golcz Anna Gramza-Michałowska Anna Jędrusek-Golińska Krzysztof Dziedzic Sylwia Mildner-Szkudlarz |
| author_facet | Marzanna Hęś Anna Golcz Anna Gramza-Michałowska Anna Jędrusek-Golińska Krzysztof Dziedzic Sylwia Mildner-Szkudlarz |
| author_sort | Marzanna Hęś |
| collection | DOAJ |
| description | Total phenolic content (TPC) in extracts of basil depended on the cultivar and type of fertilization used in cultivation. TPC was determined spectrophotometrically with the Folin–Ciocalteu reagent. The antioxidant activity of extracts was analyzed by scavenging of DPPH and ABTS radicals, on the basis of metal chelating ability (MetChel) and ferric reducing antioxidant power (FRAP). The greatest TPC was determined in the purple cultivars—141.35 and 165.44 mg gallic acid/g d.m. for fertilized with ammonium nitrate (NH<sub>4</sub>NO<sub>3</sub>) and ammonium sulfate ((NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>), respectively. Their extracts had the greatest antioxidant capacity in the majority of the methods used. The results varied depending on the modelling system used. The amount of polyphenols in individual basil cultivars differed significantly (<i>p</i> < 0.05) depending on the fertilization used in the culture. Regarding TPC, DPPH, ABTS, FRAP, and MetChel variables, we observed a significant effect for the applied cultivation. In the case of MetChel factor, lower results of all investigated basil species were observed for cultivation with ammonium sulfate. PCA demonstrated in the present study shows that Sweet and Cinnamon Basil samples cultivated with ammonium nitrate create a separated group. We recommend cultivation with ammonium sulfate fertilizers for these varieties of basil. The high content of phenolic compounds demonstrated in Sweet and Cinnamon Basil cultivated with ammonium sulfate, and thus associated antioxidant activity, indicates that it can constitute a valuable source for bioactive compounds in a balanced diet. |
| first_indexed | 2024-03-10T01:28:41Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:28:41Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5d4ee83f573e4c38bcf7d30942cf66a62023-11-23T13:45:30ZengMDPI AGMolecules1420-30492022-09-012717563610.3390/molecules27175636Influence of Nitrogen Fertilizer on the Antioxidative Potential of Basil Varieties (<i>Ocimum basilicum</i> L.)Marzanna Hęś0Anna Golcz1Anna Gramza-Michałowska2Anna Jędrusek-Golińska3Krzysztof Dziedzic4Sylwia Mildner-Szkudlarz5Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandFaculty of Horticulture and Landscape Architecture, Poznań University of Life Sciences, Zgorzelecka 4, 60-198 Poznań, PolandFaculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandFaculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandFaculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandFaculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandTotal phenolic content (TPC) in extracts of basil depended on the cultivar and type of fertilization used in cultivation. TPC was determined spectrophotometrically with the Folin–Ciocalteu reagent. The antioxidant activity of extracts was analyzed by scavenging of DPPH and ABTS radicals, on the basis of metal chelating ability (MetChel) and ferric reducing antioxidant power (FRAP). The greatest TPC was determined in the purple cultivars—141.35 and 165.44 mg gallic acid/g d.m. for fertilized with ammonium nitrate (NH<sub>4</sub>NO<sub>3</sub>) and ammonium sulfate ((NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>), respectively. Their extracts had the greatest antioxidant capacity in the majority of the methods used. The results varied depending on the modelling system used. The amount of polyphenols in individual basil cultivars differed significantly (<i>p</i> < 0.05) depending on the fertilization used in the culture. Regarding TPC, DPPH, ABTS, FRAP, and MetChel variables, we observed a significant effect for the applied cultivation. In the case of MetChel factor, lower results of all investigated basil species were observed for cultivation with ammonium sulfate. PCA demonstrated in the present study shows that Sweet and Cinnamon Basil samples cultivated with ammonium nitrate create a separated group. We recommend cultivation with ammonium sulfate fertilizers for these varieties of basil. The high content of phenolic compounds demonstrated in Sweet and Cinnamon Basil cultivated with ammonium sulfate, and thus associated antioxidant activity, indicates that it can constitute a valuable source for bioactive compounds in a balanced diet.https://www.mdpi.com/1420-3049/27/17/5636basil cultivarsnitrogen fertilizationantioxidant activity |
| spellingShingle | Marzanna Hęś Anna Golcz Anna Gramza-Michałowska Anna Jędrusek-Golińska Krzysztof Dziedzic Sylwia Mildner-Szkudlarz Influence of Nitrogen Fertilizer on the Antioxidative Potential of Basil Varieties (<i>Ocimum basilicum</i> L.) Molecules basil cultivars nitrogen fertilization antioxidant activity |
| title | Influence of Nitrogen Fertilizer on the Antioxidative Potential of Basil Varieties (<i>Ocimum basilicum</i> L.) |
| title_full | Influence of Nitrogen Fertilizer on the Antioxidative Potential of Basil Varieties (<i>Ocimum basilicum</i> L.) |
| title_fullStr | Influence of Nitrogen Fertilizer on the Antioxidative Potential of Basil Varieties (<i>Ocimum basilicum</i> L.) |
| title_full_unstemmed | Influence of Nitrogen Fertilizer on the Antioxidative Potential of Basil Varieties (<i>Ocimum basilicum</i> L.) |
| title_short | Influence of Nitrogen Fertilizer on the Antioxidative Potential of Basil Varieties (<i>Ocimum basilicum</i> L.) |
| title_sort | influence of nitrogen fertilizer on the antioxidative potential of basil varieties i ocimum basilicum i l |
| topic | basil cultivars nitrogen fertilization antioxidant activity |
| url | https://www.mdpi.com/1420-3049/27/17/5636 |
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