Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes
In this paper we report an X-ray diffraction study on the phase behavior of binary lipid mixtures of 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (DHA-PE) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at low concentrations below 5.0 mol% DHA-PE. Our results show that DHA-P...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Membranes |
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| Online Access: | http://www.mdpi.com/2077-0375/5/4/857 |
| _version_ | 1797712396425363456 |
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| author | Chai Lor Linda S. Hirst |
| author_facet | Chai Lor Linda S. Hirst |
| author_sort | Chai Lor |
| collection | DOAJ |
| description | In this paper we report an X-ray diffraction study on the phase behavior of binary lipid mixtures of 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (DHA-PE) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at low concentrations below 5.0 mol% DHA-PE. Our results show that DHA-PE induces phase separation into a DHA rich liquid crystalline (Lα) phase and a DHA poor gel (Lβ′) phase at overall DHA-PE concentrations as low as 0.1 mol%. In addition, we find that the structure of the Lβ′ phase, from which the DHA-PE molecules are largely excluded, is modified in the phase-separated state at low DHA-PE concentrations, with a decrease in bilayer thickness of 1.34 nm for 0.1 mol% at room temperature, compared to pure DPPC bilayers. This result is contrary to that seen in similar studies on mono-unsaturated lipids where an increase in bilayer thickness is observed. The surprising effect of such low DHA-PE concentrations on membrane structure may be important in understanding the role of highly polyunsaturated lipids in biological membrane-based structures and similar artificial surfactant systems. |
| first_indexed | 2024-03-12T07:20:12Z |
| format | Article |
| id | doaj.art-5d62bb0820c94ec08166652e6d41b21a |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-12T07:20:12Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-5d62bb0820c94ec08166652e6d41b21a2023-09-02T22:27:37ZengMDPI AGMembranes2077-03752015-12-015485787410.3390/membranes5040857membranes5040857Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid MembranesChai Lor0Linda S. Hirst1Bioengineering and Small Scale Technologies, School of Engineering, University of California, Merced, CA 95343, USADepartment of Physics, School of Natural Sciences, University of California, Merced, CA 95343, USAIn this paper we report an X-ray diffraction study on the phase behavior of binary lipid mixtures of 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (DHA-PE) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at low concentrations below 5.0 mol% DHA-PE. Our results show that DHA-PE induces phase separation into a DHA rich liquid crystalline (Lα) phase and a DHA poor gel (Lβ′) phase at overall DHA-PE concentrations as low as 0.1 mol%. In addition, we find that the structure of the Lβ′ phase, from which the DHA-PE molecules are largely excluded, is modified in the phase-separated state at low DHA-PE concentrations, with a decrease in bilayer thickness of 1.34 nm for 0.1 mol% at room temperature, compared to pure DPPC bilayers. This result is contrary to that seen in similar studies on mono-unsaturated lipids where an increase in bilayer thickness is observed. The surprising effect of such low DHA-PE concentrations on membrane structure may be important in understanding the role of highly polyunsaturated lipids in biological membrane-based structures and similar artificial surfactant systems.http://www.mdpi.com/2077-0375/5/4/857DHAlipid bilayergel phaseX-ray diffraction |
| spellingShingle | Chai Lor Linda S. Hirst Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes Membranes DHA lipid bilayer gel phase X-ray diffraction |
| title | Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes |
| title_full | Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes |
| title_fullStr | Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes |
| title_full_unstemmed | Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes |
| title_short | Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes |
| title_sort | effects of low concentrations of docosahexaenoic acid on the structure and phase behavior of model lipid membranes |
| topic | DHA lipid bilayer gel phase X-ray diffraction |
| url | http://www.mdpi.com/2077-0375/5/4/857 |
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