Inhibitory effect of endostar combined with radiotherapy on gastric cancer animal models
Abstract Background Inhibitory effect of endostar combined with radiotherapy on gastric cancer (GC) animal models and its effect on transforming growth factor-β1 (TGF-β1) and inter-leukin-10 (IL-10) were evaluated. Methods Forty mice of GC model xenograft tumors were prepared and randomly divided in...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12957-020-01937-1 |
| _version_ | 1811299145371090944 |
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| author | Qitian Chen Ran Chen Youhong Dong |
| author_facet | Qitian Chen Ran Chen Youhong Dong |
| author_sort | Qitian Chen |
| collection | DOAJ |
| description | Abstract Background Inhibitory effect of endostar combined with radiotherapy on gastric cancer (GC) animal models and its effect on transforming growth factor-β1 (TGF-β1) and inter-leukin-10 (IL-10) were evaluated. Methods Forty mice of GC model xenograft tumors were prepared and randomly divided into blank control group, endostar group, radiotherapy group, and endostar combined with radiotherapy group (combination group). From the 14th day, a vernier caliper was used for measuring the long and short diameters of the xenograft tumors. The formula V = ab2/2 was used for calculating the tumor volume and to obtain its average value. Tumor growth curves were plotted to calculate the tumor inhibition rate. The growth of xenograft tumors and the behavioral changes of mice were observed. Enzyme-linked immunosorbent assay (ELISA) was used for detecting the expression levels of IL-10 and TGF-β1. Results The tumor growth in the combination group was significantly inhibited, and the tumor volume was the smallest compared with the other groups (p < 0.05). Compared to the blank control group, the tumor inhibition rate was 11.8% in endostar group, 33.0% in radiotherapy group, and 52.1% in combination group (p < 0.01). Endostar combined with radiotherapy had an interaction in decreasing the expression levels of TGF-β1 and IL-10 (F = 4.35 and 5.12, p < 0.05). Leucocyte count was significantly higher in control and combination groups than that in endostar and radiotherapy groups. The body weight of mice in endostar and radiotherapy groups decreased after treatment (p < 0.05). The body weight of mice after treatment in control and combination groups increased, with a statistically significant difference compared to that before treatment (p < 0.05). There was a statistically significant difference among all groups after treatment (F = 198.1, p < 0.01). Conclusions Endostar combined with radiotherapy can inhibit tumor growth and downregulate the expression levels of TGF-β1 and IL-10 through synergistic action. |
| first_indexed | 2024-04-13T06:31:55Z |
| format | Article |
| id | doaj.art-5d5cffa4b9b743d9a2e9d62f6429354d |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-04-13T06:31:55Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-5d5cffa4b9b743d9a2e9d62f6429354d2022-12-22T02:58:07ZengBMCWorld Journal of Surgical Oncology1477-78192020-07-011811710.1186/s12957-020-01937-1Inhibitory effect of endostar combined with radiotherapy on gastric cancer animal modelsQitian Chen0Ran Chen1Youhong Dong2Department of Oncology, Xiangyang No. 1 People’s Hospital, Hubei University of MedicineDepartment of Oncology, Xiangyang No. 1 People’s Hospital, Hubei University of MedicineDepartment of Oncology, Xiangyang No. 1 People’s Hospital, Hubei University of MedicineAbstract Background Inhibitory effect of endostar combined with radiotherapy on gastric cancer (GC) animal models and its effect on transforming growth factor-β1 (TGF-β1) and inter-leukin-10 (IL-10) were evaluated. Methods Forty mice of GC model xenograft tumors were prepared and randomly divided into blank control group, endostar group, radiotherapy group, and endostar combined with radiotherapy group (combination group). From the 14th day, a vernier caliper was used for measuring the long and short diameters of the xenograft tumors. The formula V = ab2/2 was used for calculating the tumor volume and to obtain its average value. Tumor growth curves were plotted to calculate the tumor inhibition rate. The growth of xenograft tumors and the behavioral changes of mice were observed. Enzyme-linked immunosorbent assay (ELISA) was used for detecting the expression levels of IL-10 and TGF-β1. Results The tumor growth in the combination group was significantly inhibited, and the tumor volume was the smallest compared with the other groups (p < 0.05). Compared to the blank control group, the tumor inhibition rate was 11.8% in endostar group, 33.0% in radiotherapy group, and 52.1% in combination group (p < 0.01). Endostar combined with radiotherapy had an interaction in decreasing the expression levels of TGF-β1 and IL-10 (F = 4.35 and 5.12, p < 0.05). Leucocyte count was significantly higher in control and combination groups than that in endostar and radiotherapy groups. The body weight of mice in endostar and radiotherapy groups decreased after treatment (p < 0.05). The body weight of mice after treatment in control and combination groups increased, with a statistically significant difference compared to that before treatment (p < 0.05). There was a statistically significant difference among all groups after treatment (F = 198.1, p < 0.01). Conclusions Endostar combined with radiotherapy can inhibit tumor growth and downregulate the expression levels of TGF-β1 and IL-10 through synergistic action.http://link.springer.com/article/10.1186/s12957-020-01937-1EndostarGastric cancer animal modelInterleukin-10RadiotherapyTransforming growth factor-β1 |
| spellingShingle | Qitian Chen Ran Chen Youhong Dong Inhibitory effect of endostar combined with radiotherapy on gastric cancer animal models World Journal of Surgical Oncology Endostar Gastric cancer animal model Interleukin-10 Radiotherapy Transforming growth factor-β1 |
| title | Inhibitory effect of endostar combined with radiotherapy on gastric cancer animal models |
| title_full | Inhibitory effect of endostar combined with radiotherapy on gastric cancer animal models |
| title_fullStr | Inhibitory effect of endostar combined with radiotherapy on gastric cancer animal models |
| title_full_unstemmed | Inhibitory effect of endostar combined with radiotherapy on gastric cancer animal models |
| title_short | Inhibitory effect of endostar combined with radiotherapy on gastric cancer animal models |
| title_sort | inhibitory effect of endostar combined with radiotherapy on gastric cancer animal models |
| topic | Endostar Gastric cancer animal model Interleukin-10 Radiotherapy Transforming growth factor-β1 |
| url | http://link.springer.com/article/10.1186/s12957-020-01937-1 |
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