Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated <i>Salmonella</i> Enteritidis Vaccine Candidate in Chickens
An ideal vaccine for controlling <i>Salmonella</i> infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against <i>Salmonella</i> Enteritidis (<i>S</i>. Enteritidis) have been used as a potential control...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/9/1405 |
| _version_ | 1797481623532339200 |
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| author | Hyunjin Shin Tae-Min La Hong-Jae Lee Taesoo Kim Seung-un Song Eunjin Park Gyu-Hyung Park In-Soo Choi Seung-Yong Park Joong-Bok Lee Sang-Won Lee |
| author_facet | Hyunjin Shin Tae-Min La Hong-Jae Lee Taesoo Kim Seung-un Song Eunjin Park Gyu-Hyung Park In-Soo Choi Seung-Yong Park Joong-Bok Lee Sang-Won Lee |
| author_sort | Hyunjin Shin |
| collection | DOAJ |
| description | An ideal vaccine for controlling <i>Salmonella</i> infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against <i>Salmonella</i> Enteritidis (<i>S</i>. Enteritidis) have been used as a potential control method of <i>Salmonella</i> infection in the poultry industry. However, live attenuated vaccines can persistently infect poultry for long periods and can become virulent revertant strains. In this study, we assessed the immune responses and protective efficacy of a temperature-sensitive attenuated <i>S.</i> Enteritidis mutant as a potential vaccine candidate. In addition, we evaluated the combined vaccine administration methods to maximize both humoral and cellular immune responses in chickens induced by the vaccine candidate. Immune responses and protective efficacy were compared between the Oral/IM group, vaccinated using one oral dose at four weeks old and a booster intramuscular dose at seven weeks old, and the IM/Oral group, vaccinated using one intramuscular dose at four weeks old and a booster oral dose at seven weeks old. The Oral/IM group showed stronger immune responses than those of the IM/Oral group. Spleens from the Oral/IM group showed a promising tendency of reduction of challenged <i>Salmonella</i> compared with those of other groups. Overall, the results indicated that the <i>S</i>. Enteritidis mutant strain is a promising live attenuated vaccine candidate with good efficacy. |
| first_indexed | 2024-03-09T22:17:14Z |
| format | Article |
| id | doaj.art-5d68bf07bb8a4f98967f0c8b0ebdd0cf |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T22:17:14Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-5d68bf07bb8a4f98967f0c8b0ebdd0cf2023-11-23T19:20:31ZengMDPI AGVaccines2076-393X2022-08-01109140510.3390/vaccines10091405Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated <i>Salmonella</i> Enteritidis Vaccine Candidate in ChickensHyunjin Shin0Tae-Min La1Hong-Jae Lee2Taesoo Kim3Seung-un Song4Eunjin Park5Gyu-Hyung Park6In-Soo Choi7Seung-Yong Park8Joong-Bok Lee9Sang-Won Lee10College of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCollege of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaAn ideal vaccine for controlling <i>Salmonella</i> infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against <i>Salmonella</i> Enteritidis (<i>S</i>. Enteritidis) have been used as a potential control method of <i>Salmonella</i> infection in the poultry industry. However, live attenuated vaccines can persistently infect poultry for long periods and can become virulent revertant strains. In this study, we assessed the immune responses and protective efficacy of a temperature-sensitive attenuated <i>S.</i> Enteritidis mutant as a potential vaccine candidate. In addition, we evaluated the combined vaccine administration methods to maximize both humoral and cellular immune responses in chickens induced by the vaccine candidate. Immune responses and protective efficacy were compared between the Oral/IM group, vaccinated using one oral dose at four weeks old and a booster intramuscular dose at seven weeks old, and the IM/Oral group, vaccinated using one intramuscular dose at four weeks old and a booster oral dose at seven weeks old. The Oral/IM group showed stronger immune responses than those of the IM/Oral group. Spleens from the Oral/IM group showed a promising tendency of reduction of challenged <i>Salmonella</i> compared with those of other groups. Overall, the results indicated that the <i>S</i>. Enteritidis mutant strain is a promising live attenuated vaccine candidate with good efficacy.https://www.mdpi.com/2076-393X/10/9/1405<i>Salmonella</i> Enteritidislive attenuated vaccineimmune responseprotective efficacy |
| spellingShingle | Hyunjin Shin Tae-Min La Hong-Jae Lee Taesoo Kim Seung-un Song Eunjin Park Gyu-Hyung Park In-Soo Choi Seung-Yong Park Joong-Bok Lee Sang-Won Lee Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated <i>Salmonella</i> Enteritidis Vaccine Candidate in Chickens Vaccines <i>Salmonella</i> Enteritidis live attenuated vaccine immune response protective efficacy |
| title | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated <i>Salmonella</i> Enteritidis Vaccine Candidate in Chickens |
| title_full | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated <i>Salmonella</i> Enteritidis Vaccine Candidate in Chickens |
| title_fullStr | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated <i>Salmonella</i> Enteritidis Vaccine Candidate in Chickens |
| title_full_unstemmed | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated <i>Salmonella</i> Enteritidis Vaccine Candidate in Chickens |
| title_short | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated <i>Salmonella</i> Enteritidis Vaccine Candidate in Chickens |
| title_sort | evaluation of immune responses and protective efficacy of a novel live attenuated i salmonella i enteritidis vaccine candidate in chickens |
| topic | <i>Salmonella</i> Enteritidis live attenuated vaccine immune response protective efficacy |
| url | https://www.mdpi.com/2076-393X/10/9/1405 |
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