Analysis of maize profilin-4 isoform as an allergen
Profilin is an actin monomer-binding protein that controls the dynamic turnover of actin filaments and is ubiquitously present in different organisms ranging from prokaryotes to higher eukaryotes. Maize (Zea mays) profilin-4 isoform is a pollen-specific protein. Birch profilin isoform is a known all...
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| Format: | Article |
| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2019-12-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=61795 |
| _version_ | 1818342256367632384 |
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| author | Saruar Alam Md. Kamrul Hasan Md. Faruk Hossain |
| author_facet | Saruar Alam Md. Kamrul Hasan Md. Faruk Hossain |
| author_sort | Saruar Alam |
| collection | DOAJ |
| description | Profilin is an actin monomer-binding protein that controls the dynamic turnover of actin filaments and is ubiquitously present in different organisms ranging from prokaryotes to higher eukaryotes. Maize (Zea mays) profilin-4 isoform is a pollen-specific protein. Birch profilin isoform is a known allergen but maize profilin is yet to be characterized. Due to the high cultivation rate of maize, the analysis of the properties of maize profilin-4 isoform as an allergen is a demand of time for developing an effective immune therapy. Here, we analyzed the allergenic potency of profilin-4 by studying its physicochemical properties, including molecular weight (~14kD) and theoretical pI (4.63). We tested the potential B cell epitope candidates of profilin-4 using different immune-informatics tools housed at IEDB analysis resource. For the B cell epitope prediction, potential antigenic sites on the protein surface were predicted by both propensity scale and machine learning method followed by their mapping of 3D structure prediction. Our findings suggest that profilin-4 is a potential allergen and is able to induce allergic responses. [ J Adv Biotechnol Exp Ther 2019; 2(3.000): 134-139] |
| first_indexed | 2024-12-13T16:11:48Z |
| format | Article |
| id | doaj.art-5d6a1c5c094d47ec9e2aaf0e27b09580 |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-12-13T16:11:48Z |
| publishDate | 2019-12-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-5d6a1c5c094d47ec9e2aaf0e27b095802022-12-21T23:38:55ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602019-12-012313413910.5455/jabet.2019.d3661795Analysis of maize profilin-4 isoform as an allergenSaruar Alam0Md. Kamrul Hasan1Md. Faruk Hossain2University of Dhaka, Dhaka, Bangladesh University of Dhaka, Dhaka, Bangladesh St. John s University, Queens, NY, USAProfilin is an actin monomer-binding protein that controls the dynamic turnover of actin filaments and is ubiquitously present in different organisms ranging from prokaryotes to higher eukaryotes. Maize (Zea mays) profilin-4 isoform is a pollen-specific protein. Birch profilin isoform is a known allergen but maize profilin is yet to be characterized. Due to the high cultivation rate of maize, the analysis of the properties of maize profilin-4 isoform as an allergen is a demand of time for developing an effective immune therapy. Here, we analyzed the allergenic potency of profilin-4 by studying its physicochemical properties, including molecular weight (~14kD) and theoretical pI (4.63). We tested the potential B cell epitope candidates of profilin-4 using different immune-informatics tools housed at IEDB analysis resource. For the B cell epitope prediction, potential antigenic sites on the protein surface were predicted by both propensity scale and machine learning method followed by their mapping of 3D structure prediction. Our findings suggest that profilin-4 is a potential allergen and is able to induce allergic responses. [ J Adv Biotechnol Exp Ther 2019; 2(3.000): 134-139]http://www.ejmanager.com/fulltextpdf.php?mno=61795profilin-4allergenicityzea maysallergenin silicoepitope |
| spellingShingle | Saruar Alam Md. Kamrul Hasan Md. Faruk Hossain Analysis of maize profilin-4 isoform as an allergen Journal of Advanced Biotechnology and Experimental Therapeutics profilin-4 allergenicity zea mays allergen in silico epitope |
| title | Analysis of maize profilin-4 isoform as an allergen |
| title_full | Analysis of maize profilin-4 isoform as an allergen |
| title_fullStr | Analysis of maize profilin-4 isoform as an allergen |
| title_full_unstemmed | Analysis of maize profilin-4 isoform as an allergen |
| title_short | Analysis of maize profilin-4 isoform as an allergen |
| title_sort | analysis of maize profilin 4 isoform as an allergen |
| topic | profilin-4 allergenicity zea mays allergen in silico epitope |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=61795 |
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