The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6
The stationary-phase sigma factor, RpoS, influences the expression of factors important in survival of Pseudomonas chlororaphis O6 in the rhizosphere. A partial proteomic profile of a rpoS mutant in P. chlororaphis O6 was conducted to identify proteins under RpoS regulation. Five of 14 differentiall...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2013-09-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174801/ |
| _version_ | 1818982739804684288 |
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| author | Sang A Oh Ji Soo Kim Ju Yeon Park Song Hee Han Christian Dimkpa Anne J. Anderson Young Cheol Kim |
| author_facet | Sang A Oh Ji Soo Kim Ju Yeon Park Song Hee Han Christian Dimkpa Anne J. Anderson Young Cheol Kim |
| author_sort | Sang A Oh |
| collection | DOAJ |
| description | The stationary-phase sigma factor, RpoS, influences the expression of factors important in survival of Pseudomonas chlororaphis O6 in the rhizosphere. A partial proteomic profile of a rpoS mutant in P. chlororaphis O6 was conducted to identify proteins under RpoS regulation. Five of 14 differentially regulated proteins had unknown roles. Changes in levels of proteins in P. chlororaphis O6 rpoS mutant were associated with iron metabolism, and protection against oxidative stress. The P. chlororaphis O6 rpoS mutant showed increased production of a pyoverdine-like siderophore, indole acetic acid, and altered isozyme patterns for peroxidase, catalase and superoxide dismutase. Consequently, sensitivity to hydrogen peroxide exposure increased in the P. chlororaphis O6 rpoS mutant, compared with the wild type. Taken together, RpoS exerted regulatory control over factors important for the habitat of P. chlororaphis O6 in soil and on root surfaces. The properties of several of the proteins in the RpoS regulon are currently unknown. |
| first_indexed | 2024-12-20T17:52:00Z |
| format | Article |
| id | doaj.art-5d57e8fbd11a4c8d8e6688cfff4d1a9d |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-20T17:52:00Z |
| publishDate | 2013-09-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-5d57e8fbd11a4c8d8e6688cfff4d1a9d2022-12-21T19:30:50ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542013-09-0129332332910.5423/PPJ.NT.01.2013.0013PPJ.NT.01.2013.001The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6Sang A Oh0Ji Soo Kim1Ju Yeon Park2Song Hee Han3Christian Dimkpa4Anne J. Anderson5Young Cheol Kim6Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju 500-757, KoreaInstitute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju 500-757, KoreaInstitute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju 500-757, KoreaInstitute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju 500-757, KoreaDepartment of Biology, Utah State University, Logan, UT, USADepartment of Biology, Utah State University, Logan, UT, USAInstitute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju 500-757, KoreaThe stationary-phase sigma factor, RpoS, influences the expression of factors important in survival of Pseudomonas chlororaphis O6 in the rhizosphere. A partial proteomic profile of a rpoS mutant in P. chlororaphis O6 was conducted to identify proteins under RpoS regulation. Five of 14 differentially regulated proteins had unknown roles. Changes in levels of proteins in P. chlororaphis O6 rpoS mutant were associated with iron metabolism, and protection against oxidative stress. The P. chlororaphis O6 rpoS mutant showed increased production of a pyoverdine-like siderophore, indole acetic acid, and altered isozyme patterns for peroxidase, catalase and superoxide dismutase. Consequently, sensitivity to hydrogen peroxide exposure increased in the P. chlororaphis O6 rpoS mutant, compared with the wild type. Taken together, RpoS exerted regulatory control over factors important for the habitat of P. chlororaphis O6 in soil and on root surfaces. The properties of several of the proteins in the RpoS regulon are currently unknown.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174801/indole acetic acidironoxidative stressproteomic analysissigma factor RpoS |
| spellingShingle | Sang A Oh Ji Soo Kim Ju Yeon Park Song Hee Han Christian Dimkpa Anne J. Anderson Young Cheol Kim The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6 The Plant Pathology Journal indole acetic acid iron oxidative stress proteomic analysis sigma factor RpoS |
| title | The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6 |
| title_full | The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6 |
| title_fullStr | The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6 |
| title_full_unstemmed | The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6 |
| title_short | The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6 |
| title_sort | rpos sigma factor negatively regulates production of iaa and siderophore in a biocontrol rhizobacterium pseudomonas chlororaphis o6 |
| topic | indole acetic acid iron oxidative stress proteomic analysis sigma factor RpoS |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174801/ |
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