Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina Breed
The objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve goat meat evaluation. Insulin like growth factor -1 play an important stimulator role in skeletal growth, cell differentiation and metabolism. Also it has an impor...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2023-09-01
|
| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1018 |
| _version_ | 1797215421389078528 |
|---|---|
| author | Cristina Lazar Rodica Stefania Pelmus Alexandru Mihail Gras Mircea Catalin Rotar Elena Ghiţă |
| author_facet | Cristina Lazar Rodica Stefania Pelmus Alexandru Mihail Gras Mircea Catalin Rotar Elena Ghiţă |
| author_sort | Cristina Lazar |
| collection | DOAJ |
| description | The objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve goat meat evaluation. Insulin like growth factor -1 play an important stimulator role in skeletal growth, cell differentiation and metabolism. Also it has an important role in control of hair cycles and it is involved in development of wool fiber. Therefore, it is considered as an appropriate candidate gene for meat quality evaluation. Blood samples were collected from 12 Carpatina kids and DNA was extracted using, Wizard Genomic DNA Purification Kit. Polymorphism of IGF-1 was determined by PCR amplification followed by RFLP method using restriction enzyme Hae III. In the present study heterozygous individuals were identified with genotype BC (25%) and homozygous individuals for the genotype BB (75%). B allele frequency was 0.87 and for allele C was 0.13. Observed and expected values of IGF-1 genotypes were found in Hardy Weinberg equilibrium, after χ2 test was calculated. Further investigation is necessary to be carried out on a high number of animals in order to discover other mutations correlated with meat quality in Carpatina goat. |
| first_indexed | 2024-04-24T11:29:48Z |
| format | Article |
| id | doaj.art-5d6d90c120ee4d83b428dd64108d2efb |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:29:48Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-5d6d90c120ee4d83b428dd64108d2efb2024-04-10T10:54:04ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-0151197971018Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina BreedCristina Lazar0Rodica Stefania Pelmus1Alexandru Mihail Gras2Mircea Catalin Rotar3Elena Ghiţă4National Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaThe objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve goat meat evaluation. Insulin like growth factor -1 play an important stimulator role in skeletal growth, cell differentiation and metabolism. Also it has an important role in control of hair cycles and it is involved in development of wool fiber. Therefore, it is considered as an appropriate candidate gene for meat quality evaluation. Blood samples were collected from 12 Carpatina kids and DNA was extracted using, Wizard Genomic DNA Purification Kit. Polymorphism of IGF-1 was determined by PCR amplification followed by RFLP method using restriction enzyme Hae III. In the present study heterozygous individuals were identified with genotype BC (25%) and homozygous individuals for the genotype BB (75%). B allele frequency was 0.87 and for allele C was 0.13. Observed and expected values of IGF-1 genotypes were found in Hardy Weinberg equilibrium, after χ2 test was calculated. Further investigation is necessary to be carried out on a high number of animals in order to discover other mutations correlated with meat quality in Carpatina goat.https://spasb.ro/index.php/public_html/article/view/1018insulin growth factorgoatmeatevaluationqualitypolymorphism |
| spellingShingle | Cristina Lazar Rodica Stefania Pelmus Alexandru Mihail Gras Mircea Catalin Rotar Elena Ghiţă Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina Breed Scientific Papers Animal Science and Biotechnologies insulin growth factor goat meat evaluation quality polymorphism |
| title | Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina Breed |
| title_full | Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina Breed |
| title_fullStr | Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina Breed |
| title_full_unstemmed | Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina Breed |
| title_short | Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina Breed |
| title_sort | identification of igf 1 gene polymorphism using pcr rflp for improving goat meat evaluation in carpatina breed |
| topic | insulin growth factor goat meat evaluation quality polymorphism |
| url | https://spasb.ro/index.php/public_html/article/view/1018 |
| work_keys_str_mv | AT cristinalazar identificationofigf1genepolymorphismusingpcrrflpforimprovinggoatmeatevaluationincarpatinabreed AT rodicastefaniapelmus identificationofigf1genepolymorphismusingpcrrflpforimprovinggoatmeatevaluationincarpatinabreed AT alexandrumihailgras identificationofigf1genepolymorphismusingpcrrflpforimprovinggoatmeatevaluationincarpatinabreed AT mirceacatalinrotar identificationofigf1genepolymorphismusingpcrrflpforimprovinggoatmeatevaluationincarpatinabreed AT elenaghita identificationofigf1genepolymorphismusingpcrrflpforimprovinggoatmeatevaluationincarpatinabreed |