Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age
Amino acids, as nutrients, are expected to improve sleep disorders. This study aimed to evaluate the generation- and age-dependent sleep-improving effects of γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) coadministration. The differentially expressed genes and generation-related behavio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/18/9822 |
| _version_ | 1797518888770994176 |
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| author | Yejin Ahn Ki-Bae Hong Suhyeon Kim Hyung Joo Suh Kyungae Jo |
| author_facet | Yejin Ahn Ki-Bae Hong Suhyeon Kim Hyung Joo Suh Kyungae Jo |
| author_sort | Yejin Ahn |
| collection | DOAJ |
| description | Amino acids, as nutrients, are expected to improve sleep disorders. This study aimed to evaluate the generation- and age-dependent sleep-improving effects of γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) coadministration. The differentially expressed genes and generation-related behavior after the administration of a GABA/5-HTP mixture were measured in a <i>Drosophila</i> model, while age-related changes in gene expression and oxidative stress-related parameters were measured in a mouse model. The GABA/5-HTP-treated group showed significant behavioral changes compared to the other groups. Sequencing revealed that the GABA/5-HTP mixture influenced changes in nervous system-related genes, including those involved in the regulation of the expression of behavioral and synaptic genes. Additionally, total sleep time increased with age, and nighttime sleep time in the first- and third-generation flies was significantly different from that of the control groups. The GABA/5-HTP mixture induced significant changes in the expression of sleep-related receptors in both models. Furthermore, the GABA/5-HTP mixture reduced levels of ROS and ROS reaction products in an age-dependent manner. Therefore, the increase in behavioral changes caused by GABA/5-HTP mixture administration was effective in eliminating ROS activity across generations and ages. |
| first_indexed | 2024-03-10T07:35:42Z |
| format | Article |
| id | doaj.art-5d6bf23ccba0485da5e102bb9b18a77b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T07:35:42Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5d6bf23ccba0485da5e102bb9b18a77b2023-11-22T13:28:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-012218982210.3390/ijms22189822Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and AgeYejin Ahn0Ki-Bae Hong1Suhyeon Kim2Hyung Joo Suh3Kyungae Jo4Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, KoreaDepartment of Food Science and Nutrition, Jeju National University, Jeju 63243, KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, KoreaAmino acids, as nutrients, are expected to improve sleep disorders. This study aimed to evaluate the generation- and age-dependent sleep-improving effects of γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) coadministration. The differentially expressed genes and generation-related behavior after the administration of a GABA/5-HTP mixture were measured in a <i>Drosophila</i> model, while age-related changes in gene expression and oxidative stress-related parameters were measured in a mouse model. The GABA/5-HTP-treated group showed significant behavioral changes compared to the other groups. Sequencing revealed that the GABA/5-HTP mixture influenced changes in nervous system-related genes, including those involved in the regulation of the expression of behavioral and synaptic genes. Additionally, total sleep time increased with age, and nighttime sleep time in the first- and third-generation flies was significantly different from that of the control groups. The GABA/5-HTP mixture induced significant changes in the expression of sleep-related receptors in both models. Furthermore, the GABA/5-HTP mixture reduced levels of ROS and ROS reaction products in an age-dependent manner. Therefore, the increase in behavioral changes caused by GABA/5-HTP mixture administration was effective in eliminating ROS activity across generations and ages.https://www.mdpi.com/1422-0067/22/18/9822γ-aminobutyric acid5-hydroxytryptophansleepreactive oxygen speciesgenerationage |
| spellingShingle | Yejin Ahn Ki-Bae Hong Suhyeon Kim Hyung Joo Suh Kyungae Jo Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age International Journal of Molecular Sciences γ-aminobutyric acid 5-hydroxytryptophan sleep reactive oxygen species generation age |
| title | Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age |
| title_full | Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age |
| title_fullStr | Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age |
| title_full_unstemmed | Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age |
| title_short | Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age |
| title_sort | changes in locomotor activity and oxidative stress related factors after the administration of an amino acid mixture by generation and age |
| topic | γ-aminobutyric acid 5-hydroxytryptophan sleep reactive oxygen species generation age |
| url | https://www.mdpi.com/1422-0067/22/18/9822 |
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