A Novel Polysaccharide from <i>Sargassum weizhouense</i>: Extraction Optimization, Structural Characterization, Antiviral and Antioxidant Effects
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important pathogens in the global swine industry over the past three decades. There is no licensed antiviral medication that can effectively control this infection. In the present study, the structure of SP-1...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/10/1832 |
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| author | Yi Zhao Jiaji Chen Yiqu Ding Mengyuan Luo Yanmei Tong Tingjun Hu Yingyi Wei |
| author_facet | Yi Zhao Jiaji Chen Yiqu Ding Mengyuan Luo Yanmei Tong Tingjun Hu Yingyi Wei |
| author_sort | Yi Zhao |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important pathogens in the global swine industry over the past three decades. There is no licensed antiviral medication that can effectively control this infection. In the present study, the structure of SP-1 isolated and purified from <i>Sargassum weizhouense</i> was analyzed, and its antioxidant capacity and antiviral effect in MARC-145 cells against PRRSV were investigated. The results showed that SP-1 is a novel polysaccharide which mainly is composed of →4)-β-D-ManpA-(1→, →4)-α-L-GulpA-(1→ and a small amount of →4)-β-D-GalpA-(1→. PRRSV adsorption, replication, and release were all suppressed by SP-1. SP-1 therapy down-regulated mRNA expression of the CD163 receptor while increasing the antioxidant gene expression of Nrf2, TXNIP, and HO-1; increasing the protein expression of NQO1 and HO-1; and drastically reducing the protein expression of p-p65. The findings indicated that SP-1 reduces PRRSV adsorption, replication, and release through blocking the expression of the crucial CD163 receptor during infection. Meanwhile, SP-1 exerts antioxidant effects in PRRSV-infected cells through the activation of the Nrf2-HO1 signaling pathway. |
| first_indexed | 2024-03-10T21:29:21Z |
| format | Article |
| id | doaj.art-5d4f687d839e45e89786e795e8d46fc5 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T21:29:21Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5d4f687d839e45e89786e795e8d46fc52023-11-19T15:27:49ZengMDPI AGAntioxidants2076-39212023-10-011210183210.3390/antiox12101832A Novel Polysaccharide from <i>Sargassum weizhouense</i>: Extraction Optimization, Structural Characterization, Antiviral and Antioxidant EffectsYi Zhao0Jiaji Chen1Yiqu Ding2Mengyuan Luo3Yanmei Tong4Tingjun Hu5Yingyi Wei6Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaPorcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important pathogens in the global swine industry over the past three decades. There is no licensed antiviral medication that can effectively control this infection. In the present study, the structure of SP-1 isolated and purified from <i>Sargassum weizhouense</i> was analyzed, and its antioxidant capacity and antiviral effect in MARC-145 cells against PRRSV were investigated. The results showed that SP-1 is a novel polysaccharide which mainly is composed of →4)-β-D-ManpA-(1→, →4)-α-L-GulpA-(1→ and a small amount of →4)-β-D-GalpA-(1→. PRRSV adsorption, replication, and release were all suppressed by SP-1. SP-1 therapy down-regulated mRNA expression of the CD163 receptor while increasing the antioxidant gene expression of Nrf2, TXNIP, and HO-1; increasing the protein expression of NQO1 and HO-1; and drastically reducing the protein expression of p-p65. The findings indicated that SP-1 reduces PRRSV adsorption, replication, and release through blocking the expression of the crucial CD163 receptor during infection. Meanwhile, SP-1 exerts antioxidant effects in PRRSV-infected cells through the activation of the Nrf2-HO1 signaling pathway.https://www.mdpi.com/2076-3921/12/10/1832<i>Sargassum</i> polysaccharide (SP)PRRSVantioxidant capacityantiviral effect |
| spellingShingle | Yi Zhao Jiaji Chen Yiqu Ding Mengyuan Luo Yanmei Tong Tingjun Hu Yingyi Wei A Novel Polysaccharide from <i>Sargassum weizhouense</i>: Extraction Optimization, Structural Characterization, Antiviral and Antioxidant Effects Antioxidants <i>Sargassum</i> polysaccharide (SP) PRRSV antioxidant capacity antiviral effect |
| title | A Novel Polysaccharide from <i>Sargassum weizhouense</i>: Extraction Optimization, Structural Characterization, Antiviral and Antioxidant Effects |
| title_full | A Novel Polysaccharide from <i>Sargassum weizhouense</i>: Extraction Optimization, Structural Characterization, Antiviral and Antioxidant Effects |
| title_fullStr | A Novel Polysaccharide from <i>Sargassum weizhouense</i>: Extraction Optimization, Structural Characterization, Antiviral and Antioxidant Effects |
| title_full_unstemmed | A Novel Polysaccharide from <i>Sargassum weizhouense</i>: Extraction Optimization, Structural Characterization, Antiviral and Antioxidant Effects |
| title_short | A Novel Polysaccharide from <i>Sargassum weizhouense</i>: Extraction Optimization, Structural Characterization, Antiviral and Antioxidant Effects |
| title_sort | novel polysaccharide from i sargassum weizhouense i extraction optimization structural characterization antiviral and antioxidant effects |
| topic | <i>Sargassum</i> polysaccharide (SP) PRRSV antioxidant capacity antiviral effect |
| url | https://www.mdpi.com/2076-3921/12/10/1832 |
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