An Analytical Method for Simultaneous Measurement of Various Cyanotoxins Using Stable Isotope-Labeled Surrogates and a Microbial Flora Analysis to Assign Each Cyanotoxin to its Source
Cyanotoxins produced by blue-green algae in lakes are among the most serious threats to water quality worldwide. As global warming rapidly extends the locations and timing of blue-green algae blooms, a simple and accessible method for the detection and quantification of cy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Japan Society on Water Environment
2022-01-01
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| Series: | Journal of Water and Environment Technology |
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| Online Access: |
https://www.jstage.jst.go.jp/article/jwet/20/6/20_22-005/_pdf
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| _version_ | 1797894395101446144 |
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| author | Masaya Matsuki Nobuhiro Shimizu Kazuhiro Tobiishi Yoshito Tanaka Haruyo Yamaguchi Tomoharu Sano |
| author_facet | Masaya Matsuki Nobuhiro Shimizu Kazuhiro Tobiishi Yoshito Tanaka Haruyo Yamaguchi Tomoharu Sano |
| author_sort | Masaya Matsuki |
| collection | DOAJ |
| description | Cyanotoxins produced by blue-green algae in lakes are among the most serious threats to water quality worldwide. As global warming rapidly extends the locations and timing of blue-green algae blooms, a simple and accessible method for the detection and quantification of cyanotoxins in fresh water is increasingly necessary. Here, a quick, simple and accessible simultaneous analytical method for five cyanotoxins (cylindrospermopsin, anatoxin-a, microcystin-RR, YR and LR) is reported. This method has three advantages. First, it does not require complicated operations, such as a concentration operation. Second, it employs an HPLC column without high pressure. Third, the use of stable isotope-labeled surrogates enables correct identification and precise quantification of cyanotoxins. The method was applied to the lakes of Fukuoka Prefecture in Japan, and four of the five above-named cyanotoxins (i.e., all but cylindrospermopsin) were detected. The limits of quantification were 20–43 ng/L, which were considerably lower than the WHO guideline values. The recovery levels were 97–104%. Microbial flora analysis revealed that the sources of anatoxin-a were Pseudanabaena limnetica and Cuspidothrix issatschenkoi, and the source of microcystins was the group A1 of Microcystis aeruginosa. This study provides a quick, easy and accessible method for the worldwide monitoring of cyanotoxin levels. |
| first_indexed | 2024-04-10T07:08:21Z |
| format | Article |
| id | doaj.art-5d650fb05ce4458f96d1a1ca4f656fd9 |
| institution | Directory Open Access Journal |
| issn | 1348-2165 |
| language | English |
| last_indexed | 2024-04-10T07:08:21Z |
| publishDate | 2022-01-01 |
| publisher | Japan Society on Water Environment |
| record_format | Article |
| series | Journal of Water and Environment Technology |
| spelling | doaj.art-5d650fb05ce4458f96d1a1ca4f656fd92023-02-27T01:41:21ZengJapan Society on Water EnvironmentJournal of Water and Environment Technology1348-21652022-01-0120626127210.2965/jwet.22-00510.2965/jwet.22-005An Analytical Method for Simultaneous Measurement of Various Cyanotoxins Using Stable Isotope-Labeled Surrogates and a Microbial Flora Analysis to Assign Each Cyanotoxin to its SourceMasaya Matsuki0Nobuhiro Shimizu1Kazuhiro Tobiishi2Yoshito Tanaka3Haruyo Yamaguchi4Tomoharu Sano5 Fukuoka Institute of Health and Environmental Sciences, Dazaifu, Japan Fukuoka Institute of Health and Environmental Sciences, Dazaifu, Japan Fukuoka Institute of Health and Environmental Sciences, Dazaifu, Japan Fukuoka Institute of Health and Environmental Sciences, Dazaifu, Japan Biodiversity Division, National Institute for Environmental Studies, Tsukuba, Japan Health and Environmental Risk Division, National Institute for Environmental Studies, Tsukuba, Japan Cyanotoxins produced by blue-green algae in lakes are among the most serious threats to water quality worldwide. As global warming rapidly extends the locations and timing of blue-green algae blooms, a simple and accessible method for the detection and quantification of cyanotoxins in fresh water is increasingly necessary. Here, a quick, simple and accessible simultaneous analytical method for five cyanotoxins (cylindrospermopsin, anatoxin-a, microcystin-RR, YR and LR) is reported. This method has three advantages. First, it does not require complicated operations, such as a concentration operation. Second, it employs an HPLC column without high pressure. Third, the use of stable isotope-labeled surrogates enables correct identification and precise quantification of cyanotoxins. The method was applied to the lakes of Fukuoka Prefecture in Japan, and four of the five above-named cyanotoxins (i.e., all but cylindrospermopsin) were detected. The limits of quantification were 20–43 ng/L, which were considerably lower than the WHO guideline values. The recovery levels were 97–104%. Microbial flora analysis revealed that the sources of anatoxin-a were Pseudanabaena limnetica and Cuspidothrix issatschenkoi, and the source of microcystins was the group A1 of Microcystis aeruginosa. This study provides a quick, easy and accessible method for the worldwide monitoring of cyanotoxin levels. https://www.jstage.jst.go.jp/article/jwet/20/6/20_22-005/_pdf cyanotoxinsmicrocystinanatoxin-acylindrospermopsinmicrobial flora |
| spellingShingle | Masaya Matsuki Nobuhiro Shimizu Kazuhiro Tobiishi Yoshito Tanaka Haruyo Yamaguchi Tomoharu Sano An Analytical Method for Simultaneous Measurement of Various Cyanotoxins Using Stable Isotope-Labeled Surrogates and a Microbial Flora Analysis to Assign Each Cyanotoxin to its Source Journal of Water and Environment Technology cyanotoxins microcystin anatoxin-a cylindrospermopsin microbial flora |
| title | An Analytical Method for Simultaneous Measurement of Various Cyanotoxins Using Stable Isotope-Labeled Surrogates and a Microbial Flora Analysis to Assign Each Cyanotoxin to its Source |
| title_full | An Analytical Method for Simultaneous Measurement of Various Cyanotoxins Using Stable Isotope-Labeled Surrogates and a Microbial Flora Analysis to Assign Each Cyanotoxin to its Source |
| title_fullStr | An Analytical Method for Simultaneous Measurement of Various Cyanotoxins Using Stable Isotope-Labeled Surrogates and a Microbial Flora Analysis to Assign Each Cyanotoxin to its Source |
| title_full_unstemmed | An Analytical Method for Simultaneous Measurement of Various Cyanotoxins Using Stable Isotope-Labeled Surrogates and a Microbial Flora Analysis to Assign Each Cyanotoxin to its Source |
| title_short | An Analytical Method for Simultaneous Measurement of Various Cyanotoxins Using Stable Isotope-Labeled Surrogates and a Microbial Flora Analysis to Assign Each Cyanotoxin to its Source |
| title_sort | analytical method for simultaneous measurement of various cyanotoxins using stable isotope labeled surrogates and a microbial flora analysis to assign each cyanotoxin to its source |
| topic | cyanotoxins microcystin anatoxin-a cylindrospermopsin microbial flora |
| url |
https://www.jstage.jst.go.jp/article/jwet/20/6/20_22-005/_pdf
|
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